Curator Dashboard

Core provides analytical and preparative flow cytometers, to facilitate range of experiments in biology of both normal and cancer cells. Experimental analyses, including:Complex, 19 fluorescence parameter immuno-phenotyping;DNA content, ploidy and cell cycle analysis; 17 fluorescence parameter cell sorting and single-cell flow cloning; High speed (10,000 � 30,000+ cells per second) and efficiency (95%+ purity) cell sorting;Assessment of apoptosis;Molecular cytogenetics;Cell function studies;Micro-biological applications;Multiplexed and micro-particle-based analysis;Mass cytometry analysis and imaging;Fluidigm Helios plasma mass cytometer. Cells are labeled with antibodies conjugated to heavy metal markers, vaporized in plasma and detected with time-of-flight mass spectrometry;Fluidigm Hyperion solid tissue imager provides simultaneous imaging of labeled solid tissue sections, for mass cytometry analysis. FCSR has bank of metal reagent kits (for custom antibody conjugation), available for purchase at favorable rates compared to those available commercially. The bank has 35 unique labeling metals, for users to create custom reagents and panels. Please contact FCSR for further information and pricing. Robotic automated cell transfer A.L.S. CellCelector automated cell transfer robot. For automated transfer of single cells and colonies from culture plates, without pre-treatment. Integral bio-safety cabinet for BSL-2 cultures.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Core provides services and houses samples collected over the last 30 years. Biorepository processes, archives, and retrieves biological samples for genomic research. Offers variety of sample processing options including but not limited to automated DNA extraction using Autogen FLEXSTAR+ instrument, automated DNA/RNA extraction using Qiagen QIASymphony, peripheral blood mononuclear cell (PBMC) isolation, serum, plasma and buffy coat isolation, creation of blood cards, DNA/RNA quantitation and qualitation, whole genome amplification, cell-line lymphoblast immortalization and primary fibroblast tissue culture. Offers sample solutions tos uit customized needs.Services also include DNA/RNA Extraction,Sample archiving, retrieval and allocation,Unique and custom labels printing,DNA/RNA quantitation and qualitation,Tissue culture,Whole genome amplification.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Core provides tools for preparation of human and murine samples suitable for molecular genetic and cytogenetic analysis of entire genome. These tools include establishment of EBV transformed cell lines; isolation of DNA and mRNA from variety of tissue culture samples as well as primary biopsies; preparation of metaphase chromosomes suitable for fluorescence in situ hybridization (FISH) and Spectral Karyotyping (SKY) or whole chromosome paints for human and mouse genome. Core personnel is trained to hybridize commercial probes and to designed locus specific probes for regions of interest to investigators. All probes are custom designed and in house generated.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Multi-user microscopy facility that houses state-of-the-art equipment ranging from confocal microscopes to super-resolution microscopes to the electron and atomic force microscopes, enabling to acquire information from the millimeter to nanometer scales. Staff has technical expertise across microscopy platforms and methodologies with a special emphasis on life sciences and soft materials.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Core provides seven mass spectrometry platforms, for shotgun and targeted analyses, run by core staff. Services include protein and peptide identification from gels and solutions, identification and localization of post translational modifications, relative and absolute quantitation of peptides and proteins, intact mass analysis of proteins in solution.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Core provides researchers with ability to visualize samples, from monolayers and small organisms, such as developing fly and fish embryos, to very thick sections from brain and other organ tissues by using instruments including laser point (LSCM) and field scanning confocal (CSU), Total Internal Reflectance Fluorescence (TIRF), Multi Photon (MP), and Widefield (WF). Services in imaging in mammalian cells, yeast cells, Drosophila and Zebrafish embryos and ovaries, bacteria, sections of brain and other tissues, in both fixed and live specimens, Quantitative imaging methods such as FRAP, FLIP, and FRET, software packages for image processing, analysis, and 3D image reconstruction.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Core facility that supports expression, purification, and analysis of reagent and preclinical proteins by providing instrumentation and consultation for protein production from small to large scale. Available equipment includes shaking incubators, 5 L fermentor, high pressure cell disruptor, hollow fiber concentrator, Maxwell16 magnetic bead purification system, AKTA Purifier chromatograph, analytical ultracentrifuge, analytical HPLC, and MALDI-QIT-TOF mass spectrometer.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Core provides two experimental 7 Tesla magnetic resonance imaging machines which were purchased by Center for Stroke Research CSB (Device 1) and Cluster of Excellence NeuroCure (Device 2) with aim of creating experimental platform for small animal imaging. Services projects in areas of experimental neuroimaging and molecular imaging, but projects in other fields of research are explicitly encouraged.