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Antibody Name
RRID:AB_572215 RRID Copied  
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(ImmunoStar Cat# 24446, RRID:AB_572215)
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Antibody Information

URL: http://antibodyregistry.org/AB_572215

Proper Citation: (ImmunoStar Cat# 24446, RRID:AB_572215)

Target Antigen: 5-HTP

Host Organism: rabbit

Clonality: polyclonal

Comments: Manufacturer Applications: Immunohistochemistry, Immunocytochemistry, Immunofluoresence, Western Blot; Note, The 5-HT Antibody was raised in rabbit against 5-HTP coupled to BSA with paraformaldehyde. The antibody has a proven maximum biotin-streptavidin/HRP staining at a 1/1000 - 1/2000 dilution in rat raphe nuclei. Optimal dilution will vary depending upon fixation, labeling technique and/or detection system; therefore, a dilution series is recommended. The specificity of the antiserum was evaluated using a model system of gelatin-indole plugs by a method similar to published procedures (Shipper and Tilders, 1983). Results showed that the 5-HTP antibody dose dependently stained 5-HTP but did not stain any concentration of 5-HT or 5-HIAA. The antiserum was also tested by pre-adsorption with indole/paraformaldehyde/BSA conjugates. Staining was completely blocked by pre-adsorption with 5-HTP conjugate and unaffected by 5-HIAA or 5-HT conjugate.; Gene Symbol: Avp

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