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Pipette puller that fabricates pipettes for use in intracellular recording, patch clamping, transferring ICSI, ES Cells , microinjection, aspiration, and microperfusion. P-97 puller can indirectly sense viscosity of glass, giving the ability to pull pipettes from all glasses except quartz. Even difficult to pull formulations, such as aluminosilicate glasses, are handled with relative ease.

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• SciCrunch
• 6 years ago - submitted by Edyta Vieth

Quickly seal in heat with the Thermo Scientific ALPS 3000 Automated Microplate Heat Sealer. This compact sealer is designed for optimal robotic integration in high throughput labs, yet still allows for manual benchtop control.

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• SciCrunch
• 6 years ago - submitted by Edyta Vieth

COBE 2991 cell processor offers an alternative to labor-intensive methods for the separation of cell products, such as manually layering blood over a density gradient separation medium using conical tubes. These methods involve a large number of operator steps and manual pipetting techniques that require significant practice, introducing variability and the opportunity for operator error. Automated processing puts control at the user's fingertips. Protocols can be easily programmed for efficient, reproducible results while saving time and reducing the number of manual operating steps. For maximum flexibility, nearly all aspects of operation can be controlled manually. operation saves training time, requires little technical support and reduces the opportunity for operator errors.

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• SciCrunch
• 6 years ago - submitted by Edyta Vieth

Microprocessor controlled, CO2 laser based micropipette puller.Default configuration allows fabrication of micropipettes for intracellular recording, patch clamping, microinjection and microperfusion.Can store up to 100 separate programs, each consisting of up to 8 command lines. The five parameters set in each command line allow exquisite control of the micropipette taper geometry. Allows the use of wide range of glass diameters from 0.125 to 1.2 mm.

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• SciCrunch
• 6 years ago - submitted by Edyta Vieth

Luminescent assays are sensitive and quantitative tools commonly used for a variety of purposes in biomedical and pharmaceutical research. Quantitation of ATP can be used to detect and measure cellular growth, as well as bacterial contamination. Luminescent genetic reporting assays are widely used to study gene expression and cellular responses to external stimuli in Prokaryotic and Eukaryotic organisms. Dual-reporter assays use two independent reporter systems simultaneously to improve experimental accuracy. One reporter is used to measure the response resulting from the experimental conditions and is often referred to as the “experimental� reporter. The second reporter is designed not to respond to the experimental conditions, acting as an internal control from which data generated by the experimental reporter can be normalized. Normalization of the data serves to compensate for variability caused by differences in transfection efficiency, cell viability, cell lysis, and pipetting. Promega's Dual-Luciferase System uses the activities of luminescent proteins (luciferases) from the firefly (Photinus pyralis) beetle and the sea pansy (Renilla renformis) to serve as an experimental and a control reporter respectively (See Figure 1). The Synergy HT Multi-Detection Reader (BioTek Instruments, Winooski, VT) is a robotic compatible microplate reader that can measure absorbance, fluorescence, and luminescence in all plate formats up to 384-well plates with performance in all three detection modes, that has recently been optimized for luminescence measurements (Figure 2). The Synergy HT utilizes a dual optics design that has both a monochromator/xenon flash system with a silicone diode detector for absorbance and a tungsten halogen lamp with blocking interference filters and a photomultiplier tube (PMT) detector for fluorescence. Reagent addition is accomplished by an optional external injector module, which controls two independent injector syringes. Each syringe is connected to a separate injector tip. Emitted luminescence is captured using either the top or bottom probes and measured using the Synergy HT's low noise PMT operated in photon integration mode. Here we describe the use of a Synergy HT Multi-Detection Microplate Reader (BioTek Instruments) to perform dual-luciferase measurements with purified recombinant enzymes.

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• SciCrunch
• 6 years ago - submitted by Edyta Vieth

Computer controlled manual wafer load profilometer with Max wafer size of 8"/200mm that measures roughness, waviness, step height, and other surface characteristics. Capabilities of this instrument include: Measurement of vertical features ranging from under 100Ã… (0.4 min.) to approximately 300 �m (11 mils), with a vertical resolution of 0.5, 2, or 10Ã…, A virtually unlimited number of data points per profile guarantee that the horizontal resolution is limited by the stylus radius and not by the number of data points, Measurement of many roughness and waviness parameters, with user-selectable cutoff filters to isolate roughness and waviness, Ability to fit and level a scan, allowing accurate step height measurements on curved surfaces, and the Ability to detect the edge or apex of a profile feature, allowing automated data analysis relative to the feature. The KLA P-10 to repeat a scan up to ten times and automatically calculate the average, thereby minimizing the effects of environmental noise on measurements. KLA-Tencor P10 can profile a variety of materials including: Semiconductor wafers, Thin-film heads, Precision-machined and polished surfaces, Ceramics for micro-electronics, Glass for flat panel displays, and Optical surfaces

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• SciCrunch
• 6 years ago - submitted by Edyta Vieth

