Curator Dashboard

Core provides upgraded Bruker SMART 1000 CCD single crystal diffractometer to SMART APEX II CCD Area Detector, and its low-temperature LT3 device to KRYO-FLEX low-temperature device. Instrument which is equipped with Mo radiation source is used for small molecule crystal structure determination.

• Resource
• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Facility provides imaging methods and instruments, microscopes that reveal biological processes in three-dimensional detail and behavior of single molecules in high resolution. Welcomes proposals with high-risk/high-gain projects. AIC will cover lodging and basic experimental cost for scientists whose proposals have been accepted to come to Janelia to perform their experiments.

• Resource
• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Full service veterinary reference laboratory directed by members of faculty of University of Miami Miller School of Medicine. Offers clinical pathology and histopathology services to meet needs of avian, exotic, lab animal, and wildlife veterinarians. Services include Acute Phase Protein Laboratory. Provides recent diagnostic testing developments which may not yet be available in other veterinary diagnostic testing environments.

• Resource
• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Core facility provides neural stem cells (NSC) and everything you need to grow them, custom lentiviral shRNA and over-expression vectors, consult based characterization service where we can assess how your products or reagents affect stem cell performance and behavior. Services include:Cell Supply,Adult SVZ Neural Stem Cells,Any Age Embryonic stem cells from any region of the brain,Human Retinal Pigmented Epithelial cells,RPE iPSCs,iPSCs,Neural Progenitor Cells (NPCs),Custom Orders,Media/ Dissociation Reagents,High-performance Media formulated according to optimal cell growth conditions,Complete Defined Media for Neural Stem Cell Culture,Complete Defined Media for Human Retinal Pigmented Epithelial Culture,Cell characterization. Performs assays to verify how stem cell populations behave in response to certain environments, plastics, reagents, etc., using trade secret assays via FACS and cell culture techniques. This can be used for neural stem cells as well as embryonic stem cells.Cell banking solutions shall ensure that their integrity is maintained, and that sufficient supply is readily available. We will also expand cells, harvest cells, generation the freezing medium and thaw cells to test performance. Provides Generation of custom lentiviral shRNA constructs,Generation of custom lentiviral over-expression constructs,Supply of NSC lines already expressing these shRNA or over-expression constructs.

• Resource
• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Core provides services in analyzing genetic variation, performing genome-wide association studies, conducting non-human genotyping. Offers flexible platforms from low to high throughput, tailored to research needs. Specializes in Illumina Genotyping Arrays and TaqMan Allelic Discrimination Assays, with fully automated processing using Tecan EVOs and BioMek liquid handlers and Genologics LIMS for reliable data tracking.Applications include: GWAS – Identify genetic variants linked to traits and diseases; PRS Analysis – Assess genetic risk for complex diseases; Pharmacogenomics – Study genetic factors in drug response; Population Genetics – Explore ancestry and genetic diversity; CNV Analysis – Detect large-scale genomic variations; Methylation Analysis.

• Resource
• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Core provides two mass spectrometers at UM Department of Chemistry, Bruker MicroQ-TOF ESI, and Agilent Technologies 5975C GC/MS with autosampler.

• Resource
• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Core provides access to mass spectrometry based proteomics applications, customizes mass spectrometry designs that fit individual needs. Used for general proteomics evaluations and protein identification designs, quantitation techniques, hydrogen/deuterium exchange, and oxidative footprinting. Equipment includes Orbitrap Fusion Lumos.

• Resource
• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Core provides analytical and preparative flow cytometers, to facilitate range of experiments in biology of both normal and cancer cells. Experimental analyses, including:Complex, 19 fluorescence parameter immuno-phenotyping;DNA content, ploidy and cell cycle analysis; 17 fluorescence parameter cell sorting and single-cell flow cloning; High speed (10,000 � 30,000+ cells per second) and efficiency (95%+ purity) cell sorting;Assessment of apoptosis;Molecular cytogenetics;Cell function studies;Micro-biological applications;Multiplexed and micro-particle-based analysis;Mass cytometry analysis and imaging;Fluidigm Helios plasma mass cytometer. Cells are labeled with antibodies conjugated to heavy metal markers, vaporized in plasma and detected with time-of-flight mass spectrometry;Fluidigm Hyperion solid tissue imager provides simultaneous imaging of labeled solid tissue sections, for mass cytometry analysis. FCSR has bank of metal reagent kits (for custom antibody conjugation), available for purchase at favorable rates compared to those available commercially. The bank has 35 unique labeling metals, for users to create custom reagents and panels. Please contact FCSR for further information and pricing. Robotic automated cell transfer A.L.S. CellCelector automated cell transfer robot. For automated transfer of single cells and colonies from culture plates, without pre-treatment. Integral bio-safety cabinet for BSL-2 cultures.

