Curator Dashboard

Services include advise on microscope to use for research project, image acquisition, optimization image analysis and sample preparation, free of charge access to 500GB per laboratory on CMCI server in order to store data for project. Facility houses four wide-field, near-field (TIRF), three confocal (swept-field and laser-scanning) microscopes and laser capture microdissection system.

• Resource
• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Provides services in sequencing using ABI model 3730 DNA Sequencer with the XL Upgrade,design and have synthesized primers for primer walking experiments, DNA fragment analysis such as microsatellites, AFLP, SNP analysis.

• Resource
• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Provides bioinformatics research resources, services and training in support of existing research programs and in development of new programs and grant applications. Provides next-generation sequencing data analysis including RNA-Seq and QuantSeq analysis, de novo transcriptome assembly, whole-genome sequencing, genome resequencing, single-cell sequencing, metagenomics, microbiome, mass spectrometry proteomic analysis of label-free (DIA) and TMT data, analysis of metabolomics, microbiome, multi-omics data, data mining, modeling, and visualization. Provides customized bioinformatics and statistical services including data quality control, power studies, hypothesis tests, regression, clustering, network analysis, function annotation, pathway analysis including iPathwayGuide.

• Resource
• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Core mass spec and proteomic services include open access lab for trained users with GC/MS, LC/MS, high resolution LC/MS, and MALDI-TOF instruments, help with intact protein analysis, targeted quantitation, drug discovery support, pathway analysis, protein interactions, FFPE tissue analysis, both labeled and label-free proteomics, and more. Please contact SUMS to discuss these and other custom projects including new application development.

• Resource
• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Core assists with epigenetic analysis including DNA Methylation, Chromatin Immunoprecipitation and Real Time Quantitative PCR gene expression analysis. Services include DNA Methylation Analysis, Specific Genomic Location Analysis:Methylation Specific PCR,Bisulfite Sequencing,Bisulfite Pyrosequencing,Qiagen PyroMark Pyrosequencer Instrumentation;Genome Wide Analysis:High Throughput Sequencing Methylation Analysis, Methyl-Sensitive Cut Counting (MSCC),Methyl CpG Binding Domain - Isolated Genome Sequencing (MiGS);Chromatin Immunoprecipitation Analysis (ChIP):Analysis of Histone Modifications,DNA-Protein Interactions,Chromatin Positions analyzed using:Quantitative PCR Analysis (Real-Time QPCR),High Throughput Sequencing Analysis (ChIP-Seq);Gene Expression Analysis (QPCR):Real-Time Quantitative PCR Gene Expression.

• Resource
• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Facility within UW Biotechnology Center (UWBC) for assisting researchers with their data analysis needs. Helps with variety of common sequencing related projects, DNA-seq, ChIP-Seq, RNA-Seq, 16S metagenomics, and small RNA analyses.

• Resource
• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Core provides imaging systems including light/fluorescence microscopes, confocal laser scanning microscopes and electron microscopes.Provides services, teaching, training and technical support for multi disciplinary research.

• Resource
• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Core provides analysis of proteins, peptides, lipids, polar metabolites and small molecules by high resolution/high mass spectrometry. Targeted and hypothesis free analysis are offered combined with relative quantitation based on either label free, tandem mass tags or metabolic labelling. Acquisition tools include Data Dependent (DDA) and Data Independent (DIA).

• Resource
• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Core provides consulting and services in X-ray Photoelectron Spectroscopy (XPS or ESCA), Time-of-Flight Secondary-Ion Mass Spectrometry (TOF-SIMS), X-ray Photoelectron Imaging, Auger Electron Spectroscopy (AES), Scanning Auger Microscopy or Imaging (SAM), Secondary-Electron Microscopy (SEM), Ion-Scattering Spectroscopy (ISS or LEIS), Scanning Probe Microscopy (STM and AFM).

• Resource
• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Core produces fully human, full length, antigen specific antibodies for use in studying human immune responses. Produces high affinity protective antibodies to influenza, anthrax lethal toxin, and various S. pneumonia polysaccharides. Helps quantify antibody secreting cell responses after vaccination and by generating human monoclonal antibodies to be characterized. Pathogen specific human monoclonal antibodies are also available for licensing agreements and other forms of commercial development. Services include to define temporal human immune responses after vaccination and to generate human monoclonal antibodies to supplement research,development of novel methods to produce human monoclonal antibodies after acute infection and other immune events, investigation of new technologies for characterization of human monoclonal antibodies produced by core.

