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Matrix metalloproteinase 7 (MMP7) is involved in the degradation of extracellular matrix in disease processes and therefore plays an important role in host disease resistance/susceptibility. To better understanding the effects of porcine MMP7 (pMMP7) on piglets diarrhea trait, we characterized pMMP7 gene, identified genetic variations in pMMP7 and explored the relationship between pMMP7 polymorphisms and piglets diarrhea in Min pig and Landrace populations. The complete coding sequence of pMMP7 is 804 bp encoding a protein of 267 amino acids. Sequence alignment showed that the identity between pMMP7 and human MMP7 was approximately 80%. The expression of pMMP7 in the gut of healthy piglets were weak and the distribution of the pMMP7-EGFP fusion protein was observed mainly in the cytoplasm. After the identification of 21 genetic variations in 5' flanking region and exons, Hae III and Eco72 Ⅰ PCR-RFLP were established to genotype SNP rs327380117 and rs329429922, respectively. Statistical analysis indicated that Landrace piglets with a TT genotype at rs327380117 had a lower diarrhea score and day-14 wt than TC piglets (p < 0.05); the diarrhea score of AA Landrace animals with rs329429922 was lower than that of GG individuals (p < 0.05). The findings presented here contribute to the understanding of the biological function of pMMP7 and may provide new molecular markers for pig breeding.
Pubmed ID: 35182673 RIS Download
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Open source database of curated, non-redundant set of profiles derived from published collections of experimentally defined transcription factor binding sites for multicellular eukaryotes. Consists of open data access, non-redundancy and quality. JASPAR CORE is smaller set that is non-redundant and curated. Collection of transcription factor DNA-binding preferences, modeled as matrices. These can be converted into Position Weight Matrices (PWMs or PSSMs), used for scanning genomic sequences. Web interface for browsing, searching and subset selection, online sequence analysis utility and suite of programming tools for genome-wide and comparative genomic analysis of regulatory regions. New functions include clustering of matrix models by similarity, generation of random matrices by sampling from selected sets of existing models and a language-independent Web Service applications programming interface for matrix retrieval.
View all literature mentionsCentral online repository for microRNA nomenclature, sequence data, annotation and target prediction.Collection of published miRNA sequences and annotation.
View all literature mentionsDatabase of known and predicted protein interactions. The interactions include direct (physical) and indirect (functional) associations and are derived from four sources: Genomic Context, High-throughput experiments, (Conserved) Coexpression, and previous knowledge. STRING quantitatively integrates interaction data from these sources for a large number of organisms, and transfers information between these organisms where applicable. The database currently covers 5''214''234 proteins from 1133 organisms. (2013)
View all literature mentionsWeb sevice of ClustalW provided by DNA data bank of Japan.
View all literature mentionsCell line IPEC-J2 is a Spontaneously immortalized cell line with a species of origin Sus scrofa
View all literature mentionsThe SciCrunch Infrastructure was developed as a cooperative data platform to be used by diverse communities in making data more FAIR.
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