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One of the eleven Ministries of Japan that composes part of the executive branch of the Government of Japan. Their goal is to improve the development of Japan in relation with the international community.

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• SciCrunch
• 14 years ago - submitted by Anita Bandrowski

Core provides cell analysis, sorting instruments and expertise in regular fluorescence, spectral, and flow cytometry. Offers educational and cytometric services including stem cell analysis, immunophenotyping, cell cycle analysis, translocation and co-localisation of cell activation markers, chromatin density, and apoptotic and necrotic analyses. Performs cell sorting, expertise in experimental design and provides equipment training for all users.

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• RRID
• 10 months ago - submitted by Richard Grenfell

Welcome to ZINC, a free database of commercially-available compounds for virtual screening. ZINC contains over 13 million purchasable compounds in ready-to-dock, 3D formats. ZINC is provided by the Shoichet Laboratory in the Department of Pharmaceutical Chemistry at the University of California, San Francisco (UCSF). To cite ZINC, please reference: Irwin and Shoichet, J. Chem. Inf. Model. 2005;45(1):177-82 PDF, DOI. We thank NIGMS for financial support (GM71896). There are release notes for ZINC 10. - We have a survey where you can give us feedback.

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• SciCrunch
• 17 years ago - by Anonymous

Consumer product safety commission charged with protecting the public from unreasonable risks of injury or death associated with the use of the thousands of types of consumer products under the agency''s jurisdiction - such as toys, cribs, power tools, cigarette lighters, and household chemicals. CPSC is committed to protecting consumers and families from products that pose a fire, electrical, chemical, or mechanical hazard.

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• SciCrunch
• 15 years ago - submitted by Andrea Stagg

Core enables generation of genetically defined human iPSC-derived hepatocytes, CRISPR/Cas9 mediated gene editing, and primary mouse hepatocyte isolation.

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• SciCrunch
• 9 months ago - submitted by Edyta Vieth

Biological Concept Diagram Editor (BCDE) is a conceptual relationship diagramming tool specifically designed for biomedical researchers. It allows for efficient knowledge and data capture, fast diagram creation, easy data retrieval, and flexible exporting. The BCDE application is the main diagramming tool in the system. Through it, users can create, modify, load, and save BCDE diagrams. The diagrams created with BCDE application are network oriented. Each BCDE figure can be annotated using fields from the BioPAX level II format. In addition, a user can add URL links and attachments to a BCDE figure. Diagrams generated in BCDE are stored in the BCDE XML format for better database integration and better data extraction.

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• SciCrunch
• 17 years ago - by Anonymous

Federal agency.

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• SciCrunch
• 14 years ago - submitted by Andrea Stagg

Core generates transgenic and gene-targeted mice for researchers at Mayo Clinic's campuses in Arizona, Florida and Minnesota to enable researchers to observe how genes, or mutant variations of genes, are expressed. Provides services to all Mayo Clinic faculty and staff engaged in research. Also, services are offered to non-Mayo investigators and research collaborators.

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• SciCrunch
• 9 months ago - submitted by Edyta Vieth

Software tool for data sharing, incorporating blogs and spatial registration of data. Mainly used in geological data sets. A Virtual Research Environment (VRE) aims to combine the capabilities of two existing technologies that have already seen wide adoption among scientists: - The Godiva2 data visualization system provides a means for scientists to browse interactively in a ''Google Maps-like'' fashion through large environmental datasets, including numerical model outputs and high-resolution satellite imagery, using only a web browser. - The LabBlog is a web-based blogging tool specifically designed for the practising scientist to record, disseminate and evaluate their research. The Blog can also be used as a collaboration tool that allows secure discussion between colleagues. Although initially designed for the use of laboratory chemists, the LabBlog is being adapted in this project to meet the needs of environmental scientists. The BlogMyData VRE will allow scientists to explore data visually using Godiva2, then make comments about features in the data on a blog. Colleagues will discover these blog entries and offer further information, providing answers to research questions through comments. Through RSS and GeoRSS feeds, colleagues, investigators and other interested parties can be notified of research activity, and scientists can discover hitherto-unknown colleagues working with similar data in similar geographic regions. Sponsors: BlogMyData is a collaboration between the Reading e-Science Centre and the University of Southampton and is one of the JISC VRERI projects.

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• SciCrunch
• 17 years ago - by Anonymous

Government granting agency that works 24/7 to protect America from health, safety and security threats, both foreign and in the U.S. CDC Office of Financial Resources aids in achieving CDC’s mission by quickly and effectively allocating funds to where they are needed.

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• SciCrunch
• 15 years ago - submitted by Timothy Tsui

Facility supporting analytical needs for materials. Provides advanced facilities and equipment for materials characterization, surface, optical and structural analysis, elemental composition, and high-resolution electron microscopy. The center supports materials analysis across a broad range of scientific disciplines, including physics, chemistry, life sciences, earth and space sciences, environmental sciences, and engineering. Offers electron microscopy, X-ray diffraction, ICP-MS, spectroscopy, and related techniques.

