Curator Dashboard

Facility works with investigators to obtain and analyze high-quality nutrition and body composition data from study participants. Staff and facilities are located in the Schubert Research Clinic at Cincinnati Childrens Hospital. Core provides diet assessment services.

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• SciCrunch
• 2 years ago - submitted by Edyta Vieth

Core supports data analysis, network modeling, and data science research. Provides training and education opportunities.

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• SciCrunch
• 2 years ago - submitted by Edyta Vieth

Facility includes Analytic Redox Biology and Molecular Signaling Sub-Cores. Provided services include measurements of reactive oxygen species, reactive nitrogen species, reactive sulfide species, protein, peptides, metabolites, and lipids in cell culture and tissue samples. High-performance liquid chromatography systems, coupled with UV-vis and fluorescence detectors to quantify cellular and mitochondrial superoxide production, hydrogen sulfide pools (free sulfide, sulfide bound to transitional metals, and sulfane sulfur), and thiols (glutathione, GSH/GSSG, cysteine, cystine, homocysteine, persulfides, and glutathionylation).Instruments include Sievers NO Analyzer (NOA 280i, GE), Agilent 8890 GC and 8335 sulfur chemiluminescence detector ,Thermo Scientific Orbitrap Exploris 480 and SCIEX Triple Quad 7500 mass spectrometer. Molecular Signaling Sub-Core offers services for molecular cloning and site-directed mutagenesis, design and production of vectors for CRISPR/Cas9 modification of vascular cells, services for endothelial, smooth muscle, and cardiac myocyte cell isolation, for generation of vascular cell lines, and for lentivirus production for transient or stable modification of cardiovascular cells, access to equipment and expertise for studying cellular effects of hypoxia/reoxygenation injury (Coy Hypoxic Chamber, CLARIOstar Spectrofluorometer), for automated capillary Western blot analysis (ProteinSimple Jess Simple Western), and for high-resolution fluo-respirometry to study mitochondrial function (Oroboros O2k modular system).

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• SciCrunch
• 2 years ago - submitted by Edyta Vieth

Core provides imaging support to investigators who use small animal models in their research. Offers bioluminescence and fluorescence imaging, X-ray imaging, computed tomography, and positron emission tomography. Core provides imaging support services for investigators, including assistance in experimental design, conducting animal studies including anesthetization and imaging acquisition, and processing and interpreting imaging data.

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• SciCrunch
• 2 years ago - submitted by Edyta Vieth

Core provides services for mouse genotyping and tissue histology, access to tools for analyzing cardiovascular function, data analysis, expertise and training. Services include genotyping using PCR or RT PCR, histology services with tissue processing, paraffin-embedding and sectioning, along with several cardiovascular relevant histological stains such as Picrosirius Red and Masson Trichrome.

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• SciCrunch
• 2 years ago - submitted by Edyta Vieth

Core provides access to research services, educational and training opportunities, and technologies including cellular metabolism, flow cytometry, fluorescence, confocal, super resolution confocal microscopy, next generation sequencing, and real-time PCR. Provides assistance with all aspects of these technologies, including experimental design, protocol optimization, instrument operation, and data analysis.

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• SciCrunch
• 2 years ago - submitted by Edyta Vieth

Web service for online data drawing and analysis, personalized data analysis, scientific research mapping, online page/database development and hosting. Used for high throughput sequencing data analysis including eccDNA-seq, MeRIP-seq, circRNA-seq, miRNA-seq, mRNA-seq, LncRNA-seq, piRNA-seq, ChIP-seq, etc.

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• RRID
• 2 years ago - by Anonymous

Software code package associated to the paper: Lemke et al. Information flow between motor cortex and striatum reverses during skill learning. 2023. Quantifies information theoretic quantities used in the associated study.

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• RRID
• 2 years ago - submitted by Marco Celotto

Software application as MATLAB scripts to compute measures of Feature-specific Information Transfer (FIT) and conditional FIT (cFIT). FIT quantifies direction and magnitude of information flow about specific feature S (such as feature of sensory stimulus) between simultaneously recorded brain regions X and Y. cFIT quantifies amount of directed feature information transmitted between regions X and Y that cannot be potentially routed through region Z.

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• RRID
• 2 years ago - submitted by Marco Celotto

Software finite element analysis package. Used to solve field problems.

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• RRID
• 2 years ago - by Anonymous