• Resource
• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Core provides fully automated high throughput screening (HTS) of Compound Libraries (130,000+ compounds) for both enzyme/protein-based assays and cell-based assays, using Caliper Life Sciences Staccato system;Genomic siRNA screening with siARRAY whole human genome siRNA library from Dharmacon targeting 21,000 genes, using Agilent Bravo system;High-Content Screening using ImageXpress Micro automated fluorescent microscope with live cell, bright field, phase contrast and integrated plate handling with Thermo Catalyst CRS, and image analysis using MetaXpress software;High Throughput Molecular Biology reagents and services, including access to cDNA libraries (Human ORFeome collection, 15,000 genes) and 96 and 384-well bead clean-ups and PCR setup (Biomek FX and Agilent Bravo), and other automation steps in collaboration with SFGF;High-throughput assay development assistance with cell culture, experiment design, robotic programming and Standard Operating Procedure drafting;Screening data analysis assistance with protocols, hit determination and structure activity analyses using MDL chemical database ISIS/HOST, Plate Manager, Assay Explorer and Report Manager. Use of microplate reader detection systems, including Tecan Infinite M1000 and Infinite M1000 PRO and Molecular Devices Analyst GT for fluorescence; fluorescence polarization; time-resolved fluorescence; absorbance and luminescence (with injectors and AlphaScreen); and Flexstation II 384, for kinetic fluorescence reads to measure calcium mobilization and ion channels.Use of liquid-handling robots, including Sciclone ALH3000 (96- and 384-well pipetting), Agilent Bravo (96- and 384-well pipetting), Velocity11 VPrep (96-well pipetting), Bio-Tek plate washers/dispensers, Matrix Wellmate and Titertek/Labsystems Multidrop microplate dispensers, and Velocity11 PlateLoc plate heat sealer;Training for most of these services.

• Resource
• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Facility is equipped with high performance computers, bioinformatics related software, biological databases, and data storage. Provides bioinformatics and computational services in virology, molecular biology, genomics, proteomics, next generation high throughput sequencing, and other biological related fields. For special requirements, can design and develop new algorithms, and implement programs and customize data processing pipelines. Core promotes education in bioinformatics to students and researchers, develops and delivers training material, including workshops that facilitate practical application of bioinformatics knowledge by students, postdoctoral fellows, and faculties in NCV community.

• Resource
• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Provides support to University researchers for transmission- and scanning-electron microscopy. Based on center capacity, support is also available to researchers from other not-for-profit institutions.

• Resource
• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Provide members of University and their visitors with wide spectrum of optical microscopy equipment and extensive training in its use.

• Resource
• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Provides help with grant review, information on data management plans,suggestion best practices for bionformatics analyses, advise on experimental design for Next Gen Sequencing (NGS) projects.Services include:Genome assembly and annotation,Transcriptome assembly and annotation,SNP/InDel calling,RNA-Seq analysis,ChiP-seq,Introgression mapping,novel gene discovery,Personalized GBrowse instances for data visualization,Access to high performance computing, Custom big data projects.

• Resource
• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Provides tools of modern functional proteomics and metabolomics. Facility is equipped with chromatography and mass spectrometry based technologies for proteomics and metabolomics (Clinical and non clinical), personalized experimental design consultation and comprehensive, individualized bioinformatics support.Services include:Small molecule exact mass determination or quantitation using positive or negative ion mode;Protein identification using LC/MS/MS analysis and MASCOT and SEQUEST database search;Shot gun proteome analysis of biological samples;Biomarker discovery from biological fluid;Drug protein or drug nucleic acid protein interaction;Protein complex isolation and identifying interacting partners and its quantitation;Protein differential expression analysis and quantitation by 2D-LC MS/MS (MudPIT);Global PTM analysis and quantitation;Specialization in phosphorylation and oxidation analysis;Coomassie Blue and Silver Stained Gel analysis; de novo peptide sequencing by tandem mass spectrometry;Confirmation of mutations in protein ;Customized sequence search of in-house proteins that are not available in database; Post translational modifications (phosphorylation, sumoylation, ubiquitination, oxidation, etc.); Determination of oxidation state of cysteine (disulfide bonds);Intact proteins and peptides mass determination.

• Resource
• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Provides flow cytometry instrumentation and expertise. Provides operator assisted analyzer and sorter use, as well as training and support for user instrument operation.

• Resource
• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Provides high resolution, light and electron microscopy technologies for imaging and analyzing molecular and structural organization of cells, tissue and bioengineered materials. Operates two sites at Stanford University: SOM Beckman Center CSIF and SOE Shriram Center CSIF.

• Resource
• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Provides University of Massachusetts Medical School investigators with clinical research support in biostatistics, experimental design, and data management.

• Resource
• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Provides system for Splicing isoform Annotation. This LISA platform allows high throughput annotation and functional analysis of Alternate Splicing in humans.

• Resource
• SciCrunch
• 7 years ago - submitted by Edyta Vieth