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Core provides complete CRISPR services, design, production of gRNAs & Cas9 mRNA, and injection.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Core provides instrumentation in area of solution phase, high resolution NMR spectroscopy and expertise. Primary solution state spectroscopy of bio molecules including, but not limited to, studies of polymers, minerals, soils, and extracts. Equipment includes: 800MHz Varian Inova Spectrometer, 600MHz Varian Inova Spectrometer, 500MHz Varian Inova Spectrometer, and staff assistance/consultation is available.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Core provides flow cytometry services to scientists, offers analytical cytometry and high-speed sorting either in investigator operated format or facility staff assisted format, offers training for research personnel interested in learning how to design flow cytometry experiments, operate instrumentation and analyze data. Services include experimental design, protocol development, sample staining, instrument operation, data analysis, graphical production, sample processing as a Service (SpaaS). Facility is equipped with three cell sorters and four cell analyzers.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Core provides mass spectrometric services, from basic mass measurement to complex proteome analyses.Services include basic mass measurement, ESI-MS/MS,LC-MS,Exact mass measurement,Protein identification.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Core using ultrahigh performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) offers high-throughput analytical services for determining diagnostic amino acids and their precursors or metabolic products. Available biomarkers include citruilline and tetrahydrobiopterin. Determination of other small molecules is possible.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Services include advise on microscope to use for research project, image acquisition, optimization image analysis and sample preparation, free of charge access to 500GB per laboratory on CMCI server in order to store data for project. Facility houses four wide-field, near-field (TIRF), three confocal (swept-field and laser-scanning) microscopes and laser capture microdissection system.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Provides services in sequencing using ABI model 3730 DNA Sequencer with the XL Upgrade,design and have synthesized primers for primer walking experiments, DNA fragment analysis such as microsatellites, AFLP, SNP analysis.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Provides bioinformatics research resources, services and training in support of existing research programs and in development of new programs and grant applications. Provides next-generation sequencing data analysis including RNA-Seq and QuantSeq analysis, de novo transcriptome assembly, whole-genome sequencing, genome resequencing, single-cell sequencing, metagenomics, microbiome, mass spectrometry proteomic analysis of label-free (DIA) and TMT data, analysis of metabolomics, microbiome, multi-omics data, data mining, modeling, and visualization. Provides customized bioinformatics and statistical services including data quality control, power studies, hypothesis tests, regression, clustering, network analysis, function annotation, pathway analysis including iPathwayGuide.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Core mass spec and proteomic services include open access lab for trained users with GC/MS, LC/MS, high resolution LC/MS, and MALDI-TOF instruments, help with intact protein analysis, targeted quantitation, drug discovery support, pathway analysis, protein interactions, FFPE tissue analysis, both labeled and label-free proteomics, and more. Please contact SUMS to discuss these and other custom projects including new application development.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Core assists with epigenetic analysis including DNA Methylation, Chromatin Immunoprecipitation and Real Time Quantitative PCR gene expression analysis. Services include DNA Methylation Analysis, Specific Genomic Location Analysis:Methylation Specific PCR,Bisulfite Sequencing,Bisulfite Pyrosequencing,Qiagen PyroMark Pyrosequencer Instrumentation;Genome Wide Analysis:High Throughput Sequencing Methylation Analysis, Methyl-Sensitive Cut Counting (MSCC),Methyl CpG Binding Domain - Isolated Genome Sequencing (MiGS);Chromatin Immunoprecipitation Analysis (ChIP):Analysis of Histone Modifications,DNA-Protein Interactions,Chromatin Positions analyzed using:Quantitative PCR Analysis (Real-Time QPCR),High Throughput Sequencing Analysis (ChIP-Seq);Gene Expression Analysis (QPCR):Real-Time Quantitative PCR Gene Expression.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Facility within UW Biotechnology Center (UWBC) for assisting researchers with their data analysis needs. Helps with variety of common sequencing related projects, DNA-seq, ChIP-Seq, RNA-Seq, 16S metagenomics, and small RNA analyses.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Core provides imaging systems including light/fluorescence microscopes, confocal laser scanning microscopes and electron microscopes.Provides services, teaching, training and technical support for multi disciplinary research.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth