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Facility specializes in high resolution chemical imaging by three dimensional atom probe tomography. APT produces three-dimensional (3D) atom-by-atom elementally and isotopically resolved image with sub-nanometer spatial resolution of sample volume typically 100 x 100 x 300 nm^3, by simultaneous high resolution direct-space imaging and atom-by-atom time of flight mass spectrometry. APT is particularly suited to study nano- or nanostructured materials.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Core provides technical expertise, training, instrumentation and related services for assessing behavior in animals to help to gain insight into function of nervous system and mechanisms of acute and chronic pain. Services include Behavioral Testing; General Behavioral Phenotyping: Observational Screens, Motor Function; Pain/Sensory Testing: Thermal, Mechanical, Chemical; Additional Neurobehavioral Tests: Psychiatric, Addiction, Learning and Memory;Miscellaneous Systems: GI Transit, Cardiovascular and Respiration. Other Services: Assistance choosing behavioral test and statistical tests for assessing results; Training in surgical techniques for small animal surgeries; Staff expertise includes surgical methods for producing pain models; dosing of drugs/anesthetics including central, systemic and localized administration; and tissue extractions.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Core provides viral vector services dedicated to development and production of viral vectors for angiogenesis, stem cell and regenerative medicine and clinical renal research. Viral vectors are produced in dedicated BL2 facility within MMCRI building. Vector systems currently being used include adenovirus, retrovirus and lentivirus. Services Available:Transfection and transduction of gene-specific viral DNA provided by principal investigators;Small, large and custom scale production of adenovirus;retrovirus and lentivirus (2nd and 3rd generation packaging);TCID50 titration of adenovirus;Titration of retrovirus and lentivirus (fluorescence marker or antibiotic resistance);Test for replication competent virus (RCV) in lentivirus preparations.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Core offers researchers, clinicians and biotech companies regulatory compliant labs and services to manufacture minimally manipulated and complex biotechnology products.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Core offers multiple types of experiments from simple protein identification to protein quantitation (both relative and absolute), performs both traditional bottom-up proteomics, where proteins are digested with enzyme prior to analysis and intact, top-down proteomics analyses. Core proteomics specialists help guide your project to completion.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Core provides expertise and resources for large scale biology. Helps to set up, run, gather data and perform analysis in drug discovery research, biochemistry, cell and organismal biology, functional genomic screening, and synthetic genetic. Works with proteins, nucleic acids, small model organisms, and microbial strains. Provides tissue culture,produces and uses lentivirus particles, screens compound libraries, does experiments for investigators,generates preliminary data to figure out if idea is workable, discusses project development. Services include Macromolecular binding, biochemical, and cell-based assays,High content screening with widefield or confocal optics,Nanoliter liquid handling up to 1536-well density,Whole-plate kinetic assays (ion currents, GPCR signaling),Compound library screening,CRISPR/Cas9 screening (multiplexed libraries),Analysis of large data sets,Fluorescence Thermal Shift assay (measures protein melting),Complex liquid handling work flows.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Core is Northwestern Radiology research facility providing translational imaging capabilities that promote pre-clinical and clinical research efforts. CTI occupies space in basement of Olson building housing imaging equipment along with research staff. Services include Cardiovascular Imaging for development, analysis and application of MRI methods providing insights into structure and function of cardiovascular system,NeuroImaging for functional MRI using spectroscopy and diffusion-weighted imaging to studying human anatomy and physiology during development and disease,Small Animal Imaging for molecular and functional imaging of biological processes in living animal models to study diseases and responses to intervention.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Core provides mechanical testing machines and accessories for conducting educational, research, and outreach experiments on most solid materials.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Core provides high-resolution instruments capable of quantitatively imaging biologically essential elements in individual cells. Used for analyzing metal quotas at scales ranging from subcellular level to entire ecosystems.Provides access to imaging and quantification instrumentation while supporting its use with an expert technical staff that offers range of services, including instrument training, sample preparation and analysis, experiment design, and grant proposal assistance.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Shared use and training facility. Servies include Confocal Laser Scanning Microscopy, DIC (Differential Interference Contrast Microscopy), FCS (Fluorescence Correlation Spectroscopy), FLIP (Fluorescence-Loss-In-Photobleaching), FRAP (Fluorescence Recovery After Photobleaching),FRET (Fluorescence/ Forster Resonance Energy Transfer),Live-cell Imaging,Phase Contrast Microscopy,Widefield Fluorescence Microscopy,Image Processing and Analysis. Services include poster printing, and specimen preparation. Provides training for most instruments. Instruments include Leica DM6B Fluorescent Microscope (Hogan 5-112), Leica TCS SP8 Confocal Microscope (Hogan 5-128),Leica SP5 II Confocal Microscope (Hogan 5-114),Leica Spinning Disk Confocal Microscope (Hogan 5-113),DeltaVision Deconvolution Microscope (Hogan 5-111),Olympus IX83 Inverted Fluorescent Microscope (Silverman Hall 1-567),Olympus IX53 Inverted Color Microscope (Silverman Hall 1-567), LionHeart Automated Microscope BioTeck (Hogan 5-110).

