Curator Dashboard

Core provides analysis of proteins, peptides, lipids, polar metabolites and small molecules by high resolution/high mass spectrometry. Targeted and hypothesis free analysis are offered combined with relative quantitation based on either label free, tandem mass tags or metabolic labelling. Acquisition tools include Data Dependent (DDA) and Data Independent (DIA).

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Core provides consulting and services in X-ray Photoelectron Spectroscopy (XPS or ESCA), Time-of-Flight Secondary-Ion Mass Spectrometry (TOF-SIMS), X-ray Photoelectron Imaging, Auger Electron Spectroscopy (AES), Scanning Auger Microscopy or Imaging (SAM), Secondary-Electron Microscopy (SEM), Ion-Scattering Spectroscopy (ISS or LEIS), Scanning Probe Microscopy (STM and AFM).

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Core produces fully human, full length, antigen specific antibodies for use in studying human immune responses. Produces high affinity protective antibodies to influenza, anthrax lethal toxin, and various S. pneumonia polysaccharides. Helps quantify antibody secreting cell responses after vaccination and by generating human monoclonal antibodies to be characterized. Pathogen specific human monoclonal antibodies are also available for licensing agreements and other forms of commercial development. Services include to define temporal human immune responses after vaccination and to generate human monoclonal antibodies to supplement research,development of novel methods to produce human monoclonal antibodies after acute infection and other immune events, investigation of new technologies for characterization of human monoclonal antibodies produced by core.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Core provides fully automated high throughput screening (HTS) of Compound Libraries (130,000+ compounds) for both enzyme/protein-based assays and cell-based assays, using Caliper Life Sciences Staccato system;Genomic siRNA screening with siARRAY whole human genome siRNA library from Dharmacon targeting 21,000 genes, using Agilent Bravo system;High-Content Screening using ImageXpress Micro automated fluorescent microscope with live cell, bright field, phase contrast and integrated plate handling with Thermo Catalyst CRS, and image analysis using MetaXpress software;High Throughput Molecular Biology reagents and services, including access to cDNA libraries (Human ORFeome collection, 15,000 genes) and 96 and 384-well bead clean-ups and PCR setup (Biomek FX and Agilent Bravo), and other automation steps in collaboration with SFGF;High-throughput assay development assistance with cell culture, experiment design, robotic programming and Standard Operating Procedure drafting;Screening data analysis assistance with protocols, hit determination and structure activity analyses using MDL chemical database ISIS/HOST, Plate Manager, Assay Explorer and Report Manager. Use of microplate reader detection systems, including Tecan Infinite M1000 and Infinite M1000 PRO and Molecular Devices Analyst GT for fluorescence; fluorescence polarization; time-resolved fluorescence; absorbance and luminescence (with injectors and AlphaScreen); and Flexstation II 384, for kinetic fluorescence reads to measure calcium mobilization and ion channels.Use of liquid-handling robots, including Sciclone ALH3000 (96- and 384-well pipetting), Agilent Bravo (96- and 384-well pipetting), Velocity11 VPrep (96-well pipetting), Bio-Tek plate washers/dispensers, Matrix Wellmate and Titertek/Labsystems Multidrop microplate dispensers, and Velocity11 PlateLoc plate heat sealer;Training for most of these services.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Facility is equipped with high performance computers, bioinformatics related software, biological databases, and data storage. Provides bioinformatics and computational services in virology, molecular biology, genomics, proteomics, next generation high throughput sequencing, and other biological related fields. For special requirements, can design and develop new algorithms, and implement programs and customize data processing pipelines. Core promotes education in bioinformatics to students and researchers, develops and delivers training material, including workshops that facilitate practical application of bioinformatics knowledge by students, postdoctoral fellows, and faculties in NCV community.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Provides support to University researchers for transmission- and scanning-electron microscopy. Based on center capacity, support is also available to researchers from other not-for-profit institutions.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Provide members of University and their visitors with wide spectrum of optical microscopy equipment and extensive training in its use.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Provides help with grant review, information on data management plans,suggestion best practices for bionformatics analyses, advise on experimental design for Next Gen Sequencing (NGS) projects.Services include:Genome assembly and annotation,Transcriptome assembly and annotation,SNP/InDel calling,RNA-Seq analysis,ChiP-seq,Introgression mapping,novel gene discovery,Personalized GBrowse instances for data visualization,Access to high performance computing, Custom big data projects.