Alphaviruses are a large group of re-emerging arthropod-borne RNA viruses. The compact viral RNA genomes harbor diverse structures that facilitate replication. These structures can be recognized by antiviral cellular RNA-binding proteins, including DExD-box (DDX) helicases, that bind viral RNAs to control infection. The full spectrum of antiviral DDXs and the structures that are recognized remain unclear. Genetic screening identified DDX39A as antiviral against the alphavirus chikungunya virus (CHIKV) and other medically relevant alphaviruses. Upon infection, the predominantly nuclear DDX39A accumulates in the cytoplasm inhibiting alphavirus replication, independent of the canonical interferon pathway. Biochemically, DDX39A binds to CHIKV genomic RNA, interacting with the 5' conserved sequence element (5'CSE), which is essential for the antiviral activity of DDX39A. Altogether, DDX39A relocalization and binding to a conserved structural element in the alphavirus genomic RNA attenuates infection, revealing a previously unknown layer to the cellular control of infection.
Pubmed ID: 37949067 RIS Download
Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.
This polyclonal targets Rck / p54
View all literature mentionsThis polyclonal targets Cow Histone H3
View all literature mentionsThis monoclonal targets alpha-Tubulin
View all literature mentionsThis monoclonal targets Aly
View all literature mentionsThis monoclonal targets GADPH
View all literature mentionsThis monoclonal targets TIAR
View all literature mentionsThis polyclonal targets DDX39B
View all literature mentionsThis unknown targets Streptavidin-HRP
View all literature mentionsThis polyclonal targets DDX24
View all literature mentionsThis monoclonal targets DDX39 monoclonal antibody clone 2E4
View all literature mentionsThis monoclonal targets Influenza A Virus Nucleoprotein
View all literature mentionsThis recombinant monoclonal targets dsRNA
View all literature mentionsThis monoclonal targets β-Actin
View all literature mentionsThis monoclonal targets BAT1/DDX39 (H-6)
View all literature mentionsThis polyclonal targets LMNB1
View all literature mentionsThis polyclonal targets DDX39A
View all literature mentionsCell line Vero is a Spontaneously immortalized cell line with a species of origin Chlorocebus sabaeus
View all literature mentionsCell line Vero C1008 is a Spontaneously immortalized cell line with a species of origin Chlorocebus sabaeus
View all literature mentionsCell line HEK293T is a Transformed cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line HeLa S3 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line A-549 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line U2OS is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentions