URL: http://www.addgene.org/13367
Proper Citation: RRID:Addgene_13367
Insert Name: SRY-box containing gene 2
Organism: Mus musculus
Bacterial Resistance: Ampicillin
Defining Citation: PMID:16904174
Vector Backbone Description: Backbone Size:4600; Vector Backbone:pMXs; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Comments: The sequence of primer pMX-S1811 is GAC GGC ATC GCA GCT TGG ATA CAC. The Sox2 cDNA contains a modified ATG start codon to TTG for cloning into vectors with an N-terminal tag. If this cDNA is used in a non-tagged construct, a functional protein (missing the first 3 amino acids--MYN) is produced as confirmed by co-immunoprecipitation, reporter assay, and other experiments.
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Source: Addgene (RRID:SCR_002037)
Description: Non-profit plasmid repository dedicated to helping scientists around the world share high-quality plasmids. Facilitates archiving and distributing DNA-based research reagents and associated data to scientists worldwide. Repository contains over 65,000 plasmids, including special collections on CRISPR, fluorescent proteins, and ready-to-use viral preparations. There is no cost for scientists to deposit plasmids, which saves time and money associated with shipping plasmids themselves. All plasmids are fully sequenced for validation and sequencing data is openly available. We handle the appropriate Material Transfer Agreements (MTA) with institutions, facilitating open exchange and offering intellectual property and liability protection for depositing scientists. Furthermore, we curate free educational resources for the scientific community including a blog, eBooks, video protocols, and detailed molecular biology resources.
URL: http://www.addgene.org
