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Green fluorescence protein (GFP) is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light when excited by blue light. GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. Other applications of GFP include assessment of protein protein interactions through the yeast two hybrid system and measurement of distance between proteins through fluorescence energy transfer (FRET) protocols.

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• SciCrunch
• 11 years ago - submitted by guifa li

In order to obtain the accurate results that unbiased stereology is known for, it is important to follow certain practices, described in the literature, during tissue preparation. MBF has Ph.D. staff scientists who can give you practical support for all aspects of stereologic experimental design—from the tissue processing stage all the way to interpreting the results. Additionally, MBF provides you with a host of expert stereological support that includes detailed software workflows within Stereo Investigator, extensive on-line documentation, videos and webinars. All of this is available to help guide you through the steps of a stereological study that are briefly outlined below.

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• SciCrunch
• 11 years ago - submitted by Alessandro Bilella

The antibody was purified from antisera by immunoaffinity chromatography using antigenscoupled to agarose beads. Application:1:500 - 1:5000 for immunohisto/cytochemistry 1:5000 - 1:100000 for ELISA and Western blotting with chromogenic substrate.

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• SciCrunch
• 11 years ago - submitted by Zizhi Cui

Secondary Antibody.Used for WB（1:10000 - 1:200000）、ELISA（1:5000 - 1:100000） IHC（1:500 - 1:5000）.

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• SciCrunch
• 11 years ago - submitted by Zizhi Cui

Mouse Monoclonal to beta Actin antibody,used for WB ELISA Dot Blot IHC IF.

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• SciCrunch
• 11 years ago - submitted by Zizhi Cui

This toolkit is built for mapping brain entropy using fMRI. It uses several fuctions from ASLtbx

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• SciCrunch
• 11 years ago - submitted by Kristen Jensen

An automated scanning and imaging software that allows for real-time mosaic imaging. This software allows the user to survey, relocate, print, and save microscope images.

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• RRID-Legacy
• 11 years ago - submitted by Jessica Cabotaje

Rhodamine phalloidin is a high-affinity F-actin probe conjugated to the red-orange fluorescent dye, tetramethylrhodamine (TRITC).

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• RRID-Legacy
• 11 years ago - by Anonymous

TSA® Cyanine 3 (Cy3) detection kit, for amplification of signal in immunohistochemistry (IHC), immunofluorescence (IF) or in situ hybridization protocols. This kit amplifies by depositing extra Cy3 in the localized area of the probe or antibody.

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• RRID-Legacy
• 11 years ago - by Anonymous

Veliparib is a potential anti-cancer drug acting as a PARP inhibitor. It kills cancer cells by blocking a protein called PARP, thereby preventing the repair of DNA or genetic damage in cancer cells and possibly making them more susceptible to anticancer treatments. Veliparib may make whole brain radiation treatment work more effectively against brain metastases from NSCLC. It inhibits both PARP1 and PARP2

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• RRID-Legacy
• 11 years ago - by Anonymous

A software for acquiring, enhancing, and analyzing images with true color imaging and correction.

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• RRID-Legacy
• 11 years ago - submitted by Jessica Cabotaje

Opti-MEM® I Reduced-Serum Medium is an improved Minimal Essential Medium (MEM) that allows for a reduction of Fetal Bovine Serum supplementation by at least 50% with no change in growth rate or morphology. Opti-MEM® I medium is also recommended for use with cationic lipid transfection reagents, such as Lipofectamine™ reagent. Opti-MEM® I medium can be used with a variety of suspension and adherent mammalian cells, including Sp2, AE-1, CHO, BHK-21, HEK, and primary fibroblasts. We offer a variety of Opti-MEM® I modifications for a range of cell culture applications.

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• RRID-Legacy
• 11 years ago - by Anonymous

Mammalian expressed C-terminal FLAG-tagged mouse CTRP3 recombinant proteins

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• RRID-Legacy
• 11 years ago - by Anonymous

xFITOM is a fully customizable program for locating transcription factor-binding sites in genomic sequences

• RRID-Legacy
• 11 years ago - by Anonymous

Antibody: Anti-Dimethyl Histone H3 (Lys4) Antibody (H3K4me2 antibody) Reference: 05-1338 (Milipore) clone CMA303

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• NIF
• 11 years ago - by Anonymous

A detection and analysis program designed to track and aid in the interpretation of spontaneous activities. MiniAnalysis detects various types of peaks, including spontaneous miniature synaptic currents and potentials, action potential spikes, calcium imaging peaks, amperometric peaks, ECG peaks, and the like. The software also performs post detection analysis and provides users with essential plots.

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• SciCrunch
• 11 years ago - submitted by Gabrielle Pine

Affinity purified rabbit polyclonal antibody to mCherry, concentrated to 1mg/mL in PBS, 50% glycerol and 5mM sodium azide.

• SciCrunch
• 11 years ago - submitted by Gerry Shaw

Analyze provides an integrated, comprehensive set of tools for the visualization, segmentation, registration and analysis of multidimensional biomedical imaging data. With Analyze, both anatomic structure and associated function can be studied and fused together for synergistic display and measurement of important structure-to-function relationships.

• RRID-Legacy
• 11 years ago - by Anonymous

Guidance in protection against potential genomic sequence errors in public eutherian genomic sequences.

• RRID-Legacy
• 11 years ago - by Anonymous

A program to improve the measurements of the autoantibodies predictive of type 1 diabetes.

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• SciCrunch
• 11 years ago - submitted by Kristen Jensen