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Provides tools of modern functional proteomics and metabolomics. Facility is equipped with chromatography and mass spectrometry based technologies for proteomics and metabolomics (Clinical and non clinical), personalized experimental design consultation and comprehensive, individualized bioinformatics support.Services include:Small molecule exact mass determination or quantitation using positive or negative ion mode;Protein identification using LC/MS/MS analysis and MASCOT and SEQUEST database search;Shot gun proteome analysis of biological samples;Biomarker discovery from biological fluid;Drug protein or drug nucleic acid protein interaction;Protein complex isolation and identifying interacting partners and its quantitation;Protein differential expression analysis and quantitation by 2D-LC MS/MS (MudPIT);Global PTM analysis and quantitation;Specialization in phosphorylation and oxidation analysis;Coomassie Blue and Silver Stained Gel analysis; de novo peptide sequencing by tandem mass spectrometry;Confirmation of mutations in protein ;Customized sequence search of in-house proteins that are not available in database; Post translational modifications (phosphorylation, sumoylation, ubiquitination, oxidation, etc.); Determination of oxidation state of cysteine (disulfide bonds);Intact proteins and peptides mass determination.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Provides flow cytometry instrumentation and expertise. Provides operator assisted analyzer and sorter use, as well as training and support for user instrument operation.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Provides high resolution, light and electron microscopy technologies for imaging and analyzing molecular and structural organization of cells, tissue and bioengineered materials. Operates two sites at Stanford University: SOM Beckman Center CSIF and SOE Shriram Center CSIF.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Provides University of Massachusetts Medical School investigators with clinical research support in biostatistics, experimental design, and data management.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Provides system for Splicing isoform Annotation. This LISA platform allows high throughput annotation and functional analysis of Alternate Splicing in humans.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Core sequencing services include Illumina Sequencers, Single-Cell RNA-Seq,Single-Cell Immune Profiling,Single-Cell ATAC-Seq,Linked-Read Sequencing. Provides bioinformatic analysis including Single-Cell RNA Analysis,Linked-Read Genome Analysis,ChIP-Seq Analysis Package,RNA-Seq Analysis Package,miRNA Analysis Package,Custom Project Requests,A La Carte Bioinformatics. Provides services including QX100 Droplet Digital PCR Machine (Biorad),Nucleic acid extractions from bio-specimens, Sample QC,Realtime PCR.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Facility operates as full service facility delivering transgenics and cell cultures to researchers and self service facility providing high quality space for researchers to use for their own specific project needs. Services available include transformations and cell/ tissue culture in many different plant species, project consultation, training workshops, self-service hood usage and offering high quality growth space. Facility staff has developed portfolio of transformation and tissue culture systems such as: Setaria, tobacco, petunia, tomato, Arabidopsis, soybean, Indian mustard, maize, rice and various different cell cultures. Within the facility, researchers have also produced cassava, sweet potato, and potato. Transformation systems will be added, developed, inherited, or modified as needed.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Core provides analysis of gene expression using Affymetrix GeneChip platform. Offers next generation sequencing via Illumina NextSeq and Ion Torrent PGM and Proton instruments, analysis of RNA and DNA using Agilent Bioanalyzer.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Core services include Custom DNA Sequencing (3730xl for dideoxy sequencing chemistry, or Illumina HiSeq 2500 for large scale sequencing projects),DNA Fragment Analysis,Sequencing and PCR troubleshooting and training,SNP analysis,Primer walking, Contig assembly and editing.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Core offers protein/peptide sequencing, large and small scale peptide synthesis (Fmoc), matrix assisted laser desorption/ionization (MALDI) mass spectrometry, SDS-PAGE/electroblotting, 2-D gel electrophoresis, isoelectric focusing (IEF), in-gel and solution digestion, tandem mass spectrometry (LC-MS/MS), ion mobility mass spectrometry (IM-MS), digital image acquisition and analysis using Typhoon imaging system and 2D gel documentation/analysis system, and semi-preparative, analytical and micro-analytical high performance liquid chromatography (HPLC). MALDI, HPLC, SDS-PAGE/blotting, IEF, 2D gel electrophoresis, 2D gel documentation/analysis and Typhoon imaging system are all available for user operation after appropriate training.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Central micro/nano fabrication facility of Northwestern University. Provides range of nanofabrication equipment and technical expertise. Supports research in all areas of science, engineering, medicine, and interdisciplinary fields.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Core research facility providing services in high-throughput DNA sequencing that includes staff experience in the construction of libraries for targeted amplicons, miRNA analysis, transcriptome profiling (RNA-Seq), whole exome sequencing (WES), epigenomic and genomic DNA analysis. Services in single cell library preparation are available using the 10x Genomics Chromium X system. In addition, spatial transcriptomics services using Visium HD arrays are provided. The GTC maintain and operate Illumina sequencing platforms; NovaSeq X Plus and MiSeq i100 instruments.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth