The treatment of depression with pharmaceuticals is associated with many adverse side effects, including male fertility problems. The precise mechanisms by which these agents affect testicular cells remain largely unknown, but they are believed to induce cellular stress, which is sensed by the endoplasmic reticulum (ER) and the Golgi apparatus. These organelles are responsible for maintaining cellular homeostasis and regulating signal pathways that lead to autophagy or apoptosis. Therefore, in this study, we aimed to investigate the autophagy, ER, and Golgi stress-related pathways in mouse testis following treatment with antidepressant-like substances (ALS) and ALS combined with lipopolysaccharide (LPS). We found that most ALS and activated proteins are associated with the induction of apoptosis. However, when imipramine (IMI) was combined with NS-398 (a cyclooxygenase-2 inhibitor) after LPS administration, we observed a marked increase in the BECLIN1, Bcl-2, ATG16L, and LC3 expression, which are marker proteins of autophagosome formation. The expression of the BECN1 and ATG16L genes was also high compared to the control, indicating the induction of autophagy processes that may potentially protect mouse testicular cells from death and regulate metabolism in the testis. Our findings may provide a better understanding of the stress-related effects of specific ALS on the testis. Video Abstract.
Pubmed ID: 37735683 RIS Download
Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.
Mus musculus with name C57BL/6J from IMSR.
View all literature mentionsThis polyclonal targets Mouse IgG (whole molecule)-Peroxidase antibody produced in rabbit
View all literature mentionsThis unknown targets Rabbit IgG, whole molecule
View all literature mentionsThis monoclonal targets Phospho-EIF2S1 (Ser52)
View all literature mentionsThis unknown targets Phospho-CHOP (Ser30)
View all literature mentionsThis unknown targets Phospho-IRE1 alpha (Ser724)
View all literature mentionsThis unknown targets Phospho-ASK1 (Ser966)
View all literature mentionsThis unknown targets Phospho-ATF4 (Ser245)
View all literature mentionsThis unknown targets ATF6
View all literature mentionsThis unknown targets Phospho-PERK (Thr982)
View all literature mentionsThis unknown targets GRASP65
View all literature mentionsThis monoclonal targets ACBD3
View all literature mentionsThis unknown targets WIPI1
View all literature mentionsThis unknown targets GOLGB1
View all literature mentionsThis unknown targets GADD34
View all literature mentionsThis unknown targets TRAF2
View all literature mentionsThis unknown targets ST3GAL1
View all literature mentionsThis unknown targets ARF4
View all literature mentionsThis unknown targets CREB3L4
View all literature mentionsThis unknown targets SERPINH1
View all literature mentionsThis unknown targets Phospho-p38 MAPK (Tyr182)
View all literature mentionsThis unknown targets ATG5
View all literature mentionsThis unknown targets TFE3
View all literature mentionsThis monoclonal targets Bcl-2 (C-2)
View all literature mentionsThis unknown targets LC3A/LC3B
View all literature mentionsThis unknown targets ATG16L1
View all literature mentionsThis unknown targets ULK1 (denatured)
View all literature mentionsThis unknown targets ATG14
View all literature mentionsThis unknown targets mTOR
View all literature mentionsThis unknown targets beta Actin
View all literature mentionsThis unknown targets Beclin 1
View all literature mentionsThis unknown targets ATG13
View all literature mentions