Curator Dashboard

A database which indexes preprints from arXiv q-bio, PeerJ Preprints, Figshare, bioRxiv, F1000Research, and The Winnower.

• Resource
• SciCrunch
• 11 years ago - submitted by Jamie Li

A set of software tools used for morphometric analysis and visualization of the 3D structure of neurons. Py3DN works exclusively with data collected with the commercial system Neurolucida.

• RRID-Legacy
• 11 years ago - submitted by David Kennedy

Clinical evidence has shown that physical exercise during pregnancy may alter brain devel- opment and improve cognitive function of offspring. However, the mechanisms through which maternal exercise might promote such effects are not well understood. The present study examined levels of brain-derived neurotrophic factor (BDNF) and absolute cell num- bers in the hippocampal formation and cerebral cortex of rat pups born from mothers exer- cised during pregnancy. Additionally, we evaluated the cognitive abilities of adult offspring in different behavioral paradigms (exploratory activity and habituation in open field tests, spatial memory in a water maze test, and aversive memory in a step-down inhibitory avoid- ance task). Results showed that maternal exercise during pregnancy increased BDNF lev- els and absolute numbers of neuronal and non-neuronal cells in the hippocampal formation of offspring. No differences in BDNF levels or cell numbers were detected in the cerebral cortex. It was also observed that offspring from exercised mothers exhibited better cognitive performance in nonassociative (habituation) and associative (spatial learning) mnemonic tasks than did offspring from sedentary mothers. Our findings indicate that maternal exer- cise during pregnancy enhances offspring cognitive function (habituation behavior and spa- tial learning) and increases BDNF levels and cell numbers in the hippocampal formation of offspring.

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• RRID-Legacy
• 11 years ago - submitted by James Wilson

Antibody to detect rat Lysozyme-like protein by Western blotting and Immunofluorescence

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• SciCrunch
• 11 years ago - submitted by suresh yenugu

A database which provides results of comprehensive immunogenomic analyses of next generation sequencing data for 19 solid cancers from The Cancer Genome Atlas and other datasources. The database can be queried for the gene expression of specific immune-related gene sets, cellular composition of immune infiltrates (characterized using gene set enrichment analyses and deconvolution), neoantigens and cancer-germline antigens, HLA types, and tumor heterogeneity (estimated from cancer cell fractions). It also provides survival analyses for different types immunological parameters.

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• RRID-Legacy
• 11 years ago - by Anonymous

A commercial biomaterial analysis service dedicated to developing antibody protein sequencing and characterization technologies. The main service it offers is antibody de novo sequencing. Both heavy and light chains are directly sequenced using multiple enzyme digestion and high resolution LC-MS/MS. The sequencing process does not require hybridoma, mRNA, or other sequence information. Rapid Novor also offers to make recombinant versions of antibody proteins sent in for sequencing.

• Organization
• SciCrunch
• 11 years ago - submitted by Mingjie Xie

Creative Diagnostics provides a wide range of enzyme-linked immunosorbent assay (ELISA) kits for the detection of hundreds of different proteins and molecules including cytokines, growth factors, markers for infectious diseases, diabetes and tumor, drugs and small molecules, etc. ELISA kits are a quick, convenient, and accurate research tool for the detection and quantitation of targets of interest in cultures and samples. We offer multiple assay formats including traditional sandwich ELISA kits, competitive ELISA kits, as well as cell-based ELISA Kits for many targets. These ELISA kits include pre-coated plates with capture/detection antibodies, standards, buffers and accessory reagents. Each kit goes through fit-for-purpose validation and stability testing to ensure precision, accuracy, sensitivity, specificity, and reproducibility.

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• SciCrunch
• 11 years ago - submitted by Emily Dean

A persistent unique identifier for samples and specimens collected from the natural environment. IGSNs are created by the user and are case sensitive. They should be concise, machine readable, and non-derivable to ensure originality. IGSNs were created and are organized by the IGSN e.V., whose goal is to implement and promote standard methods for locating, identifying, and citing physical samples with confidence by operating an international IGSN registration service with a distributed infrastructure for use by and benefit to its members.

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• SciCrunch
• 11 years ago - submitted by Kristen Jensen

A material analysis service of the commercial organization Genetica DNA Laboratories which provides authentication of human cancer cell lines, stem cell lines and xenografts utilizing STR DNA technology.

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• RRID-Legacy
• 11 years ago - submitted by Erin Hall

The ubiquitous genus Alternaria contains many species that are able to invade cereals, oleaginous plants and other crops. Alternaria alternata is considered one of the most important species and can produce several mycotoxins under favourable conditions of temperature and humidity, including the economically important toxins: alternariol (AOH) and alternariol monomethyl ether (AME). The aim of this study was to evaluate the radio-sensitivity of Alternaria alternata spores through different gamma radiation doses. A. alternata growth and the production of AOH and AME were then analyzed. After fungal irradiation with 2 kGy, 5 kGy and 7 kGy, the spores were suspended with sterile distilled water followed by inoculation on wheat grains. The count of the colony-forming units per gram (CFU/g) was performed using Dichloran Rose Bengal Chloramphenicol (DRBC) and Dichloran Chloramphenicol Agar Malt Extract (DCMA); AOH and AME were analyzed using Liquid Chromatography coupled with Mass Spectrometer (LC-MS). Results showed that fungal growth and toxin production increased with the increase of radiation dosage. The implications of these findings in relation to the resistance of A. alternata spores to gamma irradiation are discussed.