ACTH ELISA kit to measure ACTH levels in human, mouse and rat plasma samples. ACTH (Adrenocorticotropic hormone) or corticotropin is a 39-amino acid hormone (MW=4500) secreted by the pituitary to regulate the production of steroid hormones by the adrenal cortex. ACTH secretion from the anterior pituitary is controlled by both a classical negative feedback control mechanism and CNS-stress mediated control system. Various types of stress or pain perceived in higher levels of the brain modulate secretion of the hypothalamic neurosecretory hormone, corticotropin releasing hormone (CRH), a 41-amino acid peptide. CRH stimulates pituitary ACTH secretion. The second peptide that modulates ACTH secretion is vasopressin (AVP). AVP secretion is also stimulated by stress and acts synergistically with CRH to increase ACTH secretion in the pituitary portal circulation. ACTH increases the synthesis and release of all adrenal steroids, aldosterone, cortisol and adrenal androgens. It is the principal modulator of cortisol, the most important glucocorticoid in man. As the cortisol level in blood increases, release of ACTH is inhibited directly at the pituitary level. Through this same mechanism, decreasing cortisol levels lead to elevated ACTH levels. Biologically active ACTH results from enzymatic cleavage of a large precursor molecule, pro-opiomelanocortin (POMC). This molecule contains within its structure the amino acid sequences of ACTH, Pro-ACTH, -melanocyte stimulating hormone, lipotropin, as well as endorphin and the enkephalins. Because the reaction in immunoassays is determined by antigenic structure, not biological function, the usual ACTH RIA reacts with POMC, Pro-ACTH, ACTH and some fragments of the ACTH. ACTH may also be referred to by the following terms: ACTH ELISA kit, Adrenocorticotropic Hormone ELISA kit, Adrenocorticotropin ELISA kit, Alpha-Melanocyte-Stimulating Hormone ELISA kit, Alpha-MSH ELISA kit, Beta-Endorphin ELISA kit, Beta-LPH ELISA kit, Beta-Melanocyte-Stimulating Hormone ELISA kit, Beta-MSH ELISA kit, CLIP ELISA kit, Corticotropin-like Intermediary Peptide ELISA kit, Corticotropin-Lipotropin ELISA kit, Corticotropin-Lipotropin ELISA kit, Gamma-LPH ELISA kit, Gamma-MSH ELISA kit, Lipotropin Beta ELISA kit, Lipotropin Gamma ELISA kit, LPH ELISA kit, Melanotropin Alpha ELISA kit, Melanotropin Beta ELISA kit, Melanotropin Gamma ELISA kit, Met-Enkephalin ELISA kit, MSH ELISA kit, NPP ELISA kit, POC ELISA kit, Pro-ACTH-endorphin ELISA kit, Pro-OpioMelanocortin ELISA kit, ProopioMelanocortin ELISA kit, ProopioMelanocortin PreProProtein ELISA kit

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• RRID
• 2 years ago - submitted by Viktoria Fisi

The Metabolomics, Lipidomics, and Proteomics Platform facilitates metabolism-related and molecular research, serving researchers from academia and industry in Luxembourg and beyond. We specialize in the comprehensive analysis of small molecules and proteins within complex biological matrices. Our advanced analytical infrastructure and broad method portfolio accelerate scientific discoveries across biology, biomedicine, and systems biology.

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• SciCrunch
• 2 years ago - submitted by Edyta Vieth

Software tool that quantifies tumor content in cfDNA from 0.1× coverage whole-genome sequencing data without prior knowledge of tumor mutations. Used to simultaneously segment genome, predict large scale copy number alterations, and estimate tumor fraction of ultra low pass whole genome sequencing sample.

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• RRID
• 2 years ago - by Anonymous

Software tool for annotating fusion transcripts, incorporated into Trinity Cancer Transcriptome Analysis Toolkit utilities, and leveraging databases including CTAT Human Fusion Lib.

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• RRID
• 2 years ago - by Anonymous

Software application to estimate presence of MRD in plasma cfDNA WGS through evaluation of matched tumour-derived mutations (SNVs or CNVs).

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• RRID
• 2 years ago - by Anonymous

Software tool for microsatellite instability detection using high throughput sequencing data. It accepts the whole genome sequencing, whole exome sequencing and target region sequencing data as input. Introduces multinomial distribution model to quantify polymerase slippages for each tumor sample and discriminative sites selection method to enable MSI detection without matched normal samples.

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• RRID
• 2 years ago - by Anonymous

Open source platform to support management of educational audio and video content. Institutions can use Opencast to produce lecture recordings, manage existing video, serve designated distribution channels, and provide user interfaces to engage students with educational videos.

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• RRID
• 2 years ago - submitted by Manuel Oellers

Core utilizes dual tube micro X-ray computed tomography scanner for imaging specimens in fields of paleontology, comparative anatomy, evolution and development, basic biomedical studies, and research in geology and material sciences.It is equipped with 180 kV nano-focus and high power 240 kV micro-focus CT tube. This setup allows to scan very small specimens with high resolution using nano tube or dense fossils using high power tube.

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• SciCrunch
• 2 years ago - by Anonymous

Core provides bioinformatics and statistics expertise. Core develops and maintains automated and customizable workflows for processing and analyzing many types of genomic data; supports design of rigorous, robust and reproducible experiments and proposals; assists in preparation of manuscripts, grants and presentations; and provides education and training in use of software and analysis methods.Core can contribute to many types of bioinformatics or statistical projects.

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• SciCrunch
• 2 years ago - submitted by Edyta Vieth

Core is part of the University of Manchester Faculty of Science and Engineering. Facility offers support and services in chemistry, biology and materials. Mass spectrometers including Orbitraps, Ion Mobility, MALDI, DESI, hyphenated LC-MS and GC-MS apparatuses. Other instruments are HPLC systems, UHPLC systems and GC systems.

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• RRID
• 2 years ago - by Anonymous