URL: http://www.addgene.org/13367
Proper Citation: RRID:Addgene_13367
Insert Name: SRY-box containing gene 2
Organism: Mus musculus
Bacterial Resistance: Ampicillin
Defining Citation: PMID:16904174
Vector Backbone Description: Backbone Size:4600; Vector Backbone:pMXs; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Comments: The sequence of primer pMX-S1811 is GAC GGC ATC GCA GCT TGG ATA CAC. The Sox2 cDNA contains a modified ATG start codon to TTG for cloning into vectors with an N-terminal tag. If this cDNA is used in a non-tagged construct, a functional protein (missing the first 3 amino acids--MYN) is produced as confirmed by co-immunoprecipitation, reporter assay, and other experiments.
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Source: Addgene