Developmental Therapeutics Program (tool)

RRID:SCR_003057

Portal for preclinical information and research materials, including web-accessible data and tools, NCI-60 Tumor Cell Line Screen, compounds in vials and plates, tumor cells, animals, and bulk drugs for investigational new drug (IND)-directed studies. DTP has been involved in the discovery or development of more than 70 percent of the anticancer therapeutics on the market today, and will continue helping the academic and private sectors to overcome various therapeutic development barriers, particularly through supporting high-risk projects and therapeutic development for rare cancers. Initially DTP made its drug discovery and development services and the results from the human tumor cell line assay publicly accessible to researchers worldwide. At first, the site offered in vitro human cell line data for a few thousand compounds and in vitro anti-HIV screening data for roughly 42,000 compounds. Today, visitors can find: * Downloadable in vitro human tumor cell line data for some 43,500 compounds and 15,000 natural product extracts * Results for 60,000 compounds evaluated in the yeast assay * In vivo animal model results for 30,000 compounds * 2-D and 3-D chemical structures for more than 200,000 compounds * Molecular target data, including characterizations for at least 1,200 targets, plus data from multiple cDNA microarray projects In addition to browsing DTP's databases and downloading data, researchers can request individual samples or sets of compounds on 96-well plates for research, or they can submit their own compounds for consideration for screening via DTP's online submission form. Once a compound is submitted for screening, researchers can follow its progress and retrieve data using a secure web interface. The NCI has collected information on almost half a million chemical structures in the past 50 years. DTP has made this information accessible and useful for investigators through its 3-D database, a collection of three-dimensional structures for more than 200,000 drugs. Investigators use the 3-D database to screen compounds for anticancer therapeutic activity. Also available on DTP's website are 127,000 connection tables for anticancer agents. A connection table is a convenient way of depicting molecular structures without relying on drawn chemical structures. As unique lists of atoms and their connections, the connection tables can be indexed and stored in computer databases where they can be used for patent searches, toxicology studies, and precursor searching, for example.

CD45.2-Biotin, mouse (antibody)

RRID:AB_2660733

This monoclonal targets CD45.2

Alexa Fluor(R) 647 anti-mouse CD85k (gp49 Receptor) (antibody)

RRID:AB_2562044

This monoclonal targets CD85k

CD117 (c-Kit) Monoclonal Antibody (2B8), FITC, eBioscience (antibody)

RRID:AB_465186

This monoclonal targets CD117 (c-Kit)

PE anti-human CD85k (ILT3) (antibody)

RRID:AB_2136645

This monoclonal targets CD85k

PE anti-human CD86 (antibody)

RRID:AB_2721632

This monoclonal targets CD86

FITC anti-human CD209 (DC-SIGN) (antibody)

RRID:AB_1134057

This monoclonal targets CD209

CD45.2 Monoclonal Antibody (104), PE, eBioscience (antibody)

RRID:AB_465679

This monoclonal targets CD45.2

CD85k (ILT3) Monoclonal Antibody (ZM4.1), APC, eBioscience (antibody)

RRID:AB_2043854

This monoclonal targets CD85k (ILT3)

CD45.1 Monoclonal Antibody (A20), APC, eBioscience (antibody)

RRID:AB_469398

This monoclonal targets CD45.1

CD15 Monoclonal Antibody (MMA), APC, eBioscience (antibody)

RRID:AB_2573137

This monoclonal targets CD15

CD14 Monoclonal Antibody (61D3), APC, eBioscience (antibody)

RRID:AB_10669167

This monoclonal targets CD14

CD11b Monoclonal Antibody (ICRF44), PE, eBioscience (antibody)

RRID:AB_2043799

This monoclonal targets CD11b

Anti-rabbit IgG (H+L), F(ab) 2 Fragment (Alexa Fluor 555 Conjugate) (antibody)

RRID:AB_10694110

This unknown targets rabbit IgG (H+L) F(ab) 2 Fragment

CD34 Monoclonal Antibody (4H11), FITC, eBioscience (antibody)

RRID:AB_1518732

This monoclonal targets CD34

BV786 Mouse Anti-Human CD45 (antibody)

RRID:AB_2716864

This monoclonal targets CD45

CD33 Monoclonal Antibody (HIM3-4), PE, eBioscience (antibody)

RRID:AB_10855031

This monoclonal targets CD33

Normal Mouse IgG (antibody)

RRID:AB_145840

This polyclonal secondary targets not applicable

Monoclonal ANTI-FLAG® M2 antibody (antibody)