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• SciCrunch
• 11 years ago - submitted by karlet danny

The ubiquitous genus Alternaria contains many species that are able to invade cereals, oleaginous plants and other crops. Alternaria alternata is considered one of the most important species and can produce several mycotoxins under favourable conditions of temperature and humidity, including the economically important toxins: alternariol (AOH) and alternariol monomethyl ether (AME). The aim of this study was to evaluate the radio-sensitivity of Alternaria alternata spores through different gamma radiation doses. A. alternata growth and the production of AOH and AME were then analyzed. After fungal irradiation with 2 kGy, 5 kGy and 7 kGy, the spores were suspended with sterile distilled water followed by inoculation on wheat grains. The count of the colony-forming units per gram (CFU/g) was performed using Dichloran Rose Bengal Chloramphenicol (DRBC) and Dichloran Chloramphenicol Agar Malt Extract (DCMA); AOH and AME were analyzed using Liquid Chromatography coupled with Mass Spectrometer (LC-MS). Results showed that fungal growth and toxin production increased with the increase of radiation dosage. The implications of these findings in relation to the resistance of A. alternata spores to gamma irradiation are discussed.

• Resource
• SciCrunch
• 11 years ago - submitted by karlet danny

A toolkit for EEG-fMRI multimodal fusion and fMRI data preprocessing and analysis. NIT allows users to perform batch processing of fMRI data analysis and data preprocessing based on SPM8, as well as parallel computing for data preprocessing, nuisance signals removal, and FCD and FOCA calculating. Users can also use NIT to calculate functional connectivity density and four dimensional (spatio-temporal) consistency of local neural activities.

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• NIF
• 11 years ago - submitted by Maryann Martone

Previous studies have shown that zinc deficiency leads to apoptosis of neuronal precursor cells in vivo and in vitro. In addition to the role of p53 as a nuclear transcription factor in zinc deficient cultured human neuronal precursors (NT-2), we have now identified the translocation of phosphorylated p53 to the mitochondria and p53-dependent increases in the pro-apoptotic mitochondrial protein BAX leading to a loss of mitochondrial membrane potential as demonstrated by a 25% decrease in JC-1 red:green fluorescence ratio. Disruption of mitochondrial membrane integrity was accompanied by efflux of the apoptosis inducing factor (AIF) from the mitochondria and translocation to the nucleus with a significant increase in reactive oxygen species (ROS) after 24 h of zinc deficiency. Measurement of caspase cleavage, mRNA, and treatment with caspase inhibitors revealed the involvement of caspases 2, 3, 6, and 7 in zinc deficiency-mediated apoptosis. Down-stream targets of caspase activation, including the nuclear structure protein lamin and polyADP ribose polymerase (PARP), which participates in DNA repair, were also cleaved. Transfection with a dominant-negative p53 construct and use of the p53 inhibitor, pifithrin- ␮, established that these alterations were largely dependent on p53. Together these data identify a cascade of events involving mitochondrial p53 as well as p53-dependent caspase-mediated mechanisms leading to apoptosis during zinc deficiency.

• NIF
• 11 years ago - submitted by Edwin Levinson

Green fluorescence protein (GFP) is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light when excited by blue light. GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. Other applications of GFP include assessment of protein protein interactions through the yeast two hybrid system and measurement of distance between proteins through fluorescence energy transfer (FRET) protocols.

• Resource
• SciCrunch
• 11 years ago - submitted by guifa li

A research project investigating the difficulties of recovering language after stroke (aphasia). The overall aim of the study is to give future stroke survivors accurate predictions of their aphasia recovery by creating clinical tools and discerning why some patients recover from aphasia better than others.

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• SciCrunch
• 11 years ago - submitted by Kristen Jensen

A data acquisition and analysis software program. The program collects data from sensors and receivers and displays graphical data analysis as well as descriptive statistics.

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• RRID-Legacy
• 11 years ago - submitted by Jessica Cabotaje

A statistical process control (SPC) add-in for Microsoft Excel. The user can select their data and the program will draw the desired chart or run the desired statistical test.

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• RRID-Legacy
• 11 years ago - submitted by Jessica Cabotaje

Software for detecting the onset and completion of freezing behavior of a rodent. It outputs the freezing behaviors as a sequential list, allowing for further statistical analysis. Freezescan detects freezing behaviors, random intervals, interval freezing, and activity levels. It also detects animal activity during the experiment and can output in quantitative and graphical form.

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• RRID-Legacy
• 11 years ago - submitted by Jessica Cabotaje

A single point video tracking system. It uses the center of the mass of the animal and tracks it over the course of the experiment to determine location, distance traveled, velocity etc. Examples of applications include: Open Field, Novel Object Recognition, Maze Analysis, Morris Water Maze, Barnes Maze, Place Preference, etc.

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• RRID-Legacy
• 11 years ago - submitted by Jessica Cabotaje

A software package for quantification of histological sections. This software performs functions including: management and analysis of regions of interest, annotations, statistical analysis, and 3D visualization. These results are edited in an Excel-compatible spreadsheet.

• Resource
• RRID-Legacy
• 11 years ago - submitted by Jessica Cabotaje