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Previous studies have shown that zinc deficiency leads to apoptosis of neuronal precursor cells in vivo and in vitro. In addition to the role of p53 as a nuclear transcription factor in zinc deficient cultured human neuronal precursors (NT-2), we have now identified the translocation of phosphorylated p53 to the mitochondria and p53-dependent increases in the pro-apoptotic mitochondrial protein BAX leading to a loss of mitochondrial membrane potential as demonstrated by a 25% decrease in JC-1 red:green fluorescence ratio. Disruption of mitochondrial membrane integrity was accompanied by efflux of the apoptosis inducing factor (AIF) from the mitochondria and translocation to the nucleus with a significant increase in reactive oxygen species (ROS) after 24 h of zinc deficiency. Measurement of caspase cleavage, mRNA, and treatment with caspase inhibitors revealed the involvement of caspases 2, 3, 6, and 7 in zinc deficiency-mediated apoptosis. Down-stream targets of caspase activation, including the nuclear structure protein lamin and polyADP ribose polymerase (PARP), which participates in DNA repair, were also cleaved. Transfection with a dominant-negative p53 construct and use of the p53 inhibitor, pifithrin- ␮, established that these alterations were largely dependent on p53. Together these data identify a cascade of events involving mitochondrial p53 as well as p53-dependent caspase-mediated mechanisms leading to apoptosis during zinc deficiency.

• NIF
• 11 years ago - submitted by Edwin Levinson

Green fluorescence protein (GFP) is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light when excited by blue light. GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. Other applications of GFP include assessment of protein protein interactions through the yeast two hybrid system and measurement of distance between proteins through fluorescence energy transfer (FRET) protocols.

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• SciCrunch
• 11 years ago - submitted by guifa li

A research project investigating the difficulties of recovering language after stroke (aphasia). The overall aim of the study is to give future stroke survivors accurate predictions of their aphasia recovery by creating clinical tools and discerning why some patients recover from aphasia better than others.

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• SciCrunch
• 11 years ago - submitted by Kristen Jensen

A data acquisition and analysis software program. The program collects data from sensors and receivers and displays graphical data analysis as well as descriptive statistics.

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• RRID-Legacy
• 11 years ago - submitted by Jessica Cabotaje

A statistical process control (SPC) add-in for Microsoft Excel. The user can select their data and the program will draw the desired chart or run the desired statistical test.

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• RRID-Legacy
• 11 years ago - submitted by Jessica Cabotaje

Software for detecting the onset and completion of freezing behavior of a rodent. It outputs the freezing behaviors as a sequential list, allowing for further statistical analysis. Freezescan detects freezing behaviors, random intervals, interval freezing, and activity levels. It also detects animal activity during the experiment and can output in quantitative and graphical form.

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• RRID-Legacy
• 11 years ago - submitted by Jessica Cabotaje

A single point video tracking system. It uses the center of the mass of the animal and tracks it over the course of the experiment to determine location, distance traveled, velocity etc. Examples of applications include: Open Field, Novel Object Recognition, Maze Analysis, Morris Water Maze, Barnes Maze, Place Preference, etc.

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• RRID-Legacy
• 11 years ago - submitted by Jessica Cabotaje

A software package for quantification of histological sections. This software performs functions including: management and analysis of regions of interest, annotations, statistical analysis, and 3D visualization. These results are edited in an Excel-compatible spreadsheet.

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• RRID-Legacy
• 11 years ago - submitted by Jessica Cabotaje

A software for (scanning) transmission electron microscope experimental control and analysis.

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• RRID-Legacy
• 11 years ago - submitted by Jessica Cabotaje

Software suite for creating, editing, composing, and converting bitmap images. It can read and write images in over 200 formats including PNG, JPEG, JPEG-2000, GIF, TIFF, DPX, EXR, WebP, Postscript, PDF, and SVG. The user can use this software to resize, flip, mirror, rotate, distort, shear and transform images, adjust image colors, apply various special effects, or draw text, lines, polygons, ellipses and Bézier curves.

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• RRID-Legacy
• 11 years ago - submitted by Jessica Cabotaje

A software for primer design. The user can choose the target sequence or gene and design primers for the real-time PCR. After, the user can examine the results and choose from the recommended probes., THIS RESOURCE IS NO LONGER IN SERVICE. Documented on September 16,2025.

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• RRID-Legacy
• 11 years ago - submitted by Jessica Cabotaje

A software for acquiring and analyzing flow cytometry data. The user can create plots with elements like regions, gates, statistics, markers, and annotated text. The software can also be used for batch analysis or quality control of analysis. Results can be exported and saved., THIS RESOURCE IS NO LONGER IN SERVICE. Documented on September 16,2025.

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• RRID-Legacy
• 11 years ago - submitted by Jessica Cabotaje

A bioinformatics software for editing, annotating, and analyzing DNA, RNA, and amino acid sequences. It includes analytical tools and can be expanded with homology search, multiple alignment and base calling, and sequence assembly (Phred/Phrap) modules.

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• RRID-Legacy
• 11 years ago - submitted by Jessica Cabotaje

A computer package for performing analyses of trait evolution among groups of species for which a phylogeny or sample of phylogenies is available. It incorporates earlier programs including Multistate, Discrete and Continuous. BayesTraits can be applied to the analysis of traits that adopt a discrete number of states, or to the analysis of continuously varying traits. Hypotheses can be tested about models of evolution, ancestral states and correlations among pairs of traits., THIS RESOURCE IS NO LONGER IN SERVICE. Documented on September 16,2025.

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• RRID-Legacy
• 11 years ago - submitted by Jessica Cabotaje

A software for analyzing real-time PCR data and quantifying DNA targets. It can perform copy number analysis, report confidence in copy number, display data in table and graphic format, change analysis parameters, and view data analysis information for multiple experiments at once.

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• RRID-Legacy
• 11 years ago - submitted by Jessica Cabotaje

A quantitative proteomics software package for analyzing large-scale mass-spectrometric data sets. It is a set of algorithms that include peak detection and scoring of peptides, mass calibration, database searches for protein identification, protein quantification, and provides summary statistics.

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• RRID-Legacy
• 11 years ago - submitted by Jessica Cabotaje

An application for extracting numerical data from pictures. It can be used to view the original data values (x,y) from printed graphs. The user can perform basic operations like cut, copy, paste as well as select axes colors, zoom in or out, remove noise, re-calibrate the y-axis, and insert, edit or move points. It offers support for GIF, JPEG, and PNG files.

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• RRID-Legacy
• 11 years ago - submitted by Jessica Cabotaje

A statistical software package that allows the user to import data, create graphs, and perform statistical analysis. It can perform basic statistical tests like descriptive statistics, hypothesis tests, confidence intervals, and normality tests as well as regression and ANOVA and quality assessment.

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• RRID-Legacy
• 11 years ago - submitted by Jessica Cabotaje

A software for analyzing animal movement on videos and images. The user can define the animal's joints so the software can analyze using a tracking algorithm.

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• RRID-Legacy
• 11 years ago - submitted by Jessica Cabotaje

A software tool for automated analysis and quantification of fluorescent microscopy images of nerve cells in vivo and in vitro. The tool also can be used for analysis of cell morphology, neurite length analysis for sections with no cell body, and ganglion explant analysis.

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• RRID-Legacy
• 11 years ago - submitted by Jessica Cabotaje