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Core provides RNA library preparation services for Illumina, PacBio and Oxford Nanopore sequencing platforms. Single Cell RNA and Spatial Transcriptomics services are available with 10X Genomics technology. Provides RNA extraction, RNA QC and SNP genotyping and methylation bead array services. Provides support from project design through downstream analysis.Service facility, from hypothesis to publication.Microarray: expression and genotyping Affymetrix, Agilent, Nimblegen. Sequencing: RNA, gDNA, ChIP, Capture, 16SNovaSeq, HiSeq 2500, 3000, MiSeq.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Core dedicated to facilitation and development of congenic mice for pre clinical projects across United States. Provides genotyping, background checks to verify purity of mice DNA.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Core Facility provides expertise and advice for experimental design of transgenic or gene knockout experiments, including DNA construct production and genotyping assays, makes reagents available for generation of transgene or gene targeting constructs, and performs all experimental aspects, which include pronuclear injection of transgene constructs, generation of recombinant mouse ES cells, blastocyst injection, and embryo transfer surgeries, for generation or rederivation of genetically manipulated mouse strains. Transgenic founder mice or chimeric animals with targeted alleles are then transferred to individual investigator for analysis.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Provides electron microscopy services for faculty, staff and students of Dartmouth College community, including College, Thayer School of Engineering, Dartmouth Medical School, and Dartmouth-Hitchcock Medical Center. Provides training about how to use instruments and service assistance available for those unable to spend time on microscopes, and for processing of biopsy samples from DHMC.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Core provides murine echocardiography, blood pressure determinations, surgical procedures and phlebotomy, as well as assistance with mouse husbandry and genotyping.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Core offers high throughput screening of large chemical libraries of compounds to identify novel chemical entities that target biological system of interest.Provides target identification and validation, assay development, high throughput screening, hit confirmation, data mining and medicinal chemistry to facilitate hit to lead development.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Research oriented service laboratory providing informatics support to research community. Services include data analysis and mining in proteomics, genomics and chemistry, systems biology approaches such as pathway, network and interaction analyses, large scale statistical and machine learning studies, protein structure, function and stability prediction, sequence and domain analyses,d esign and implementation of relational databases and software programs, consultation on experimental design involving data acquisition, management and analysis, report, grant, and manuscript preparation.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Core provides synthetic chemistry expertise. Includes novel and commercially unavailable small molecules, fluorescent molecules and peptides. Assists in identifying hits for medicinal chemistry optimization in infectious disease targets and provides synthesis capabilities for structure activity studies. Core staff will work with investigators to design and synthesis novel molecular probes. Core provides access to model organism Danio rerio (zebrafish), and allows investigators to image embryonic and adult zebrafish treated with molecular probes for phenotypic drug discovery and other projects, encompasses Purification and Analysis Laboratory that provides purification, analysis and quality control of compounds via HPLC-MS, utilizes automated mass directed fractionation for purification in both reversed and normal phases (including chiral separations), provides relative purity analysis by UPLC coupled to high-resolution mass spectrometer for structure confirmation.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Core focuses on cloning, expression and purification of prokaryotic and eukaryotic proteins for COBRE and other investigators in Kansas and region. Laboratory maintains equipment to support production of properly folded proteins in quantities suitable for structural studies (X-ray and NMR), functional studies (catalytic or biological), label-free binding studies (SPR) and/or high throughput (HTP) screening studies.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Basic research oriented core provides genomic services.Services include Next Generation Sequencing Solutions,DNA and RNA sequencing, Illumina, PacBio, NGS Library preparation including single-cell RNA-seq, Nucleic Acid Extraction Services, total RNA extraction from blood samples in PAXgene tubes, total RNA extractions from cell pellets and miRNA extraction from serum/plasma.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Core provides identification of proteins and metabolites with differential quantitative expression in cells, tissues or in protein affinity purifications. Particular emphasis is on quantitative analysis of posttranslational modifications such as phosphorylation.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Facility consists of a Beckman Coulter ProteomeLab XL-I analytical ultracentrifuge is configured with a scanning UV/Vis detection system and Rayleigh Interference Optics. Used to characterize variety of biophysical properties of macromolecules such as molecular weight, sedimentation coefficient, diffusion coefficient, equilibrium constant and stoichiometry. Can assess sample heterogeneity (aggregation and purity), molecular conformation (folded or unfolded), composition (assembled or unassembled) and thermodynamic properties of interacting systems. Provides spectrum of data for protein characterization in solution.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Core provides equipment with support of research technicians. Research animals are cared for in transient housing rooms. Multiple anesthesia systems and telemetry equipment are available. All procedures are reviewed by Rensselaer Institutional Animal Care and Use Committee (IACUC) and attending veterinarian. Fully outfitted sample and subject preparation workspaces also are available. Additional fees may be incurred for bioresearch animal technician assistance.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Core serves as central molecular biology resource for conception and execution of new projects and provide resources to help offset high costs of developing genetically engineered mice. Ensures better quality control, turn-around time, and cost effectiveness than commercially available services. TACF staff is available for education, assistance and consultation during all stages of project development and production.Serves research staff at West Virginia University, Blanchette Rockefeller Neuroscience Institute and National Institute of Occupational Safety and Health.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Core provides proteomics services to researchers from South Dakota and the surrounding region to rapidly analyze and identify protein expression patterns in their experimental systems.Develops experimental design, protocols, data analysis and interpretation.Provides consulting and advice in grant proposal, as well as data preparation to be submitted to proteomics journal according to requirements.Offers training in use of common equipment such as scanner, spot cutter, imaging software, technique and protocol issues, and sample preparation.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Laboratory performs services in proteomics, genomics and metabolomics by FGCZ personnel without need for user to access labs. Services include Proteomics:Protein identification,quantification,characterization,Glycan/glycoprotein analyses, Analysis of Biomolecules, Fractionation; Genomics:DNA sequencing, RNA sequencing; Metabolomics/Biophysics services.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Core provides flow cytometry instrumentation and analysis. Instruments include Fortessa, FacsAria and Image Stream.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Genomics and transcriptomics core at the UCD Genome Center provides high throughput sequencing, genotyping, single-cell multi-omics and spatial transcriptomics services, training and consultation. Initial consultations are free. The lab uses liquid handling robots to minimize sample handling variation and to provide fast turnaround times.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Facility is equipped withThermoFisher Orbitrap Fusion Lumos Tribrid mass spectrometer that is interfaced to Dionex Ultimate3000 RSLCnano HPLC system. Facility also maintains 2 separate data stations dedicated for proteomics data processing, database searching, and generation of reports.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Facility provides instrumentation and scientific support for single cell analysis and sorting. Routinely performs analysis of both eukaryotic and prokaryotic cells for expression of intracellular and extracellular proteins, cell cycle, cell proliferation, cytokine production, and cell sorting based on expression of cell surface antigen(s) and/or expression of genetically engineered intercellular fluorescent proteins.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth