Curator Dashboard

Provides expertise and equipment to conduct high throughput research. Services and equipment include:Next Generation Sequencing Services,Ion Torrent Proton sequencing,High Throughput Nucleic Acid Manipulation,Qiagen BioSprint 96 high-throughput DNA/RNA extraction system,Eppendorf AF2200 Plate Reader (absorbance and fluorescence based quantification),Lyophilization and Tissue Grinding,PCR Instruments,Two MJ Research 384 well 4 block DNA engine tetrads,Two Applied Biosystems 384 well 2 block Gene Amp PCR,Roche Lightcyler 480 RealTime thermocycler,Miscellaneous Services and Instruments,Blue Pippin and E-gel size selection systems,Agilent Bioanalyzer,Eppendorf epMotion M5073 liquid handling system.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Core services include DNA sequencing, site directed mutagenesis, genome engineering (TALENs & CRISPRS), plasmid preparations and distributing clones/vectors from in house collections. Supports real time quantitative PCR through instrument training, troubleshooting and experimental design. Stowers researchers also have access to Illumina Next Generation Sequencing technology. Constructs libraries, performs sequencing and assists with high throughput genome sequencing, RNA-seq and ChIP-seq projects. Utilizes liquid handling and colony manipulation robots to automate many of services. Provides automation expertise and collaborate with researchers on custom automation projects.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Core offers high field NMR instrumentation for structural studies of biological macromolecules. Instrumentation includes four-channel Bruker Avance 500, 600, and 700 MHz NMR spectrometers, ultra high sensitivity 5mm 1H-13C-15N triple-resonance cold probe for 600 MHz spectrometers. Service include acquisition and analysis of required spectra for elucidation of small molecule structures (includes synthetic molecules, natural products, cofactors, lipids, and short peptides (30 amino acids or less)). Types of projects conducted collaboratively include determination of three-dimensional structures of biological macromolecules, including proteins and nucleic acids, both alone and as complexes with various ligands.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Core provides access to instruments, ranging from simple white light wide field microscopes to fluorescent multidimensional super resolution and confocal imaging systems. Provides expertise in areas of microscopy including laser scanning confocal microscopy, super resolution microscopy, two photon microscopy. Helps with image analysis software packages, including, Imaris, Volocity, Autoquant, MetaMorph, and NIS-Elements.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Provides imaging and quantification instrumentation for analyzing metal quotas at scales ranging from subcellular level to entire ecosystems shaping global biogeochemical cycles. Instruments are capable of quantitatively imaging biologically essential elements in individual cells. Used for research in co-evolution of microbial and eukaryotic life within broad range of challenging chemical environments. Services including instrument training, sample preparation and analysis, experiment design, and grant proposal assistance.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Shared resource designed to produce genetically engineered mice for investigators.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Core provides expertise and resources for large scale biology. Helps to set up, run, gather data and perform analysis in drug discovery research, biochemistry, cell and organismal biology, functional genomic screening, and synthetic genetic. Works with proteins, nucleic acids, small model organisms, and microbial strains. Provides tissue culture,produces and uses lentivirus particles, screens compound libraries, does experiments for investigators,generates preliminary data to figure out if idea is workable, discusses project development. Services include Macromolecular binding, biochemical, and cell-based assays,High content screening with widefield or confocal optics,Nanoliter liquid handling up to 1536-well density,Whole-plate kinetic assays (ion currents, GPCR signaling),Compound library screening,CRISPR/Cas9 screening (multiplexed libraries),Analysis of large data sets,Fluorescence Thermal Shift assay (measures protein melting),Complex liquid handling work flows.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Facility specializes in high resolution chemical imaging by three dimensional atom probe tomography. APT produces three-dimensional (3D) atom-by-atom elementally and isotopically resolved image with sub-nanometer spatial resolution of sample volume typically 100 x 100 x 300 nm^3, by simultaneous high resolution direct-space imaging and atom-by-atom time of flight mass spectrometry. APT is particularly suited to study nano- or nanostructured materials.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Centralized, comprehensive, core laboratory providing histology, immunohistochemistry, molecular analysis and extraction and microscopic evaluation services for human tissue-based studies. Serves integral marker studies that require biomarker-based treatment arm assignment. Performs procurement of fresh biospecimens for clinical trials and biobanking.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Core provides quantitative mass spectrometry based support for in vitro studies and both preclinical and clinical studies of variety of small molecules, including cancer chemotherapeutic agents, analgesics, and antidepressants. Expertise includes optimizing design, conduct, analysis, interpretation, and reportage of pharmacokinetic studies. Helps with biological sample preparation, quantitative mass spectrometric drug concentration measurement, and drug concentration versus time data modeling. Small molecule concentrations in plasma and other body fluids are measured using Sciex 6500 QTrap with UPLC and nano LC or an Agilent HPLC system linked to Applied Biosystems API 3000 triple quadrupole mass spectrometer after sample preparation by, for example, solid-phase extraction. Drug concentration versus time relationships are fitted to various compartmental pharmacokinetic models using commercially available and specialized software.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Shared use and training facility. Servies include Confocal Laser Scanning Microscopy, DIC (Differential Interference Contrast Microscopy), FCS (Fluorescence Correlation Spectroscopy), FLIP (Fluorescence-Loss-In-Photobleaching), FRAP (Fluorescence Recovery After Photobleaching),FRET (Fluorescence/ Forster Resonance Energy Transfer),Live-cell Imaging,Phase Contrast Microscopy,Widefield Fluorescence Microscopy,Image Processing and Analysis. Services include poster printing, and specimen preparation. Provides training for most instruments. Instruments include Leica DM6B Fluorescent Microscope (Hogan 5-112), Leica TCS SP8 Confocal Microscope (Hogan 5-128),Leica SP5 II Confocal Microscope (Hogan 5-114),Leica Spinning Disk Confocal Microscope (Hogan 5-113),DeltaVision Deconvolution Microscope (Hogan 5-111),Olympus IX83 Inverted Fluorescent Microscope (Silverman Hall 1-567),Olympus IX53 Inverted Color Microscope (Silverman Hall 1-567), LionHeart Automated Microscope BioTeck (Hogan 5-110).

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Provides 6 cell sorters and 5 benchtop analyzers. Helps investigators to define their projects in the early stages of development to make optimal and efficient use of flow cytometry. Educates ALL users (faculty and staff) in the science and technology of flow cytometry.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Facility dedicated to help to determine behavioral effects of genetic manipulations, potential pharmaceuticals, aging, and other manipulations upon normal behavior, and learning and memory capacities of rodents used as model systems. Provides mouse and rat stereotaxic surgery, helps design behavioral studies.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Parcellation of adult human brain in 3D, labeling every voxel with brain structure spanning 141 structures. These parcellations were drawn and adapted from prior 2D version of adult human brain atlas.

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• SciCrunch
• 7 years ago - submitted by Anita Bandrowski

Core provides electron microscopy imaging and analytical characterization, surface analysis by XPS or Raman, access to instrumentation, training, and service for using scanning (SEM), transmission (TEM) and scanning transmission (STEM) electron microscopy, surface analysis and vibrational spectroscopy, specimen preparation.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Core provides access to computing infrastructure while offering technical support. Provide tools and instruction allowing for inclusion of high performance computing in classroom.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Core offers mass spectral proteomic analysis. Assists with qualitative and quantitative characterization of proteins in biological matrices such as plasma/serum, tissue, cell lines and other biological material to gain understanding of physiological pathways, molecular interactions and regulatory signaling.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Core offers services in cytometry and antibody technology, helps to design projects, data acquisition, analysis and/or interpretation beyond routine practices,critical drafting and/or revision of manuscript for intellectual content purposes.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Core provides sequencing resources. Service support for Illumina Next Generation and Sanger sequencing projects, from project design and sample preparation through downstream analysis. Provides DNA extraction, SNP genotyping, and fragment analysis services, and can facilitate custom projects upon request.

• Resource
• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Core to assist researchers and students in their imaging needs of fluorescence and electron microscopy and to increase use of microscopes in science education. Consultation, training or touring facility is also available by appointment.

• Resource
• SciCrunch
• 7 years ago - submitted by Edyta Vieth