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Dynabeads™ Mouse T Activator CD3/CD28 provides a simple method for T cell activation and expansion without the need for feeder layer cells (antigen-presenting cells) or antigens. The 4.5 µm diameter inert superparamagnetic beads are similar in size to antigen-presenting cells and are covalently coupled with anti-CD3 and anti-CD28 antibodies. These two antibodies provide the primary signal and co-stimulatory signal, optimized for efficient T cell activation and expansion. Recombinant IL-2 can be used to stimulate the expansion of T cell populations. After activation or expansion, the magnetic beads can be easily removed using a DynaMag™ magnet.

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• 2 years ago - submitted by Yujing Zhong

CFSE Cell Division Tracker Kit is composed of 5 vials, 100 µg per vial of CFSE (formally known as 5-(and 6)-Carboxyfluorescein diacetate succinimidyl ester of CFDA SE), and 500 µl of anhydrous DMSO. CFSE is able to passively diffuse into cells. Inside the cell, its acetate groups are cleaved by intracellular esterases, and the molecules are converted to fluorescent esters. CFSE is retained within the cell and covalently couples to intracellular molecules via its succinimidyl group. Due to this covalent coupling reaction, fluorescent CFSE can be retained within the cell for an extremely long period. Also, due to this stable linkage, once the dye has been incorporated within the cell, it is not transferred to adjacent cells. CFSE is widely used for cell proliferation assays and in vivo cell tracking.

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• 2 years ago - submitted by Yujing Zhong

Mouse CD8a (Ly-2) MicroBeads have been developed for positive selection or depletion of mouse CD8a+ T cells from single-cell suspensions of lymphoid and non-lymphoid tissues or from peripheral blood.

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• 2 years ago - submitted by Yujing Zhong

M-CSF, also known as CSF-1, is a four-α-helical-bundle cytokine that is the primary regulator of macrophage survival, proliferation and differentiation (1-3). M-CSF protein is also essential for the survival and proliferation of osteoclast progenitors (1, 4). M-CSF also primes and enhances macrophage killing of tumor cells and microorganisms, regulates the release of cytokines and other inflammatory modulators from macrophages, and stimulates pinocytosis (2, 3). M-CSF increases during pregnancy to support implantation and growth of the decidua and placenta (5). Sources of M-CSF include fibroblasts, activated macrophages, endometrial secretory epithelium, bone marrow stromal cells and activated endothelial cells (1-5). The M-CSF receptor (c-fms) transduces its pleotropic effects and mediates its endocytosis. M-CSF mRNAs of various sizes occur (3-9). Full length mouse M-CSF transcripts encode a 520 amino acid (aa) type I transmembrane (TM) protein with a 462 aa extracellular region, a 21 aa TM domain, and a 37 aa cytoplasmic tail that forms a 140 kDa covalent dimer. Differential processing produces two proteolytically cleaved, secreted dimers. One is an N- and O- glycosylated 86 kDa dimer, while the other is modified by both glycosylation and chondroitin-sulfate proteoglycan (PG) to generate a 200 kDa subunit. Although PG-modified M-CSF protein can circulate, it may be immobilized by attachment to type V collagen (8). Shorter transcripts encode M-CSF that lacks cleavage and PG sites and produces an N-glycosylated 68 kDa TM dimer and a slowly produced 44 kDa secreted dimer (7). Although forms may vary in activity and half-life, all contain the N-terminal 150 aa portion that is necessary and sufficient for interaction with the M-CSF receptor (10, 11). The first 229 aa of mature mouse M-CSF shares 87%, 83%, 82% and 81% aa identity with corresponding regions of rat, dog, cow and human M-CSF, respectively (12, 13). Human M-CSF is active in the mouse, but mouse M-CSF is reported to be species-specific.

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• 2 years ago - submitted by Yujing Zhong

RPMI 1640 medium (Roswell Park Memorial Institute 1640 medium) was initially developed for the suspension culture of human leukemia monolayer cells. RPMI 1640 has been found to be suitable for a variety of mammalian cells, including HeLa, Jurkat, MCF-7, PC12, PBMCs, astrocytes, and cancer cells.

