Searching across hundreds of databases
Doublecortin-like kinase 1 (DCLK1) is critical for neurogenesis, but overexpression is also observed in multiple cancers and is associated with poor prognosis. Nevertheless, the function of DCLK1 in cancer, especially the context-dependent functions, are poorly understood. We present a "toolkit" that includes the DCLK1 inhibitor DCLK1-IN-1, a complementary DCLK1-IN-1-resistant mutation G532A, and kinase dead mutants D511N and D533N, which can be used to investigate signaling pathways regulated by DCLK1. Using a cancer cell line engineered to be DCLK1 dependent for growth and cell migration, we show that this toolkit can be used to discover associations between DCLK1 kinase activity and biological processes. In particular, we show an association between DCLK1 and RNA processing, including the identification of CDK11 as a potential substrate of DCLK1 using phosphoproteomics.
Pubmed ID: 32755567 RIS Download
Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.
Cell line DLD-1 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsThis monoclonal targets GAPDH
View all literature mentionsThis monoclonal targets DCAMKL1
View all literature mentionsCell line SUM159PT is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line Hs 578T is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line SUM1315MO2 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line UACC-893 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line CAMA-1 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line ZR-75-30 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line MDA-MB-361 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line MCF-12A is a Spontaneously immortalized cell line with a species of origin Homo sapiens
View all literature mentionsCell line HCC1143 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line ZR-75-1 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line EFM-19 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line CAL-85-1 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line 184B5 is a Transformed cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line SUM149PT is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line CAL-51 is a Cancer cell line with a species of origin Homo sapiens
View all literature mentionsCell line MCF-10A is a Spontaneously immortalized cell line with a species of origin Homo sapiens
View all literature mentionsCell line SUM52PE is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line 184A1 is a Transformed cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line CAL-120 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line HCC38 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line HCC1419 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line MDA-MB-175-VII is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line Evsa-T is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line HCC1395 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line HCC1569 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line BT-549 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line BT-474 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line MDA-MB-231 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line HCC70 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line HCC1937 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line BT-20 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line AU565 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line HCC202 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line MDA-MB-436 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line HCC1806 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line SUM185PE is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line MDA-MB-415 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line hTERT-HME1 is a Telomerase immortalized cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line HCC1954 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line SUM229PE is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line MDA-MB-330 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line T-47D is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line MDA-MB-468 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line UACC-812 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line MDA-MB-453 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line SK-BR-3 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsThe SciCrunch Infrastructure was developed as a cooperative data platform to be used by diverse communities in making data more FAIR.
scicrunch.org
