Curator Dashboard

Comprises six service groups. Our services are available to all NIH intramural researchers, and by interagency agreements to FDA and US Army at Ft. Detrick. We are happy to consult and distribute materials to not-for-profit institutions.Clone Optimization service include DNA cloning for protein expression; Gateway-based; fluorescent, epitope, solubility, purification tags; mutagenesis; vectors for insect/ baculovirus, mammalian, E. coli, P. pastoris, K. lactis. Microbial Expression: E. coli, P. pastoris, K. lactis, 2 mL to 60 L, autoinduction and IPTG; fully instrumented fermenters.Eukaryotic Expression service include insect / baculovirus, mammalian transient and stable, monoclonal antibodies.Protein Purification service include parallel microscale for process development; affinity and native, tag and endotoxin removal; scale-up to low gram scale.Virus Technology service include custom and stock adenovirus and lentivirus production; titer determination; promoter and reporter panels in lentivirus ready to deliver.Molecular Detection: qPCR and ELISA assaysdeveloped and executed, focused on human viruses in human samples; qPCR assays for mycoplasma and human pathogenic viruses contaminating cell lines; cell line identity testing.

• Resource
• SciCrunch
• 5 years ago - submitted by Edyta Vieth

Core provides services related to manipulation and delivery of nucleic acids to biological systems. Services include Production and titering of recombinant viral vectors,including Lentiviruses, Adenoviruses, and Adeno associated viruses;Subcloning;Tagging of open reading frames;Development of plasmids and/or RNAs for CRISPR/Cas manipulation;piggyBac transposon delivery systems;Real-time PCR analysis;Recombineering;In vitro transcription; Core maintains stock of plasmids that provide components for building constructs envisioned by investigator and provides consultative services.Provides training opportunities in the technologies described above, either as a for-credit, one semester class or on a project-related, fee-based basis.

• Resource
• SciCrunch
• 5 years ago - submitted by Edyta Vieth

Supports generation and utilization of genetically modified mice, design and production of genetic constructs with methods including yeast and E.coli recombineering, ES cell targeting (transfection, drug selection, and expansion). Produces transgenic mice: DNA injections into oocytes, ES cell injections into blastocysts.Provides humanized immune system mouse models.Offers start to finish experiment services to assist your many needs, please ask for more info.Other services include animal husbandry, IP and IV injections, blood drawing, necropsy and surgeries- embryo transfers, ovariectomy, vasectomy, tumor removal.

• Resource
• SciCrunch
• 5 years ago - submitted by Edyta Vieth

Provides instrumentation, supplies, and expertise to assist researchers in structural characterization of biological macromolecules. Resources are available for crystallization and structure determination as wells as biophysical characterization of macromolecular stability and interactions.

• Resource
• SciCrunch
• 5 years ago - submitted by Edyta Vieth

Web service to generate DsiRNAs for any sequence from any species by Integrated DNA Technologies.

• Resource
• RRID-Legacy
• 5 years ago - by Anonymous

Literature mining web service that searches all PubMed abstracts for user provided gene symbols in conjunction with set of custom keywords organized into customized ontology, as well as results from human genome wide association studies (GWAS). Searches gene symbols against list of keywords in PubMed to extract sentences that contain at least one gene and one keyword. Used to mine PubMed for gene relationships using custom ontology and deep learning.

• Resource
• RRID-Legacy
• 5 years ago - by Anonymous

Device for automating tail immersion assay. Used for measuring acute thermal pain in rodents. Software recognizes several specific RFID keys as user interface commands, which allows TailTimer to be operated via RFID fobs.

• Resource
• RRID-Legacy
• 5 years ago - by Anonymous

Database for classifying allergenic proteins into protein families.You can browse lists of allergen families restricted by allergen source (plants, animals, fungi, bacteria) and route of exposure (inhalation, ingestion etc.) or search for specific allergens, sources or protein families. Every allergen family entry is linked to description of biochemical properties and allergological significance of family members as well as list of key references related to this family.

• Resource
• RRID-Legacy
• 5 years ago - by Anonymous

Software tool for fitting kinetic and equilibrium data based on numerical integration of rate equation from any model input user. Simulation and data-fitting software for research and education designed specifically for the field of chemical kinetics.

• Resource
• dkNET
• 5 years ago - by Anonymous

Open source software toolkit for audio feature extraction and classification of speech and music signals. Used for audio analysis, processing and classification especially targeted at speech and music applications, e.g. automatic speech recognition, speaker identification, emotion recognition, or beat tracking and chord detection.Written in C plus plus and runs on desktop, mobile, and embedded platforms such as Linux, Windows, macOS, Android, iOS and Raspberry Pi.

• Resource
• RRID-Legacy
• 5 years ago - by Anonymous

THIS RESOURCE IS NO LONGER IN SERVICE. Documented on March 23, 2022.

• Resource
• RRID-Legacy
• 5 years ago - submitted by Valentina Feodorova

Open source software tool as file archiver with high compression ratio.

• Resource
• RRID-Legacy
• 5 years ago - by Anonymous

Software tool to provide color schemes for maps and other graphics designed by CARTO.Implementation of CARTOcolor palettes in R. It includes four types of palettes: aggregation, diverging, qualitative, and quantitative.

• Resource
• RRID-Legacy
• 5 years ago - by Anonymous

GNU Project shell. Sh-compatible shell that incorporates useful features from Korn shell (ksh) and the C shell (csh). Improvements offered by Bash include command-line editing, unlimited size command history,job control,shell functions and aliases,indexed arrays of unlimited size,integer arithmetic in any base from two to sixty four.

• Resource
• RRID-Legacy
• 5 years ago - by Anonymous

Software functional programming tools for R. Used for working with functions and vectors.

• Resource
• RRID-Legacy
• 5 years ago - by Anonymous

International data on all cause mortality. Country level data on all cause mortality in 2015–2021 collected from various sources for 94 countries and territories.

• Resource
• RRID-Legacy
• 5 years ago - by Anonymous

Gait analysis system for quantitative assessment of footfalls and locomotion in rats and mice. System includes corridor that directs free movement in straight line, hardened glass walkway that enables CatWalk XT Illuminated Footprints technology,high speed color camera for spatial and temporal resolution, CatWalk XT software for recording and automated analysis of locomotor ability of rodents.

• Resource
• RRID-Legacy
• 5 years ago - submitted by Paul Mathews

System which combines 3D printed open hardware with deep segmentation networks for high temporal resolution phenotyping of plant roots in agarized medium. Deep learning based root extraction method which leverages the latest advances in convolutional neural networks for image segmentation, and incorporates temporal consistency into the root system architecture reconstruction process.

• Resource
• RRID-Legacy
• 5 years ago - submitted by Rafael Nicolás Gaggion Zulpo

Software tool as next generation microbiome bioinformatics platform that is extensible, free, open source, and community developed.Enables researchers to start analysis with raw DNA sequence data and finish with publication quality figures and statistical results. Used to analyze and interpret nucleic acid sequence data from fungal, viral, bacterial, and archaeal communities.

• Resource
• RRID-Legacy
• 5 years ago - by Anonymous

Automated tissue microarrayer, allowing precise selection and extraction of specific regions of interest on donor blocks and their insertion in recipient blocks.Controlled by TMA Control software.

• Resource
• RRID-Legacy
• 5 years ago - by Anonymous