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Recombinant human Osteolectin protein purified from supernatant of 293 cells overexpressing 3'Flag-tagged human Osteolectin (PMID: 27976999)

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• SciCrunch
• 8 years ago - submitted by Bo Shen

Ay and other mutations at the a locus conferring a completely yellow coat color are dominant to all a alleles that produce a darker coat. Hair pigment of Ay heterozygotes is yellow, but eyes are black. Heterozygotes usually become obese and infertile within a few months after birth. Increased adipose tissue mass is due to fat cell hypertrophy, and it has been hypothesized that the obesity results from the observed reduction in hypothalamic norepinephrine and dopamine. Heterozygotes are more susceptible to several kinds of tumors than are normal mice, possibly due, at least in part, to a general increase in cell proliferation that also manifests as a slight increase in lean body mass and skeletal length. Further spleen cells from heterozygotes cause a significantly lower graft vs. host reaction. Mice homozygous for the yellow spontaneous mutation (Ay) die before implantation, or shortly thereafter. The time of death and type of abnormality is, in part, determined by the genetic background on which the mutation is placed. Mice of the KK strain develop diabetes of polygenic origin, and mice of other strains heterozygous for Ay become obese and mildly diabetic. KK.Cg-Ay/J heterozygotes develop hyperglycemia, hyperinsulinemia, glucose intolerance and obesity by eight weeks of age. Studies using isolated adipocytes indicate that tissue responsiveness to insulin is decreased. Histo- and immunocytochemical studies show that the pancreatic islets are hypertrophied and the β-cells are degranulated. These findings suggest that the principal cause of diabetes in these mice is insulin resistance. Body composition analysis shows that both the fat and lean tissue mass are increased compared to non-obese mice, with fat accounting for 30-35% of total body weight. The pleiotropic mutant phenotype of Ay/a mice is attributed to ectopic expression of the agouti protein, while the early embryonic lethality of the Ay mutation in the homozygous state is assumed to result from lack of expression of the Raly gene product (Bultman et al. 1992, Miller et al. 1993, Duhl et al. 1994, Michaud et al. 1993, 1994). That ectopic expression of the agouti protein is probably responsible for the non-lethal aspects of this mutation has been demonstrated by transgenic expression of the protein from a ubiquitous promoter (Klebig et al. 1995, Perry et al. 1995).

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• RRID-Legacy
• 8 years ago - submitted by Thai Tran

The GeneChip Scanner 3000 7G is an extension of the GeneChip Scanner 3000 (GCS 3000) series that allows you to scan next-generation higher-density arrays, including SNP arrays with up to 900,000 SNPs, tiling arrays for transcription and all-exon arrays for whole-genome analysis. The GCS 3000 7G combines space-saving and other design improvements with speed, superior performance and lower scanner-to-scanner variation to dramatically improve throughput and efficiency in your genetic analysis. The GCS 3000 7G also provides enhanced auto-focus and more consistent scanner-to-scanner biological performance to improve data integrity and data sharing between researchers. In addition the GCS 3000 7G has been designed to accommodate future advancements in GeneChip probe array technology. Combined with the GeneChip AutoLoader, the GCS 3000 7G provides sample tracking, temperature control and walk-away freedom from scanning. This scanner is sold with a powerful computer workstation with quad-core Xeon processor loaded with Affymetrix GeneChip Command Console Software (AGCC).

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• RRID-Legacy
• 8 years ago - submitted by Thai Tran

GeneChip™ WT Sense Target Labeling and Control Reagents (900652) and GeneChip WT cDNA Synthesis and Amplification Kits (900672 and 900673) are being discontinued in June 2010. The Whole Transcript (WT) Sense Target Labeling Assay has an updated protocol. The GeneChip WT (Whole Transcript) Terminal Labeling Kit is optimized to be used for the fragmentation and labeling steps of the GeneChip WT Sense Target (ST) Labeling Assay. The WT Terminal Labeling Kit employs a strategy for reproducible DNA fragmentation with a combination of uracil DNA glycosylase (UDG) and apurinic/apyrimidinic endonuclease 1 (APE 1). The typical input material is random-primed, single-stranded DNA Following fragmentation, the resulting fragmented DNA can then be labeled using terminal deoxynucleotidyl transferase (TdT) in the presence of a proprietary biotinylated compound, GeneChip DNA Labeling Reagent. Both components are also provided in the WT Terminal Labeling Kit. The final labeled DNA target is ready to be added to the cocktail for hybridizing to the arrays such as the GeneChip Human Exon 1.0 ST Array.

