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Components of Isolated Skeletal Muscle Differentiated Through Antibody Validation.

bioRxiv : the preprint server for biology | 2023

Isolation of skeletal muscles allows for the exploration of many complex diseases. Fibroblasts and myoblast play important roles in skeletal muscle morphology and function. However, skeletal muscles are complex and made up of many cellular populations and validation of these populations is highly important. Therefore, in this article, we discuss a comprehensive method to isolate mice skeletal muscle, create satellite cells for tissue culture, and use immunofluorescence to validate our approach.

Pubmed ID: 37292961 RIS Download

Research resources used in this publication

None found

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Antibodies used in this publication

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Associated grants

  • Agency: NCATS NIH HHS, United States
    Id: UL1 TR000445
  • Agency: NICHD NIH HHS, United States
    Id: R01 HD090061
  • Agency: NHLBI NIH HHS, United States
    Id: R25 HL106365
  • Agency: NCRR NIH HHS, United States
    Id: UL1 RR024975
  • Agency: NIDDK NIH HHS, United States
    Id: T32 DK007563
  • Agency: BLRD VA, United States
    Id: I01 BX005352
  • Agency: NIDDK NIH HHS, United States
    Id: P30 DK020593

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DSHB (tool)

RRID:SCR_013527

An antibody supplier which banks and distributes hybridomas and monoclonal antibodies for use in research. The bank includes antibodies against targets such as GFP, transcription factors, stem cells, and human.

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