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Facility equipped with X-ray crystallography equipment, such as the Rigaku FR-E SuperBright Ultra high-intensity rotating anode generator. The instrument is inteded for self-usage by trained users. It utilizes both ports with two independent diffraction stations that can run simultaneously. Each station is equipped with an X-stream cryogenic system for standard low temperature (~100 K) data collection. The system is used to collect high resolution diffraction data from native and inhibitor-bound crystals or to test and optimize diffraction quality (cryo-protectant conditions, etc.) and screen for further data collection at synchrotron sources.

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• SciCrunch
• 10 years ago - submitted by Kristen Jensen

Facility that provides infrastructure for RNAi-based Target Discovery services, from initial feasibility assessment to verification of identified targets. It also serves as a repository for reagents and assays, expertise and other technical information, and works towards technical improvements when necessary. Other available services include consultation, libraries for cloning and screening, and access to equipment such as High-Throughput Microscopes and Plate Readers.

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• SciCrunch
• 10 years ago - submitted by Kristen Jensen

Facility that provides analysis of animal models of human cancer and other diseases. It provides investigators access to a wide variety of human cancer cell lines for xenograft studies, some primary human xenograft models and additional transgenic mouse solid tumors and leukemia models. The core also offers serial passaging of tumors and derivation of 2D and 3D cultures from xenograft tumors, including patient-derived xenograft (PDX) models. These short-term cell cultures established from PDXs enable in vitro analysis including high throughput screening with compounds or RNAi for functional characterization.

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• SciCrunch
• 10 years ago - submitted by Kristen Jensen

Facility that provides standard and customized research specific histology services including tissue processing, fixed and frozen sectioning. It also offers routine and special histological stains as well as immunohistochemistry and immunofluorescence. Other services include training for several pieces of common use equipment, such as, paraffin microtome, cryostat, and a cytospin. Training is also provided for basic histological techniques such as perfusion and tissue collection. The tissue acquisition service assists investigators with regulatory/legal compliance, as well as obtains and stores human tissue samples and associated clinical data.

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• SciCrunch
• 10 years ago - submitted by Kristen Jensen

THIS RESOURCE IS NO LONGER IN SERVICE, documented August 23, 2016. The former functions of this facility are split into two separate operations. The first is the generation and characterization of induced Pluripotent Stem Cells (iPSCs) is now being performed on a collaborative basis for both internal and external investigators with the Snyder lab. The second is a shared laboratory dedicated to the culture and analysis of stem cells that is available to SBP investigators.

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• SciCrunch
• 10 years ago - submitted by Kristen Jensen

Medical research facility that supports research programs by providing access to sophisticated microscopes for digital imaging, as well as training, assistance and guidance. The core facility offers expertise, training and assistance in advanced biological microscopic imaging techniques and use of complex image processing software, use of well-maintained, aligned, and calibrated microscopic equipment, and troubleshooting of equipment and experimental problems.

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• SciCrunch
• 10 years ago - submitted by Kristen Jensen

Facility that provides access to high-speed cell sorting, analytical flow cytometry, imaging flow cytometry, and validated immune profiling spectral antibody panels. Core staff provide technical expertise in experiment design, data analysis, hardware and software training, operate the facility cell sorters, and are available to assist with analysis experiments for those who prefer to have their samples run by an expert cytometrist.

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• SciCrunch
• 10 years ago - submitted by Kristen Jensen

Facility that provides Drosophila melanogaster and Caenorhabditis elegans as model systems. It also provides training in how to manipulate the organisms and use equipment and provides technician assistance.

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• SciCrunch
• 10 years ago - submitted by Kristen Jensen

The central leadership office of the Salk Institute's scientific core facilities. This office provides general management and oversight for administrative, scientific, budgetary and personnel matters for all the scientific core research facilities at the Salk Institute.

