Curator Dashboard

Core facility that provides general synthetic and medicinal chemistry resources and expertise to the biomedical research community. Services include chemistry consulting for projects, grant applications, synthetic chemistry, medicinal chemistry, and analytical chemistry, as well as access to equipment for synthesis, purification, and analysis.

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• SciCrunch
• 10 years ago - submitted by Kristen Jensen

Core facility that provides diverse screening instrumentation, compound libraries and expertise on high throughput screens and automation in support of collaborative, and user- or facility-driven screening projects. The facility collaborates with a number of partners to develop molecular therapies. Services available include consultation for high throughput screening approaches, conversion of assays to automation compatible formats and data analysis.

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• SciCrunch
• 10 years ago - submitted by Kristen Jensen

Core facility that aims to develop cell based disease models for screening and testing small molecules and drugs, and specializes in culture, scaling, and directed differentiation of human embryonic and induced pluripotent stem cells. It provides resources and expertise to researchers at the Institute and to their external partners to facilitate development of stem cell based assays and patient-specific disease models in screenable formats.

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• SciCrunch
• 10 years ago - submitted by Kristen Jensen

Core facility that provides access to the HTS plate and liquid handling infrastructure of the screening center, as well as the screening center�s cell culture facility. Other services include assay development, screening, and data analysis/mining expertise and services for high content screens. Consultation from the team is available for high content image-based screens including sample preparation, image acquisition, image analysis, image data management, and algorithm development.

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• SciCrunch
• 10 years ago - submitted by Kristen Jensen

THIS RESOURCE IS NO LONGER IN SERVICE. Documented on 11132025. Facility that provides database development and management and bioinformatic network building services by utilizing on-site hardware and software. Three members of the facility are available to assist researchers with advanced bioinformatics and biostatistics analysis to help put data into biological context across various disease areas to create testable hypotheses and understand biology of the process. The bulk of support includes connecting functional genomic data with pathways and networks, connecting gene/protein expression and disease state and consultations on statistical aspects of the research with the team statistician.

• Resource
• SciCrunch
• 10 years ago - submitted by Kristen Jensen

Core facility that performs design, development, validation and pilot screening of biological high-throughput assays. It provides services such as designing and developing de-novo assays, converting low-throughput lab protocols (e.g. cuvette- or gel-based) into plate-based HTS assays, and access to major robotic equipment and resources. The facility also provides direct assistance with preparing screening-related grants, guidance through assay development stages, help in designing testing funnels, and generation of preliminary data.

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• SciCrunch
• 10 years ago - submitted by Kristen Jensen

Core facility that provides sequencing services, access to equipment and consultation on experimental design and data analysis. Available instruments include the Life Technologies Ion Torrent PGM and Ion Proton sequencers. Core also provides amplification-free analysis of RNA expression using the NanoString nCounter, and additionally provides sequencing and analysis services to investigators outside of SBP. Included in the cost of sequencing is basic bioinformatic analysis (SNP/InDel calling, transcript abundance). Lastly, the core also provides advice on experimental design, and guidance on the capabilities of next-generation sequencing.

• Resource
• SciCrunch
• 10 years ago - submitted by Kristen Jensen

Facility that provides mass spectrometry-based proteomics services with methods tailored to the needs of scientific investigators for routine mass spectrometric analysis of peptides and proteins, identification of novel proteins, characterization of post-translational modifications, and quantitative proteomics analysis of simple to complex biological samples. The facility identifies, validates and develops new methods designed for the characterization and identification of biologically important proteins. It also supports users with software applications and development, data analysis and experimental design of proteomics studies.

• Resource
• SciCrunch
• 10 years ago - submitted by Kristen Jensen

Core provides viral vector based gene/shRNA delivery technology. Its portfolio ranges from lentivirus, retrovirus, adenovirus, adeno-associated virus, zika virus, coronavirus, sindbis virus and vesicular stomatitis virus products as well as customized "The Works" viral vector construction and swapping service packages. There are over 50 ready-to-transduce reporter viruses available for live cell imaging and 2D/3D cell-based assays. For custom virus production, we can achieve high-titer, top quality viral particles suitable for in vitro and in vivo studies. The automated mini-scale preparations of vector plasmids and viruses in 96-well format can immediately allow downstream viral array screening. The core also extends technical expertise in non-viral nanoparticles which includes large extracellular vesicles and exosome purification, analysis and engineering.

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• SciCrunch
• 10 years ago - submitted by Kristen Jensen

Facility that provides full-service microarray-based global analysis of gene expression in RNA samples from humans or mice using an Illumina platform. Services include RNA sample quality and quantity analysis, microarray analysis, and Q-PCR service and analysis, as well as special services such as RNA purification and Copy Number Variation analysis.