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• RRID
• 9 months ago - by Anonymous

The data sets included in this resource are published in Kravitz et al, 2012 Distinct roles for direct and indirect pathway striatal neurons in reinforcement (Nat. Neurosci). The data shows: optogenetic activation of dopamine D1 or D2 receptor-expressing striatal projection neurons influenced reinforcement learning in mice. Stimulating D1 receptor-expressing neurons induced persistent reinforcement, whereas stimulating D2 receptor-expressing neurons induced transient punishment. These 3 recording files contain data collected in 2010 and 2011 by Lex Kravitz in Anatol Kreitzer''s lab at the Gladstone Institutes. Each file contains ~ 1 hour of awake in vivo recording data, containing the spike times for ~30 minutes of spontaneous activity, preceeded or followed by 400 laser pulses (473nm laser light, 1 sec pulses, 3 seconds inter-pulse-interval). The laser pulses were presented at 4 intensities: 0.1mW, 0.3mW, 1.0mW, and 3.0mW, and pulse times at each intensity are given in separate columns in each data file. Finally, each data file contains the identification of ''light-modulated units'' as we identified them (see methods below). Methods: Viral expression of DIO-ChR2-YFP and DIO-YFP We used double-floxed inverted (DIO) constructs to express ChR2-YFP fusions and YFP alone in Cre-expressing neurons, which virtually eliminates recombination in cells that do not express Cre-recombinase (Sohal et al, Nature, 2009). The double-floxed reverse ChR2-YFP or YFP cassette was cloned into a modified version of the pAAV2-MCS vector (Stratagene, La Jolla, CA) carrying the EF-1a promoter and the Woodchuck hepatitis virus posttranscriptional regulatory element (WPRE) to enhance expression. The recombinant AAV vectors were serotyped with AAV1 coat proteins and packaged by the viral vector core at the University of North Carolina. The final viral concentration was 4 x 10^12 virus molecules/mL (by Dot Blot, UNC vector core). This viral construct can now be ordered in single aliquots directly from UNC Vector core as product AAV-EF1a-DIO-hChR2(H134R)-EYFP, at http://genetherapy.unc.edu/services.htm) Implantation of electrode arrays for awake recordings Anaesthesia was induced with a mixture of ketamine and xylazine (100mg ketamine plus 5mg xylazine per kilogram of body weight i.p.) and maintained with isoflurane through a nose cone mounted on a stereotaxic apparatus (Kopf Instruments). The scalp was opened and a hole was drilled in the skull (0.0 to +1.0mm AP, -1.0 to -2.0mm ML from bregma). Two skull screws were implanted in the opposing hemisphere. Dental adhesive (C&B Metabond, Parkell) was used to fix the skull screws in place and coat the surface of the skull. An array of 16 or 32 microwires (35-��m tungsten wires, 100-��m spacing between wires, 200-��m spacing between rows; Innovative Physiology) and one optical fiber in a ferrule was lowered into the striatum (3.0mm below the surface of the brain) and cemented in place with dental acrylic (Ortho-Jet, Lang Dental). After the cement dried, the scalp was sutured shut. Animals were allowed to recover for at least seven days before striatal recordings were made. In vivo electrophysiology Voltage signals from each recording site on the microwire array were band-pass-filtered, such that activity between 150 and 8,000Hz was analysed as spiking activity. This data was amplified, processed and digitally captured using commercial hardware and software (Plexon). Single units were discriminated with principal component analysis (OFFLINE SORTER, Plexon). Two criteria were used to ensure quality of recorded units: (1) recorded units smaller than 100��V (~3 times the noise band) were excluded from further analysis and (2) recorded units in which more than 1% of interspike intervals were shorter than 2ms were excluded from further analysis. Average waveforms were exported with OFFLINE SORTER. During the recording we coupled the array to a laser and pulsed the laser at four intensities (0.1mW, 0.3mW, 1mW, and 3mW). Laser stimulation was run in a cyclical fashion, on for 1 second, and off for 3 seconds. Each neuron received 100 pulses at each laser intensity. Identification of ChR2 expressing units in in vivo recordings For all neurons, peri-event histograms were generated for each laser intensity independently. Neurons were classified as ChR2-expressing if they exhibited a firing rate greater than 3x above the standard deviation of the 1-second preceding the laser pulse within 10msec of the laser onset. Each neuron was tested independently at each laser power, and neurons that satisfied this criteria at any one power were defined as ChR2-expression.

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• SciCrunch
• 16 years ago - by Anonymous

United States federally funded research and development center supported by the National Cancer Institute and managed by the private contractor Leidos Biomedical Research.

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• SciCrunch
• 16 years ago - submitted by Andrea Stagg

Web application to plan and document cloning and genome engineering. Open source and open access tool, FAIR data principles compatible, designed to simulate and/or document genetic manipulation processes used in every day synthetic biology and molecular biology.

