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http://www.scienceexchange.com/facilities/transgenic-mouse-shared-resource-columbia

THIS RESOURCE IS NO LONGER IN SERVICE. Documented on April 22, 2024. Core facility that specializes in the generation and analysis of genetically modified mice, including transgenic and knockout animals, is an essential technology for biomedical research. Our services include: Production of transgenic mice by pronuclear DNA microinjection and embryo transfer. Production of chimeric mice by blastocyst injection of genetically modified ES cells and embryo transfer, which is part of the procedure of producing knockout or knockin mice. Gene targeting, which involves the electroporation of a targeting vector into pluripotent mouse embryonic stem (ES) cells and the selection of gene-targeted clones. Education of investigators and advice on vector and experimental design for experiments using transgenic and knockout mice. Bioimaging: The Animal Imaging Service provides investigators the opportunity for non-invasive detection, localization, and longitudinal monitoring of primary and metastatic cancer cells in vivo, in allograft, zenograft, transgenic and knock-out mouse models. The IVIS Spectrum (Caliper Life Sciences, Hopkinton MA) uses optical imaging technology to detect bioluminescent and fluorescent reporters across the blue to near infrared wavelength region. DNA samples and ES cell lines are injected in the order in which the signed service request forms are received. Currently, the average time from receipt of the service request to injection is approximately 2 weeks. For ES Cell electroporation and expansion, success in gene targeting cannot be guaranteed due to inherent variability in the frequency of targeting different loci, and the possibility of mistakes or mutations in the targeting vector.

Proper citation: Columbia Transgenic Mouse Shared Resource (RRID:SCR_012640) Copy   

•   Source: SciCrunch Registry

http://corefacilities.case.edu/animal.php

A set of core facilities of Case Western Reserve University School of Medicine which allows users to create and analyze in vivo animal models. The various facilities provide animal care, transgenic models, imaging, irradiation, and phenotyping for research concerning such topics as cancer, metabolic processes, and behavior. In vivo animals provided include mice, zebrafish, and rodents.

Proper citation: CWRU In Vivo Animal Facilities (RRID:SCR_014209) Copy   

•   Source: SciCrunch Registry

http://drc.ucsf.edu/mouse-metabolism-core

Core which provides technical support for UCSF investigators to conduct metabolic studies using a 12-chambered Comprehensive Lab Animal Monitoring System (CLAMS), an EchoMRI, and Dual energy X-ray absorptiometry, which together allow measurement of food intake, water intake, motor activities, core temperature, and body composition in live mice. It also helps to identify emerging technologies that will enhance multiple research programs and coordinates the acquisition and maintenance of those facilities.

Proper citation: University of California San Francisco Diabetes Research Center Mouse Metabolism Core (RRID:SCR_015101) Copy   

•   Source: SciCrunch Registry

http://diabetesresearchcenter.dom.wustl.edu/translational-diagnostics-core/

Core facility which provides assays for hormones and metabolites for the study of diabetes and related metabolic disorders.

Proper citation: Washington University School of Medicine Diabetes Research Center Immunoassay Core (RRID:SCR_015161) Copy   

•   Source: SciCrunch Registry

http://diabetesresearchcenter.dom.wustl.edu/transgenic-es-cell-core/

Core facility that provides services for efficient and cost-effective development of genetically altered mouse models to elucidate the pathogenesis of diabetes and related metabolic disorders.

Proper citation: Washington University School of Medicine Diabetes Research Center Transgenic and ES Cell Core (RRID:SCR_015141) Copy   

•   Source: SciCrunch Registry

https://www.derc.cumc.columbia.edu/core-facilities-and-services/mouse-metabolic-function-and-phenotyping-core

Core that provides services that facilitate the efficient characterization of mouse models of diabetes and its complications: NMR Body Composition Analysis, Whole Body Metabolic Assessment (chamber calorimetry with motion detection), Metabolic Clamps, Gastric Infusion/Feeding and Thermogenic Phenotyping.

Proper citation: Columbia Diabetes Research Center Mouse Metabolic Function and Phenotyping Core (RRID:SCR_015082) Copy   

•   Source: SciCrunch Registry

http://www.hopkinsmedicine.org/diabetes-research-center/research-cores/cell-biology.html

Core that provides services in tissue analysis and imaging, islet biology, liver biology, and adipocyte biology. It includes the indepedent cores Derivation of IPSC and Bioenergetics as well as the Specialty Animal Surgery Subcore and Bone Biology Subcore.

Proper citation: Johns Hopkins University - University of Maryland Diabetes Research Center Cell Biology Core (RRID:SCR_015090) Copy   

•   Source: SciCrunch Registry

http://drtc.bsd.uchicago.edu/islet-cell-biology-core-about/

Core which provides services and hands-on training in the isolation and functional characterization of pancreatic islets from normal and diabetic humans and mice. It also maintains a repository of insulinoma cell lines for distribution. It puts emphasis on facilitating studies of primary islet cells and it has developed many unique tools and techniques for carrying such studies including novel animals models, biophysical methods and a library of adenovirus-based expression constructs for studying beta-cell function.

Proper citation: University of Chicago Diabetes Research and Training Center Islet Cell Biology Core (RRID:SCR_015132) Copy   

•   Source: SciCrunch Registry

http://depts.washington.edu/diabetes/viral-vector-and-transgenic-mouse/

Core facility that provides Diabetes Research Center affiliates with vectors necessary to overexpress, knockdown, knockout, or alter expression of RNAs and proteins of interest in cultured cells, isolated tissues, and animals.

