URL: http://antibodyregistry.org/AB_219374
Proper Citation: (Rockland Cat# 600-401-383, RRID:AB_219374)
Target Antigen: FLAG
Host Organism: rabbit
Clonality: polyclonal
Comments: This antibody is optimally suited for monitoring the expression of FLAGtagged fusion proteins. As such, this antibody can be used to identify fusion proteins containing the FLAGepitope. The antibody recognizes the epitope tag fused to either the amino- or carboxy- termini of targeted proteins. This antibody has been tested by ELISA and western blotting against both the immunizing peptide and FLAG containing recombinant proteins. Although not tested, this antibody is likely functional for immunoprecipitation, immunocytochemistry, and other immunodetection techniques. The epitope tag peptide sequence was first derived from the 11-amino-acid leader peptide of the gene-10 product from bacteriophage T7. Now the most commonly used hydrophilic octapeptide is DYKDDDDK. Rockland Immunochemical's polyclonal antibody to detect FLAGconjugated proteins binds FLAGcontaining fusion proteins with greater affinity than the widely used monoclonal M1, M2 and M5 clones, and shows greater sensitivity in most assays. Affinity purification of the polyclonal antibody results in very low background levels in assays and low cross-reactivity with other cellular proteins. Applications: ELISA,IHC Frozen,IHC Paraffin,WB Dilution: IHC -User Optimized / WB -1:2,000 - 1:10,000
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Source: Antibody Registry