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• RRID-Legacy
• 8 years ago - submitted by Thai Tran

The RNeasy Mini Kit provides fast purification of high-quality RNA from cells, tissues, and yeast using silica-membrane RNeasy spin columns with a binding capacity of 100 μg RNA. Tissue samples can be conveniently stabilized using RNAlater RNA Stabilization Reagent or Allprotect Tissue Reagent, and efficiently disrupted using a TissueRuptor or TissueLyser system. RNA purification using the RNeasy Mini Kit can be fully automated on the QIAcube. For smaller and larger samples, the RNeasy Micro Kit (spin-column binding capacity of up to 45 µg RNA), the RNeasy Midi Kit (spin-column binding capacity of 1 mg RNA), and RNeasy Maxi Kit (spin-column binding capacity of 6 mg RNA) are also available.

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• RRID-Legacy
• 8 years ago - submitted by Thai Tran

It is nutrient food for feeding mice within quantification of the chemical composition, depends on the purpose of the designed experiments.

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• RRID-Legacy
• 8 years ago - submitted by Thai Tran

The LINCS website is a source of information for the research community and general public about the LINCS project. This website along with the LINCS Data Portal contains details about the assays, cell types, and perturbagens that are currently part of the library, as well as links to participating sites, data releases from the sites, and software that can be used for analyzing the data.

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• RRID-Legacy
• 8 years ago - submitted by Sherry Jenkins

Statistical analyses

• RRID-Legacy
• 8 years ago - submitted by Bo-Chul SHIN

Supplier of algae strains that aims to be a resource to both the HAB research community and biotechnological applications. Researchers can order and deposit strains with the ARC.

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• SciCrunch
• 8 years ago - submitted by Anita Bandrowski

Web server to provide germplasm information about plants, animals, microbes, invertebrates and access to databases that maintain passport, characterization, evaluation, inventory, and distribution data for the management and utilization of national germplasm collections. Under control of the U.S. Department of Agriculture's Agricultural Research Service to support the National Genetic Resources Program (NGRP). Operated by the Database Management Unit of the National Germplasm Resource Laboratory in Beltsville, Maryland.

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• RRID-Legacy
• 8 years ago - submitted by Anita Bandrowski

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• SciCrunch
• 8 years ago - submitted by Ye Peng

R24 OD018250 We are developing an in-depth, easily accessible annotation of the dog genome to support disease research. The domestic dog is an important model for a wide variety of human diseases, including cancer, inflammatory disease, cardiovascular diseases and neurological and behavioral traits. We are using RNA-Seq to generate a more complete gene set and ChIP-seq to map histone marks implicating different regulatory states in different tissues. We are integrating this new data with available SNP data sets, published association signals, and other existing genome annotations to generate a resource easily accessible to the research community. The annotation will be served on the CanFam3.1 genome using the UCSC browser through a Track Hub that we build and manage.

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• SciCrunch
• 8 years ago - submitted by Isabella Froman

2-NBDG (2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose)

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• RRID-Legacy
• 8 years ago - submitted by Alex Tonks

Stay In Kovalam- Patrick Nivas Hotel.

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• SciCrunch
• 8 years ago - submitted by Priyanka Sharma

The Wako HR series NEFA-HR(2) is an in vitro enzymatic colorimetric method assay for the quantitative determination of non-esterified fatty acids (NEFA) in serum.

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• RRID-Legacy
• 8 years ago - submitted by Heike Muenzberg-Gruening

we used it for statics analysis and make graph.

• RRID-Legacy
• 8 years ago - submitted by Kyung-Mi Lee

Dnmt3L Antibody (A-4) is a mouse monoclonal IgG1 (kappa light chain)

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• RRID-Legacy
• 8 years ago - submitted by bing li

Mus musculus

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• SciCrunch
• 8 years ago - submitted by Midori Edamatsu

Rabbit polyclonal antibody to Dopamine beta-Hydroxylase (dopamine beta-hydroxylase (dopamine beta-monooxygenase))

• SciCrunch
• 8 years ago - submitted by Carmen Martínez Ramírez

Mouse Monoclonal Antibody to Glial Fibrillary Acidic Protein clone 2A5, useful as a human GFAP capture reagent in ELISA. Figure shows western blot analysis of equal amount of total protein from different tissue lysates  and recombinant proteins solutions using mouse mAb to GFAP, MCA-2A5, dilution 1:2,000 in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord, [6] pig brain, [7] rat recombinant GFAP, [8] human recombinant GFAP. Bands around 50kDa correspond to alternative transcripts and proteolytic products of GFAP. Note that MCA-2A5 antibody has significantly stronger reactivity with pig and human GFAP as compared to rodent, suggesting that it binds to an epitope which is not totally conserved across mammalian sequences.

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• SciCrunch
• 8 years ago - submitted by Gerry Shaw