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Antibody Name
RRID:AB_2883968 RRID Copied  
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(Takeda Pharmaceutical Cat# P2L-1T, RRID:AB_2883968)
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Antibody Information

URL: http://antibodyregistry.org/AB_2883968

Proper Citation: (Takeda Pharmaceutical Cat# P2L-1T, RRID:AB_2883968)

Target Antigen: Prolactin-releasing peptide

Host Organism: mouse

Clonality: monoclonal

Comments: Permission of Takeda Pharmaceutical Company is necessary for the distribution of anti-PrRP mAb (P2L-1C).
"Region-specific monoclonal antibodies, P2L-1C and P2L-1T, which recognize the C-terminal and the internal sequence of PrRP, respectively: [Cys17]hPrRP-(17–31)amide and[ Cys25]hPrRP-(12–25)amide were used for immunogens. Conjugation of 1.7 mmol of these immunogens with 30 nmol bovine thyroglobulin previously maleimidated with N-(γ-maleimidobutyryloxy)succinimide was performed. These immunogens (40 mg/mouse) together with complete or incomplete Freund’s adjuvant were sc injected into BALB/c mice (female, 8 weeks old) at 3-week intervals. Four days after iv injecting each mouse with 200 mg of the immunogen, spleen cells were separated and fused with mouse myeloma cells (P3-X63Ag8-U1) as described previously (9). Monoclonal antibodies, P2L-1T (IgG2a, κ) and P2L-1C (IgG2a, κ) were selected and purified from ascites fluid with a protein A-immobilized column"

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