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Core provides access to NMR, mass spectrometry, X-Ray crystallography instruments and education on use of instrumentation needed for molecular structure characterization.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Core provides iPSC technology services.Specialize in creating custom patient-derived iPSCs, providing training in stem cell culture, project consultation, CRISPR gene editing services, cell banking, and facility usage.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Core provides quality controlled recombinant proteins. rPPC operates based on the two service models: Training model where Northwestern researchers use specialized bioreactor systems and participate in hands-on-training activities and Production model where staff carry out expression (mg to gm scale) and purification of recombinant or synthetic biologics, including potential therapeutic proteins and peptides, among others. Main focus of rPPC is to be user-facility;facility has parallel bioreactor systems for multiplexed lab-scale cultivation of microbial, insect, and mammalian cells. rPPC also serves as a production facility, providing low-cost recombinant biologics for researchers at Northwestern University.Services include: TRANSFECTION/TRANSFORMATION, ANALYTICAL (SMALL SCALE) EXPRESSION IN E.COLI AND MAMMALIAN CELLS,PROTEIN EXPRESSION IN E. COLI (LARGE SCALE),PROTEIN EXPRESSION IN MAMMALIAN CELL SYSTEM (LARGE SCALE),PROTEIN EXPRESSION IN INSECT CELL SYSTEM,GENERATION OF MOUSE HYBRIDOMA, PRODUCING MONOCLONAL ANTIBODIES, per one fusion,RESCUING AND CULTIVATING EUKARYOTIC CELLS,DOWNSTREAM PROCESSING OF GROWN CULTURE (BEFORE PROTEIN PURIFICATION),RECOMBINANT PROTEIN PURIFICATION,TAG CLEAVAGE WITH TEV. PROTEASE,LARGE SCALE mAb PRODUCTION ,DNA PLASMID PROPAGATION AND PURIFICATION,SDS-PAGE ANALYSIS,WESTERN BLOT ANALYSIS, INSTRUMENT TIME SHARING TECHNICAL/INSTRUMENT ASSISTANCE TIME,PROTEIN RECOVERING FROM INCLUSION BODIES.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Core provides hormone analyses for both human and animal studies for scientific community at Northwestern, small rodent metabolic phenotyping resources for diabetes, obesity and other metabolic research.Maintains quality control and standardize internal references for hormone assays.Maintains reference database information on mouse metabolic phenotyping models from Northwestern University community.Services include:Immunoassays for endocrine hormones, assays using technology from Luminex,Lipid and glucose chemical analyeses,Roche Cobas e 411 analyzer employing sophisticated electrochemiluminescence (ECL) technology to deliver excellent low-end sensitivity and broad dynamic range of assays including metabolic and reproductive hormones, thyroid function test, cardiac markers, maternal care, tumor markers and bone turnover markers.,TSE LabMaster Automated Phenotyping system is a state-of-the-art system for studying energy metabolism at whole animal level.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Core provides assistance with standard and customized research specific histology services for variety of tissues (i.e., mouse, rat, sheep, pig, zebrafish, etc.) for routine histology, and immunohistochemistry. Provides Pathologist consultation to help develop strategies to elucidate phenotypes and gain mechanistic insight regarding biologic actions of targeted molecule(s) or toxicity of exogenously administered substances.Provides training opportunities for learning histology techniques and phenotyping analysis.Services include Immunohistochemistry (IHC) . Using tyramide signal amplification (TSA) and ABC methods (automated), with DAB chromogenic substrate; Immunofluorescence (IF)- Single and multiple staining;TUNEL assay; Freezing and embedding of tissues for histology;Frozen sections;Paraffin block processing and Paraffin sections;Hematoxylin and Eosin staining of sections;Specialized histochemical staining of sections (e.g. Trichrome, PAS, Luxol fast blue, Cresyl violet, and many other stains);Dissection and tissue collection;Histopathology (slide interpretation);Histopathology toxicity evaluation;Training in necropsy techniques including perfusion of mice and special tissue dissection;Assistance with development of animal pathology protocols.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Facility provides liquid helium and liquid nitrogen to researchers and laboratories at Northwestern University.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Core provides multiple technique characterization of variety of surfaces with regard to atomic structure, surface chemical composition, and chemical bonding characteristics. Equipment includes:AFM/Nanoindentor for high resolution imaging and nanoindentation studies;Laser writing for generating controlled surface roughness and patterns;Optical profilometers: Two optical interferometers (Zygo and Aliconia) are available for surface topographic imaging;Three-target magnetron sputter-deposition system. Provides Training Seminars/Workshops.Provisions have been made to accommodate routine short term measurements by user groups as well as more extended studies. User and research groups are encouraged to work with facility staff to tailor facility resources to research project.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Provides immunochemical, chemistry and immune testing to clinical and basic science researchers, develops and validates new assays, and improves current assay methodology. Services include Assay development, Automated chemistry analysis, Immunoassay analysis, Small molecule analysis,Mass Spectrometry, LC-MS/MS and HRAM LC-MS,Enzyme-linked immunosorbent (ELISA), Meso Scale Discovery (MSD), Luminex, and radioimmunoassay (RIA) procedures for various analytes.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Core provides general purpose x-ray equipment for diffraction and fluorescence studies. Examples of current measurements are: powder diffraction, single-crystal diffraction, thin-film reflectivity, thin-film diffraction, crystal truncation rod scattering, small angle scattering, Laue diffraction, residual stress, pole figures, EDX-ray fluorescence, x-ray standing waves, high-resolution x-ray diffraction, and grazing incidence small angle scattering.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Core provides Next Generation Sequencing and Bioinformatics services.Services include:RNA assessment using Agilent 2100 Bioanalyzer,Next Generation Sequencing:Single cell RNA-seq,DNA-seq,RNA-seq,miRNA-seq,ChIP-seq,Microbiome (16S rRNA),ATAC-seq,Data Analysis (Partek Flow, Transcription Analysis Console).

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth