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Neurovascular unit and barrier maturation rely on vascular basement membrane (vBM) composition. Laminins, a major vBM component, are crucial for these processes, yet the signaling pathway(s) that regulate their expression remain unknown. Here, we show that mural cells have active Wnt/β-catenin signaling during central nervous system development in mice. Bulk RNA sequencing and validation using postnatal day 10 and 14 wild-type versus adenomatosis polyposis coli downregulated 1 (Apcdd1-/-) mouse retinas revealed that Lama2 mRNA and protein levels are increased in mutant vasculature with higher Wnt/β-catenin signaling. Mural cells are the main source of Lama2, and Wnt/β-catenin activation induces Lama2 expression in mural cells in vitro. Markers of mature astrocytes, including aquaporin 4 (a water channel in astrocyte endfeet) and integrin-α6 (a laminin receptor), are upregulated in Apcdd1-/- retinas with higher Lama2 vBM deposition. Thus, the Wnt/β-catenin pathway regulates Lama2 expression in mural cells to promote neurovascular unit and barrier maturation.
Pubmed ID: 36098369 RIS Download
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This monoclonal targets Beta-actin
View all literature mentionsThis polyclonal targets Laminin antibody produced in rabbit
View all literature mentionsThis monoclonal targets Itga6
View all literature mentionsThis unknown targets Water Channel Aquaporin 4
View all literature mentionsThis monoclonal targets CD140a
View all literature mentionsThis polyclonal targets Glial Fibrillary Acidic Protein (GFAP)
View all literature mentionsThis polyclonal targets GFP
View all literature mentionsThis monoclonal targets LEF1 (C12A5) Rabbit mAb
View all literature mentionsThis polyclonal targets Mouse PDGF R beta
View all literature mentionsThis polyclonal targets CD31
View all literature mentionsThis polyclonal targets Caveolin 1 antibody - Caveolae Marker
View all literature mentionsThis unknown targets
View all literature mentionsThis monoclonal targets ITGA2
View all literature mentionsThis monoclonal targets ITGA6
View all literature mentionsThis monoclonal targets Laminin beta 1
View all literature mentionsThis monoclonal targets Laminin 2 alpha antibody [4H8-2]
View all literature mentionsThis polyclonal targets Escherichia coli beta Galactosidase
View all literature mentionsThis polyclonal targets NG2 Chondroitin Sulfate Proteoglycan
View all literature mentionsThis monoclonal targets 129/Sv mouse-derived endothelioma cell line tEnd.1
View all literature mentionsMus musculus with name B6.129S1(Cg)-Lama2tm1Eeng/J from IMSR.
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