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• 2 years ago - submitted by Yujing Zhong

Lipofectamine 3000 is a widely used transfection reagent primarily designed for the efficient delivery of nucleic acids (such as DNA and RNA) into cells. Here are some detailed descriptions of Lipofectamine 3000: Composition: Lipofectamine 3000 is a lipid-based transfection reagent that contains various cationic lipids and polymers, enabling the formation of complexes with nucleic acids to facilitate their penetration through the cell membrane. Transfection Efficiency: Compared to other transfection reagents, Lipofectamine 3000 generally exhibits higher transfection efficiency, effectively transfecting a wide range of cell types, including those that are typically difficult to transfect. Versatility: It is suitable for various applications, such as gene expression studies, RNA interference (RNAi), and CRISPR/Cas9 gene editing. It supports the transfection of different types of nucleic acids, including plasmid DNA, mRNA, and siRNA. Cell Types: Lipofectamine 3000 is effective for a variety of cells, including cell lines and primary cells, particularly demonstrating excellent performance in the transfection of mammalian cells. Optimization: The reagent provides a range of optimization conditions to help users adjust transfection efficiency based on specific cell types and experimental requirements. Usage Method: Typically, in the laboratory, Lipofectamine 3000 is mixed with nucleic acids to form lipid complexes, which are then added to the cell culture medium for transfection.

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• 2 years ago - submitted by Yujing Zhong

Hygromycin B is a broad-spectrum antibiotic primarily used for selective resistance and cell culture. It was originally isolated from the bacterium Streptomyces hygroscopicus. Here are some detailed descriptions of Hygromycin B: Mechanism of Action: Hygromycin B exerts its effects by interfering with bacterial protein synthesis. It binds to the ribosomal subunit, inhibiting the elongation of the peptide chain, which leads to a blockade of protein synthesis. Resistance Mechanism: Some bacteria and eukaryotic cells can develop resistance to Hygromycin B through specific genes (such as the hyg gene), allowing them to survive in environments where the antibiotic is present. Applications: Molecular Biology: Used for selecting cells that express specific resistance genes in transgenic plants and animal cells. Microbiology: Employed to select resistant bacteria for studying specific genetic backgrounds or resistance mechanisms. Cytotoxicity: Although Hygromycin B is widely used in cell culture, it has a certain level of cytotoxicity to mammalian cells, so careful concentration control is necessary when using it. Dosage and Usage: It is typically added to cell culture at specific concentrations (e.g., 50-400 µg/mL), depending on the cell type and experimental design. Overall, Hygromycin B is an important research tool widely used in molecular biology and cell biology.

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• 2 years ago - submitted by Yujing Zhong

MycoAlert detection kit

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• 2 years ago - submitted by Yujing Zhong

antibiotic

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• 2 years ago - submitted by Yujing Zhong

Fetal Bovine Serum

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• 2 years ago - submitted by Yujing Zhong

cell culture medium

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• 2 years ago - submitted by Yujing Zhong

cell culture medium

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• 2 years ago - submitted by Yujing Zhong

Web server that integrated data visualization and graphing functions together. Can be run easily with all Web browsers, with user-friendly graphical interface, users can paste your data directly into input box according to defined file format. Resulting graphs can be downloaded in bitmap (PNG or TIFF) or vector (PDF or SVG) format in publication quality. Used for data visualization and graphing.

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• 2 years ago - by Anonymous

Software tool for differential cell-cell communication analysis from multi-sample multi-condition single-cell transcriptomics data.

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• 2 years ago - by Anonymous

Data about Alabama, Maryland, Missouri and Connecticut people and economy.

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• 2 years ago - by Anonymous

Provides services for data analysis, pipeline management, and artificial intelligence research and operations. Data types analysed by the core include whole-genome and whole-exome sequencing, targeted re-sequencing, radiological images, RNA-sequencing, single-cell RNA sequencing, ChIP-sequencing, NanoString and various types of microarray data. Core also develops and maintains software infrastructure required for bioinformatics processing, including pipelines and cloud provision systems.

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• SciCrunch
• 2 years ago - submitted by Edyta Vieth

Siemens Biograph mMR represents major technological breakthrough in imaging field by combining positron emission tomography (PET) and magnetic resonance imaging (MRI) in one scanner. This system allows simultaneous acquisition of MR and PET images in human subjects.

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• 2 years ago - submitted by Letizia Amadori

System composed of DNBseq PE150 sequencing platform for RNA sequencing transcriptome with BGISEQ-500 desktop sequencer which uses DNA nanoball and combinational probe anchor synthesis developed from Complete Genomics sequencing technologies to generate short reads at large scale. DNBseq PE150 sequencing platform uses DNA nanoballs that are loaded onto flow cells and then read using fluorescent probes. The platform then uses lasers to excite the probes and take millions of images to identify bases.

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• 2 years ago - submitted by Letizia Amadori

Analysis Applications Hybrid Cell Count insufficient information letter sent

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• 2 years ago - submitted by Letizia Amadori

BZ-X800 Analyzer software to analyze and view images acquired with the BZ-X800 microscope.

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• 2 years ago - submitted by Letizia Amadori