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Publication

G protein-regulated endocytic trafficking of adenylyl cyclase type 9.

eLife | 2020

GPCRs are increasingly recognized to initiate signaling via heterotrimeric G proteins as they move through the endocytic network, but little is known about how relevant G protein effectors are localized. Here we report selective trafficking of adenylyl cyclase type 9 (AC9) from the plasma membrane to endosomes while adenylyl cyclase type 1 (AC1) remains in the plasma membrane, and stimulation of AC9 trafficking by ligand-induced activation of Gs-coupled GPCRs. AC9 transits a similar, dynamin-dependent early endocytic pathway as ligand-activated GPCRs. However, unlike GPCR traffic control which requires β-arrestin but not Gs, AC9 traffic control requires Gs but not β-arrestin. We also show that AC9, but not AC1, mediates cAMP production stimulated by endogenous receptor activation in endosomes. These results reveal dynamic and isoform-specific trafficking of adenylyl cyclase in the endocytic network, and a discrete role of a heterotrimeric G protein in regulating the subcellular distribution of a relevant effector.

Pubmed ID: 32515353 RIS Download

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Associated grants

Agency: NIDA NIH HHS, United States
Id: R37 DA010711
Agency: NHLBI NIH HHS, United States
Id: K08 HL124049
Agency: NIH HHS, United States
Id: HL122508
Agency: NIDA NIH HHS, United States
Id: R29 DA010711
Agency: NIGMS NIH HHS, United States
Id: R01 GM060419
Agency: NIH HHS, United States
Id: GM133521
Agency: NCI NIH HHS, United States
Id: U54 CA209891
Agency: NIDA NIH HHS, United States
Id: R01 DA010711
Agency: NIH HHS, United States
Id: DA010154
Agency: NIDA NIH HHS, United States
Id: R01 DA012864

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