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• RRID
• 8 months ago - submitted by Luisa Fernanda Jimenez Soto

Laser tool that enables neurons to be optically silenced by pulses of yellow light, the light-activated chloride pump halorhodopsin (Halo), in a paper entitled Multiple-color optical activation, silencing, and desynchronization of neural activity, with single-spike temporal resolution. Temporally precise, noninvasive control of activity in well-defined neuronal populations is a long-sought goal of systems neuroscience. We adapted for this purpose the naturally occurring algal protein Channelrhodopsin-2, a rapidly gated light-sensitive cation channel, by using lentiviral gene delivery in combination with high-speed optical switching to photostimulate mammalian neurons. We demonstrate reliable, millisecond-timescale control of neuronal spiking, as well as control of excitatory and inhibitory synaptic transmission. This technology allows the use of light to alter neural processing at the level of single spikes and synaptic events, yielding a widely applicable tool for neuroscientists and biomedical engineers. The quest to determine how precise neural activity patterns mediate computation, behavior, and pathology would be greatly aided by a set of tools for reliably activating and inactivating genetically targeted neurons, in a temporally precise and rapidly reversible fashion. Having earlier adapted a light-activated cation channel, 1channelrhodopsin-2 (ChR2), for allowing neurons to be stimulated by blue light, we searched for a complementary tool that would enable optical neuronal inhibition, driven by light of a second color. Here we report that targeting the 1codon-optimized form of the light-driven chloride pump halorhodopsin from the archaebacterium Natronomas pharaonis (hereafter abbreviated Halo) to genetically-specified neurons enables them to be silenced reliably, and reversibly, by millisecond-timescale pulses of yellow light. We show that trains of yellow and blue light pulses can drive high-fidelity sequences of hyperpolarizations and depolarizations in neurons simultaneously expressing yellow light-driven Halo and blue light-driven ChR2, allowing for the first time manipulations of neural synchrony without perturbation of other parameters such as spiking rates. The Halo/ChR2 system thus constitutes a powerful toolbox for multichannel photoinhibition and photostimulation of virally or transgenically targeted neural circuits without need for exogenous chemicals, enabling systematic analysis and engineering of the brain, and quantitative bioengineering of excitable cells.

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• SciCrunch
• 15 years ago - by Anonymous

The National Natural Science Foundation of China (NSFC) is an organization directly affiliated to the State Council for the management of the National Natural Science Fund. # In accordance with the guiding principles, policies and plans for the development of science and technology in China and by adopting the operating mechanisms for the National Natural Science Fund which conform to the socialism market economic system, NSFC supports basic research and some of applied research, identifies and fosters talented researchers in the realm of science and technology, accelerates the progress of science and technology, and promotes the socioeconomic development in China by giving full play the guidance and coordinating role of the National Natural Science Fund from the central government. # NSFC is in charge of the management of the National Natural Science Fund. It compiles and promulgates the annual Guide to Programs for basic research and some of applied research, handles applications of research projects, and organizes peer review and select the best projects to support. It endeavors to create an academic environment conducive to innovation. # NSFC cooperates with the Ministry of Science and Technology to formulate the principles, policies and plans for the development of basic research in China. When entrusted, it provides consultation and undertakes related assignments on major issues for the development of high technology and applied research in China. # NSFC gives help and support to other Chinese foundations in natural sciences. # NSFC establishes contacts with governmental departments in charge of science and technology, science foundations and related academic organizations in other countries and carries out international cooperation and exchange. # NSFC is responsible for the administration, supervision and guidance of the subordinated bodies. # NSFC undertakes other tasks entrusted by the State Council and the State Leading Group for Science and Technology and Education.

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• SciCrunch
• 13 years ago - submitted by Andrea Stagg

Software R package which implements simple single-sample gene signature scoring method that uses rank-based statistics to analyze sample's gene expression profile. Scores expression activities of gene sets at single-sample level. Single sample scoring of molecular phenotypes.

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• RRID
• 8 months ago - by Anonymous

An R extension to ANTs that performs multivariate statistical parametric mapping of DTI, T1 and other datatypes for the purpose of both performing clinical studies and for tracking the performance of ANTs (and other) image processing methodologies. ANTsR depends upon the R statistical language, bash scripts and the ANTs toolkit. Some branches of ANTsR will also depend upon pipedream and specific datasets. Some of these datasets will be open access and, in that case, ANTsR will provide a 100% reproducible neuroimaging study on that data.

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• SciCrunch
• 15 years ago - by Anonymous

A company of professionally trained stereologists, bioscientists, computer engineers, educators, and support personnel that provides comprehensive neurostereology products and services to the global bioscience community. These products and services include: stereology and image analysis software, digital imaging technology, turnkey stereologer solutions, comprehensive stereology and image analysis/image processing software, professional contract services, neurostereology workshops, basic stereology courses, and online stereology webinars. The Center also offers tissue processing (sectioning, staining, slide preparation) and stereology data collection services. Researchers may use equipment and facilities to complete projects with full confidentiality and according to Good Laboratory Practices guidelines.

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• SciCrunch
• 13 years ago - by Anonymous

Facility provides access to nuclear magnetic resonance spectroscopy for all. Houses spectrometers ranging in field strength from 400 to 600 MHz, including two 500 MHz Brukers equipped with nitrogen-cooled Prodigy CryoProbes. New users may operate a particular spectrometer independently once they complete the required training and demonstrate the knowledge required for operating each instrument.

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• SciCrunch
• 8 months ago - submitted by Edyta Vieth