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Hemocytes in shrimp play important roles in innate immune responses against pathogens. Although three types of hemocytes including hyalinocytes, semi-granulocytes and granulocytes were identified based on their morphological characters in penaeid shrimp, knowledge about the molecular basis of their functions in the immunity is still very limited. In the present study, three subpopulations of hemocytes were firstly separated by Percoll gradient centrifugation, and their transcriptomes were analyzed. The data showed that significantly differential gene expression patterns existed in different types of hemocytes. The genes encoding phagocytic receptors, lectins and actin cytoskeleton involved in phagocytosis were highly expressed in hyalinocytes, while genes involved in the humoral immunity signaling pathways were highly expressed in semi-granulocytes, and genes encoding prophenoloxidase (proPO)-activating enzyme and serine proteases involved in proPO system activation were highly expressed in granulocytes. Further flow cytometry analysis indicated that hyalinocytes were the main hemocytes subpopulation responsible for ingesting foreign fluorescent beads, and this ingestion process mainly depends on the endocytic way of macropinocytosis. These data provide valuable information for understanding the molecular basis of distinct shrimp hemocytes subpopulations of shrimp in cellular and humoral immunity.
Pubmed ID: 32224106 RIS Download
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Web application to search protein databases using a translated nucleotide query. Translated BLAST services are useful when trying to find homologous proteins to a nucleotide coding region. Blastx compares translational products of the nucleotide query sequence to a protein database. Because blastx translates the query sequence in all six reading frames and provides combined significance statistics for hits to different frames, it is particularly useful when the reading frame of the query sequence is unknown or it contains errors that may lead to frame shifts or other coding errors. Thus blastx is often the first analysis performed with a newly determined nucleotide sequence and is used extensively in analyzing EST sequences. This search is more sensitive than nucleotide blast since the comparison is performed at the protein level.
View all literature mentionsA database for phylogenetic classification for proteins encoded in complete genomes. Clusters of Orthologous Groups of proteins (COGs) were delineated by comparing protein sequences encoded in complete genomes, representing major phylogenetic lineages. Each COG consists of individual proteins or groups of paralogs from at least 3 lineages and thus corresponds to an ancient conserved domain. Please be aware that COGs hasn't been updated in many years and will not be.
View all literature mentionsSoftware for the efficient and robust de novo reconstruction of transcriptomes from RNA-seq data.
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