Powered by either 1 x 1.34 GHz or 2 x 1.5 GHz UltraSPARC IIIi processors, the Sun Fire V240 server is an entry-level, data center-class server. It provides high availability and expandability in a compact, 2U package. Four autonegotiating Gigabit Ethernet ports provide multiple connectivity for high-speed, high-bandwidth networking, while hot-swappable, front-accessible disk drives and redundant, hot-swappable power supplies (with independent cords) further enhance the Sun Fire V240 server's uptime. With expandability features such as three PCI slots, up to four disk drives, and 16 GB of memory, the rack-optmized Sun Fire V240 server easily scales to meet the needs of Tier 1 and Tier 2 applications. With a low-cost, integrated SSL option on the motherboard, the Sun Fire V240 server provides fast, secure Web transactions without sacrificing CPU cycles or a PCI slot. The standard System Configuration Card and serviceability features help enable quick upgrades and repairs, while the unit's Sun Advanced Lights Out Manager (ALOM) provides remote management to a large number of servers from virtually anywhere on the network.

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• SciCrunch
• 6 years ago - submitted by Edyta Vieth

MA/BA6 mask aligner is designed for all standard lithography applications and wafer sizes of up to 150 mm. For thick-resist MEMS applications, the MA/BA6 offers high resolution and optimum edge quality. The bottom side alignment option allows for pattern printing on both sides of the substrate. The MA/BA6 additionally provides tailored features for fragile III-V compounds, thinned or warped wafers, transparent substrates and pieces or single dies.

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• SciCrunch
• 6 years ago - submitted by Edyta Vieth

UV crosslinker designed to crosslink DNA or RNA to nylon, nitrocellulose, or nylon reinforced nitrocellulose membranes in 25 to 50 seconds. It is equipped with internal photodetector to compensate for aging ultraviolet bulbs.

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• SciCrunch
• 6 years ago - submitted by Edyta Vieth

nCS1 particle counter is a tool for obtaining detailed size and concentration information about particle distributions in liquids.

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• SciCrunch
• 6 years ago - submitted by Edyta Vieth

Siemens 3T MAGNETOM Skyra scanner with new software that enables rapid imaging.

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• SciCrunch
• 6 years ago - submitted by Edyta Vieth

LectroPol-5 is designed for automated electrolytic polishing and etching of metallographic specimens. A scanning function for easy determination of parameters, built-in safety features, and a database with methods for various materials enables short polishing times and maximum reproducibility. LectroPol-5 consists of two units, the control unit and the polishing unit. The two units can be separated from each other. LectroPol-5 features a scanning function that helps to determine the correct voltage for both polishing and etching, thus saving time and avoiding human error. LectroPol-5 displays the scan curve, while the user selects and sets the voltage. The LectroPol-5 comes with ten polishing/etching methods for various materials, enabling immediate preparation of a wide range of materials without any lengthy and time-consuming trials.

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• SciCrunch
• 6 years ago - submitted by Edyta Vieth

SY3200 cell sorter platform is designed for configurability to adapt to the demanding needs of a variety of sorting labs. to any system in flow cytometry, the SY3200 is expandable to a dual system layout, effectively enabling two systems in one.

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• SciCrunch
• 6 years ago - submitted by Edyta Vieth

SpectraMax M2 and M2e Systems from Molecular Devices are multidetection microplate readers with dualmonochromators, dual-mode cuvette ports, and top- and bottom-reading capability (top-reading only on the M2). Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI).

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• SciCrunch
• 6 years ago - submitted by Edyta Vieth

Agilent Mx3000P QPCR System has been replaced with the AriaMx system. Used for gene expression analysis, microarray data validation, SNP genotyping, pathogen detection, DNA methylation analysis, and chromatin immuno-precipitation studies. Agilent qPCR software, MxPro, provides users with an interface, quick experiment design, data analysis and easy report generation.

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• SciCrunch
• 6 years ago - submitted by Edyta Vieth

Multifunction phosphoimager/fluorimager laser scanner used to collect fluorescent or storage phosphor images from gels, membranes and phosphor screens.

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• SciCrunch
• 6 years ago - submitted by Edyta Vieth

SORVALL MT-6000 improves the accuracy and reproducibility of the time-tested SORVALL mechanical advance system. Other improvements include a new fiber optics approach lighting system, a diffuse lighting and viewing system, a highly effective built-in vibration dampening system, precise digital speed and thickness controls, and the Visutrac system. All combine to make the MT-6000 an excellent microtome for electron or light microscopy.

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• SciCrunch
• 6 years ago - submitted by Edyta Vieth

Thermo Scientific Sorvall RC 6 Plus Centrifuge has been discontinued. Please consider our recommended replacement: Thermo Scientific Sorvall LYNX Superspeed Centrifuge Series.

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• SciCrunch
• 6 years ago - submitted by Edyta Vieth

SY3200 cell sorter platform is expandable to a dual system layout, effectively enabling two systems in one.

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• SciCrunch
• 6 years ago - submitted by Edyta Vieth

Automated cell sorter that has microfluidics sorting chip integrated with six fluorescence detectors and up to four collinear excitation lasers of 488 nm, 405 nm, 561 nm and 638 nm.

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• SciCrunch
• 6 years ago - submitted by Edyta Vieth