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Acute lung injury (ALI) is featured with severe lung edema at the early exudative phase, resulting from the imbalance of alveolar fluid turnover and clearance. Mesenchymal stem cells (MSCs) belong to multipotent stem cells, which have shown potential therapeutic effects during ALI. Of note, MSC-conditioned medium (MSC-CM) improved alveolar fluid clearance (AFC) in vivo, whereas the involvement of miRNAs is seldom known. We thus aim to explore the roles of miR-214 in facilitating MSC-CM mediated fluid resolution of impaired AFC. In this study, AFC was increased significantly by intratracheally administrated MSC-CM in lipopolysaccharide-treated mice. MSC-CM augmented amiloride-sensitive currents in intact H441 monolayers, and increased α-epithelial sodium channel (α-ENaC) expression level in H441 and mouse alveolar type 2 epithelial cells. Meanwhile, MSC-CM increased the expression of miR-214, which may participate in regulating ENaC expression and function. Our results suggested that MSC-CM enhanced AFC in ALI mice in vivo through a novel mechanism, involving miR-214 regulation of ENaC.
Pubmed ID: 34766129 RIS Download
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View all literature mentionsA set of useful accessory programs to the LINKAGE package. It simplifies the performance of a large array of parametric and nonparametric tests for linkage and association on data entered in LINKAGE format pedigree and parameter files. (entry from Genetic Analysis Software)
View all literature mentionsSoftware application for data analysis and graphing. Origin contains a variety of different graph types, including statistical plots, 2D and 3D vector graphs, and counter graphs. More advance version is OriginPro which offers advanced analysis tools and Apps for Peak Fitting, Surface Fitting, Statistics and Signal Processing.
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