Proper citation: University of Washington Diabetes Research Center Vector and Transgenic Mouse Core (RRID:SCR_015130) Copy   

•   Source: SciCrunch Registry

http://www.massgeneral.org/csibd/cores/genetic.aspx

Core whose services include breeding and maintenance of mutant mouse stocks, characterization of murine models, bone marrow transfers and adoptive T cell transfers, in vivo tracking of fluorescently labeled bacteria and cells, and whole body imaging of bioluminescent and fluorescent signals.

Proper citation: Center for the Study of Inflammatory Bowel Disease Genetic Animal Models Core (RRID:SCR_015238) Copy   

•   Source: SciCrunch Registry

http://obriencenter.yale.edu/mousecelllines.aspx

Core provides services to generate unique in vivo mouse models and kidney specific cell lines.

Proper citation: George M. O'Brien Kidney Center at Yale Mouse Genetics and Cell Line Core (RRID:SCR_015299) Copy   

•   Source: SciCrunch Registry

https://ddrcc.wustl.edu/scientific-cores/murine-models-gnotobiotics-core/

Core facility that provides advice and technical services to DDRCC investigators that include production of transgenic and chimeric mice, derivation of speed congenics and assisted reproduction services for maintaining pedigrees via the Mouse Genetics Core, as well as gnotobiotic mouse provision, colonization and rederivation.

Proper citation: Washington University Digestive Diseases Research Core Center Murine Models and Gnotobiotics Core (RRID:SCR_015260) Copy   

•   Source: SciCrunch Registry

http://livercenter.yale.edu/core-facilities/cellular-molecular-physiology-core-facility.aspx

Core facility that provides technical expertise, equipment and personnel to Liver Center Investigators who wish to work with animal models of liver disease, isolated liver cells, or gene expression in liver tissue. The Cell Isolation sub-core isolates hepatocytes and non-parenchymal liver cells primarily from rat and mouse, while the Molecular Biology sub-core provides equipment and expertise to Liver Center members in a centralized facility.

Proper citation: Yale Liver Center Cellular and Molecular Physiology Core (RRID:SCR_015258) Copy   

•   Source: SciCrunch Registry

http://animalcare.umich.edu/business-services/germ-free-mouse-facility

Core that provides services to the University of Michigan community and can also ship germ-free mice to locations worldwide. Its projects include studies investigating inflammatory bowel disease and cancer, host microbiome interactions, infectious disease pathogenesis, and mammalian and bacterial metabolism.

Proper citation: MMPC-University of Michigan Medical School Animal Care and Germ-Free Mouse Core (RRID:SCR_015344) Copy   

•   Source: SciCrunch Registry

http://medicine.yale.edu/labmed/ycceh/cores/core_d/index.aspx

Core facility which provides expertise, technical assistance, and mice for human-into-mouse xenotransplantation studies. Mouse models include MITRG-SKI (KnockIn) mice that express human cytokines and huSIRPa from the endogenous murine loci in the Rag-/- IL-2Ry -/- background. It also offers training and technical assistance in the study of hematopoiesis and benign hematologic questions in mice.

Proper citation: Yale Cooperative Center of Excellence in Hematology Animal Modeling Core (RRID:SCR_015349) Copy   

•   Source: SciCrunch Registry

http://zfrhmaps.tch.harvard.edu/cemh/CoreC.htm

Core facility for basic and translational stem cell research. The core's areas of expertise include human pluripotent stem cell biology, cGMP cell manufacturing, reprogramming, genome editing, genotyping, laboratory automation, chemical screening, and imaging/image analysis.

Proper citation: Boston Children's Hospital Center of Excellence in Molecular Hematology Stem Cell Engineering and Analysis Core (RRID:SCR_015352) Copy   

•   Source: SciCrunch Registry

https://labnodes.vanderbilt.edu/resource/view/id/10800/community_id/1418

THIS RESOURCE IS NO LONGER IN SERVICE. Documented on July 6th,2023. Core whose objective is to provide investigators at Vanderbilt and outside institutions a means to accurately assess cardiovascular phenotypes in mouse models of diabetes and metabolic disease. The CPC uses validated approaches and state-of-the-art instrumentation that allow for sensitive screening of phenotypic variations.

Proper citation: MMPC-Vanderbilt University School of Medicine Cardiovascular Pathophysiology Core (RRID:SCR_015353) Copy   

•   Source: SciCrunch Registry

https://www.med.unc.edu/marsicolunginstitute/core-facilities/mousemodelscore

Core that provides support and expertise for mouse colony maintenance, breeding strategies, mouse lung phenotyping protocols, and submission of live-animal applications to maintain compliance with IACUC guidelines.

Proper citation: Cystic Fibrosis and Pulmonary Diseases Research and Treatment Center Mouse Models Core (RRID:SCR_015389) Copy   

•   Source: SciCrunch Registry

https://labnodes.vanderbilt.edu/resource/view/id/10822/community_id/1418

Core that ensures the health and welfare of mouse models by assuring that mice admitted to the MMPC meet defined health criteria and that post-quarantine protocol assignment. It diagnoses disease and identifies unique metabolic or cardiovascular phenotypes of mice through interaction with the Comparative Pathology Subcore.

Proper citation: MMPC-Vanderbilt University School of Medicine Animal Health and Welfare Core (RRID:SCR_015380) Copy   

•   Source: SciCrunch Registry

https://www.uab.edu/medicine/cysticfibrosis/about/animal-core

Core that assists in mouse line generation by both oocyte micro-injection and by embryonic stem cell gene targeting technologies. It also establishes breeding colonies of cystic fibrosis mice, and genotypes and provides these to UAB investigators and furnishes electrophysiology tests of CFTR activation, including murine nasal potential difference assays and intestinal short circuit current measurements.

Proper citation: Gregory Fleming James Cystic Fibrosis Research Center Cystic Fibrosis Animal Models Core (RRID:SCR_015396) Copy   

•   Source: SciCrunch Registry