RRID:AB_259529

This monoclonal targets FLAG

β-Actin (8H10D10) Mouse mAb (antibody)

RRID:AB_2242334

This monoclonal targets beta-actin

Goat Anti-Mouse IgG - H&L Polyclonal Antibody, HRP Conjugated (antibody)

RRID:AB_955439

This polyclonal targets Mouse Mouse IgG secondary - H&L

Goat Anti-Rabbit IgG - H&L Polyclonal antibody, Hrp Conjugated (antibody)

RRID:AB_955447

This polyclonal targets Rabbit Rabbit IgG secondary - H&L

GAPDH Antibody (0411) (antibody)

RRID:AB_627678

This monoclonal targets GAPDH

FTO antibody [EPR6895] (antibody)

RRID:AB_10972698

This monoclonal targets FTO antibody [EPR6895]

Anti-m6A (antibody)

RRID:AB_2279214

This polyclonal targets m6A

5-Hydroxymethylcytosine (5-hmC) antibody (antibody)

RRID:AB_10013602

This polyclonal targets 5-Hydroxymethylcytosine (5-hmC)

ZR-75-1 (cell line)

RRID:CVCL_0588

Cell line ZR-75-1 is a Cancer cell line with a species of origin Homo sapiens (Human)

ML-2 (cell line)

RRID:CVCL_1418

Cell line ML-2 is a Cancer cell line with a species of origin Homo sapiens (Human)

NOMO-1 (cell line)

RRID:CVCL_1609

Cell line NOMO-1 is a Cancer cell line with a species of origin Homo sapiens (Human)

TF-1 (cell line)

RRID:CVCL_0559

Cell line TF-1 is a Cancer cell line with a species of origin Homo sapiens (Human)

MV4-11 (cell line)

RRID:CVCL_0064

Cell line MV4-11 is a Cancer cell line with a species of origin Homo sapiens (Human)

U-937 (cell line)

RRID:CVCL_0007

Cell line U-937 is a Cancer cell line with a species of origin Homo sapiens (Human)

pLKO.1 puro (plasmid)

RRID:Addgene_8453

lentiCas9-Blast (plasmid)

RRID:Addgene_52962

lentiGuide-Puro (plasmid)

RRID:Addgene_52963

psPAX2 (plasmid)

RRID:Addgene_12260

pRSV-Rev (plasmid)

RRID:Addgene_12253

pMDLg/pRRE (plasmid)

RRID:Addgene_12251

pMD2.G (plasmid)

RRID:Addgene_12259

pLenti CMV Puro LUC (w168-1) (plasmid)

RRID:Addgene_17477

B6.SJL-Ptprc^aPepc/BoyCrCrl (organism)

RRID:IMSR_CRL:564

Mus musculus with name B6.SJL-Ptprc^aPepc/BoyCrCrl from IMSR.

NOD.Cg-Prkdc^scid Il2rg/SzJ (organism)

RRID:IMSR_JAX:005557

Mus musculus with name NOD.Cg-Prkdc^scid Il2rg/SzJ from IMSR.

NOD.Cg-Rag1^tm1Mom Il2rg Tg(CMV-IL3.CSF2.KITLG)1Eav/J (organism)

RRID:IMSR_JAX:024099

Mus musculus with name NOD.Cg-Rag1^tm1Mom Il2rg Tg(CMV-IL3.CSF2.KITLG)1Eav/J from IMSR.

MDA-MB-231 (cell line)

RRID:CVCL_0062

Cell line MDA-MB-231 is a Cancer cell line with a species of origin Homo sapiens (Human)

NB4 (cell line)

RRID:CVCL_0005

Cell line NB4 is a Cancer cell line with a species of origin Homo sapiens (Human)

Mono-Mac-6 (cell line)

RRID:CVCL_1426

Cell line Mono-Mac-6 is a Cancer cell line with a species of origin Homo sapiens (Human)

K-562 (cell line)

RRID:CVCL_0004

Cell line K-562 is a Cancer cell line with a species of origin Homo sapiens (Human)

Kasumi-1 (cell line)

RRID:CVCL_0589

Cell line Kasumi-1 is a Cancer cell line with a species of origin Homo sapiens (Human)

THP-1 (cell line)

RRID:CVCL_0006

Cell line THP-1 is a Cancer cell line with a species of origin Homo sapiens (Human)

HEK293T (cell line)

RRID:CVCL_0063

Cell line HEK293T is a Transformed cell line with a species of origin Homo sapiens (Human)