• Resource
• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Core provides services and houses samples collected over the last 30 years. Biorepository processes, archives, and retrieves biological samples for genomic research. Offers variety of sample processing options including but not limited to automated DNA extraction using Autogen FLEXSTAR+ instrument, automated DNA/RNA extraction using Qiagen QIASymphony, peripheral blood mononuclear cell (PBMC) isolation, serum, plasma and buffy coat isolation, creation of blood cards, DNA/RNA quantitation and qualitation, whole genome amplification, cell-line lymphoblast immortalization and primary fibroblast tissue culture. Offers sample solutions tos uit customized needs.Services also include DNA/RNA Extraction,Sample archiving, retrieval and allocation,Unique and custom labels printing,DNA/RNA quantitation and qualitation,Tissue culture,Whole genome amplification.

• Resource
• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Core provides tools for preparation of human and murine samples suitable for molecular genetic and cytogenetic analysis of entire genome. These tools include establishment of EBV transformed cell lines; isolation of DNA and mRNA from variety of tissue culture samples as well as primary biopsies; preparation of metaphase chromosomes suitable for fluorescence in situ hybridization (FISH) and Spectral Karyotyping (SKY) or whole chromosome paints for human and mouse genome. Core personnel is trained to hybridize commercial probes and to designed locus specific probes for regions of interest to investigators. All probes are custom designed and in house generated.

• Resource
• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Microscopy facility that houses equipment including confocal microscopes: LSM780 confocal microscope (Located at CBBI),LSM880 confocal microscope (Located at DBI 117),electron microscopes and their accessory instrumentation:Thermo Scientific Apreo VS SEM microscope,Hitachi S-4700, Leica EM ACE600 and Tousimis Autosamdri-815B,CX7 high content analysis system. Our staff has technical expertise across different microscopy platforms and methodologies.

• Resource
• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Core provides seven mass spectrometry platforms, for shotgun and targeted analyses, run by core staff. Services include protein and peptide identification from gels and solutions, identification and localization of post translational modifications, relative and absolute quantitation of peptides and proteins, intact mass analysis of proteins in solution.

• Resource
• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Core provides researchers with ability to visualize samples, from monolayers and small organisms, such as developing fly and fish embryos, to very thick sections from brain and other organ tissues by using instruments including laser point (LSCM) and field scanning confocal (CSU), Total Internal Reflectance Fluorescence (TIRF), Multi Photon (MP), and Widefield (WF). Services in imaging in mammalian cells, yeast cells, Drosophila and Zebrafish embryos and ovaries, bacteria, sections of brain and other tissues, in both fixed and live specimens, Quantitative imaging methods such as FRAP, FLIP, and FRET, software packages for image processing, analysis, and 3D image reconstruction.

• Resource
• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Core facility that supports expression, purification, and analysis of reagent and preclinical proteins by providing instrumentation and consultation for protein production from small to large scale. Available equipment includes shaking incubators, 5 L fermentor, high pressure cell disruptor, hollow fiber concentrator, Maxwell16 magnetic bead purification system, AKTA Purifier chromatograph, analytical ultracentrifuge, analytical HPLC, and MALDI-QIT-TOF mass spectrometer.

• Resource
• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Core provides two experimental 7 Tesla magnetic resonance imaging machines which were purchased by Center for Stroke Research CSB (Device 1) and Cluster of Excellence NeuroCure (Device 2) with aim of creating experimental platform for small animal imaging. Services projects in areas of experimental neuroimaging and molecular imaging, but projects in other fields of research are explicitly encouraged.

• Resource
• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Core provides complete CRISPR services, design, production of gRNAs & Cas9 mRNA, and injection.

• Resource
• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Core provides instrumentation in area of solution phase, high resolution NMR spectroscopy and expertise. Primary solution state spectroscopy of bio molecules including, but not limited to, studies of polymers, minerals, soils, and extracts. Equipment includes: 800MHz Varian Inova Spectrometer, 600MHz Varian Inova Spectrometer, 500MHz Varian Inova Spectrometer, and staff assistance/consultation is available.

• Resource
• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Core provides flow cytometry services to scientists, offers analytical cytometry and high-speed sorting either in investigator operated format or facility staff assisted format, offers training for research personnel interested in learning how to design flow cytometry experiments, operate instrumentation and analyze data. Services include experimental design, protocol development, sample staining, instrument operation, data analysis, graphical production, sample processing as a Service (SpaaS). Facility is equipped with three cell sorters and four cell analyzers.

• Resource
• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Core provides mass spectrometric services, from basic mass measurement to complex proteome analyses.Services include basic mass measurement, ESI-MS/MS,LC-MS,Exact mass measurement,Protein identification.

• Resource
• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Core using ultrahigh performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) offers high-throughput analytical services for determining diagnostic amino acids and their precursors or metabolic products. Available biomarkers include citruilline and tetrahydrobiopterin. Determination of other small molecules is possible.

• Resource
• SciCrunch
• 7 years ago - submitted by Edyta Vieth