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• SciCrunch
• 10 years ago - submitted by Kristen Jensen

Animal imaging and analysis lab that provides imaging and analytical services for SBP investigators. The facility can perform in vivo non-invasive luminescence and fluorescence imaging for xenograft tumor growth and metastasis studies. Analytical equipment supports complete blood cell counts (CBC) and analysis of serum components revealing metabolic or organ stress from small samples of mouse blood.

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• SciCrunch
• 10 years ago - submitted by Kristen Jensen

Core facility that provides services to support stem cell research for Salk Institute researchers and their direct collaborators. The STEM Core provides well-characterized human embryonic stem (hES) cell lines and human induced pluripotent stem (iPS) cell lines, space and equipment for research, as well as training and consultation.

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• SciCrunch
• 10 years ago - submitted by Kristen Jensen

Animal facility that provides housing for specific pathogen free rodents, frogs, and zebrafish. The facility also has trained animal care technicians provide expertise in animal husbandry, transgenic and knockout mouse breeding colony maintenance and assistance with routine technical procedures.

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• SciCrunch
• 10 years ago - submitted by Kristen Jensen

Core facility that provides services such as peptide synthesis, incorporation of non-conventional and/or modified amino acids, HPCL characterization and purification, and Mass spec analysis.

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• SciCrunch
• 10 years ago - submitted by Kristen Jensen

Core facility that provides consultation on the use of viral vector technologies as well as custom design and production services for multiple vector types. The GT3 facilitates the use of these research tools by Salk researchers and others across diverse fields of study such as systems neuroscience, stem cell biology, metabolism, ageing, cancer biology and gene therapy. The GT3 core is a designated Cancer Center Council (C3) core facility. Cancer Center members from participating C3 institutes have preferential rates.

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• SciCrunch
• 10 years ago - submitted by Kristen Jensen

Core houses state-of-the-art equipment in sequencing and genomics, including high-throughput sequencing systems such as Illumina NovaSeq 6000, NextSeq 2000, MiniSeq, and PacBio Sequel IIe system (long-read sequencing), as well as 10x Genomics Chromium Controller single-cell solution, NanoString nCounter MAX Analyzer, and GeoMx Digital Spatial Profiler instrument, along with QuantStudio Real-Time PCR instruments.

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• SciCrunch
• 10 years ago - submitted by Kristen Jensen

Institute dedicated to research in immunology by utilizing the academic and research communities in the La Jolla area as well as technological advances in biomedical research, imaging and analysis. Specific topics include autoimmune disease, cancer, allergies, infectious diseases and inflammation.

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• SciCrunch
• 10 years ago - submitted by Kristen Jensen

Core facility that provides media, plates, and commonly used buffers for molecular biology applications and culturing mammalian cells, C. elegans, yeast, and bacteria. The Media Preparation Core Facility is equipped with 3 automatic plate pourers supported by 2 programmable media kettles capable of producing up to 9 liters of agar or nutrient media per run under gentle and reproducible conditions. Plates are available in 6 cm or 10 cm formats.

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• SciCrunch
• 10 years ago - submitted by Kristen Jensen

Core facilty that provides access and services for analyzing targeted and complex mixtures of protein and small molecules, like metabolites. By utilizing these tools, research on disease states can be augmented by proteomic and metabolomic study.

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• SciCrunch
• 10 years ago - submitted by Kristen Jensen

Core facility established to assist the Salk community with integrating genomics data into their research. The primary focus of the core is to provide analysis support for next-generation sequencing applications.

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• SciCrunch
• 10 years ago - submitted by Kristen Jensen

THIS RESOURCE IS NO LONGER IN SERVICE. Documented on July 5,2024.Core facility that provides Salk researchers access to gene expression analysis services such as whole-genome expression and genotyping using Affymetrix GeneChip technology, high-throughput qPCR and SNP services using Fluidigm Biomark System and Applied Biosystems 7900HT System, and DNA miniprep extraction using Qiagen BioRobot.

• Resource
• SciCrunch
• 10 years ago - submitted by Kristen Jensen