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• SciCrunch
• 10 years ago - submitted by Kristen Jensen

Facility that provides a variety of analytical services focused on biophysical characterization of structural and functional properties of proteins in solution, under native, non-denaturing conditions. Examples of services include quality control of protein samples (folding, stability, aggregation); measuring molecular weight of proteins, protein complexes, oligomers and assemblies; charcaterizing protein conformation and shape in solution; determining oligomeric state of protein (including stoichiometry and Kd for self-association) and measuring protein binding to proteins, peptides, small molecules, compounds, metals, nucleotides and other ligands (including determination of equilibrium (Kd) and kinetic rate (kon, koff) constants, stoichiometry, binding enthalpy and entropy).

• Resource
• SciCrunch
• 10 years ago - submitted by Kristen Jensen

Facility that acts as a centralized shared resource for NMR studies on proteins, peptides, small molecules, and carbohydrates in solution or in solid state. It provides instrumentation and expertise for NMR data collection. It also provides consultation with investigators on the feasibility of NMR for structural studies of protein candidates, as well as the optimal method to obtain solution structures and binding information by multi-dimensional NMR techniques. It can also train users in basic spectrometer operations, trouble-shoot for instrumental and operational problems, and set up NMR experiments for users as requested.

• Resource
• SciCrunch
• 10 years ago - submitted by Kristen Jensen

Facility equipped with X-ray crystallography equipment, such as the Rigaku FR-E SuperBright Ultra high-intensity rotating anode generator. The instrument is inteded for self-usage by trained users. It utilizes both ports with two independent diffraction stations that can run simultaneously. Each station is equipped with an X-stream cryogenic system for standard low temperature (~100 K) data collection. The system is used to collect high resolution diffraction data from native and inhibitor-bound crystals or to test and optimize diffraction quality (cryo-protectant conditions, etc.) and screen for further data collection at synchrotron sources.

• Resource
• SciCrunch
• 10 years ago - submitted by Kristen Jensen

Core provides the infrastructure for cell-based gain-of-function (ORF, CRISPRa and CRISPRon) and loss-of-functioning (siRN, miRNA, shRNA, CRSIPRko, CRISPRi and CRISPRoff), libraries screening services, starting with assay development and carrying all the way through verification of identified targets. The large scale screens is accomplished via ready-to-use genome-wide or pathway-specific library platforms with choice of arrayed or pooled libraries. The FG core facility is equipped to perform custom CRISPR gene activation, knock-down, knock-out and editing services. We also adopt CRISPR nucleotide base editing technology and offer custom built CRISPR domain libraries. This approach can reveal the specific domain of a target protein interacting with another molecule, such as protein, IncRNA or drug in a native cell cellular environment.

• Resource
• SciCrunch
• 10 years ago - submitted by Kristen Jensen

Facility that provides analysis of animal models of human cancer and other diseases. It provides investigators access to a wide variety of human cancer cell lines for xenograft studies, some primary human xenograft models and additional transgenic mouse solid tumors and leukemia models. The core also offers serial passaging of tumors and derivation of 2D and 3D cultures from xenograft tumors, including patient-derived xenograft (PDX) models. These short-term cell cultures established from PDXs enable in vitro analysis including high throughput screening with compounds or RNAi for functional characterization.

• Resource
• SciCrunch
• 10 years ago - submitted by Kristen Jensen

Facility that provides standard and customized research specific histology services including tissue processing, fixed and frozen sectioning. It also offers routine and special histological stains as well as immunohistochemistry and immunofluorescence. Other services include training for several pieces of common use equipment, such as, paraffin microtome, cryostat, and a cytospin. Training is also provided for basic histological techniques such as perfusion and tissue collection. The tissue acquisition service assists investigators with regulatory/legal compliance, as well as obtains and stores human tissue samples and associated clinical data.

• Resource
• SciCrunch
• 10 years ago - submitted by Kristen Jensen

THIS RESOURCE IS NO LONGER IN SERVICE, documented August 23, 2016. The former functions of this facility are split into two separate operations. The first is the generation and characterization of induced Pluripotent Stem Cells (iPSCs) is now being performed on a collaborative basis for both internal and external investigators with the Snyder lab. The second is a shared laboratory dedicated to the culture and analysis of stem cells that is available to SBP investigators.

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• SciCrunch
• 10 years ago - submitted by Kristen Jensen

Medical research facility that supports research programs by providing access to sophisticated microscopes for digital imaging, as well as training, assistance and guidance. The core facility offers expertise, training and assistance in advanced biological microscopic imaging techniques and use of complex image processing software, use of well-maintained, aligned, and calibrated microscopic equipment, and troubleshooting of equipment and experimental problems.

• Resource
• SciCrunch
• 10 years ago - submitted by Kristen Jensen

Facility that provides access to high-speed cell sorting, analytical flow cytometry, imaging flow cytometry, and validated immune profiling spectral antibody panels. Core staff provide technical expertise in experiment design, data analysis, hardware and software training, operate the facility cell sorters, and are available to assist with analysis experiments for those who prefer to have their samples run by an expert cytometrist.

• Resource
• SciCrunch
• 10 years ago - submitted by Kristen Jensen

Facility that provides Drosophila melanogaster and Caenorhabditis elegans as model systems. It also provides training in how to manipulate the organisms and use equipment and provides technician assistance.

• Resource
• SciCrunch
• 10 years ago - submitted by Kristen Jensen