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In Vitro Maturation, Fertilization, and Culture of Pig Oocytes and Embryos.

Methods in molecular biology (Clifton, N.J.) | 2019

Assisted reproductive technologies in the pig are critical for the production of genetically modified pigs as models of human disease and to improve production agriculture. Methods of oocyte maturation, fertilization, and culture all play an extremely important role in how the embryo, fetus, and offspring will develop. In this chapter, we discuss the historical methods and recent advances that have been essential in promoting efficient and competent embryo development. Here we describe the procedures that can be used to mature, fertilize, and culture pig embryos to the blastocyst stage.

Pubmed ID: 31230274 RIS Download

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Associated grants

  • Agency: NICHD NIH HHS, United States
    Id: R01 HD080636
  • Agency: NIH HHS, United States
    Id: U42 OD011140

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National Swine Resource and Research Center (tool)

RRID:SCR_006855

Provides access to critically needed swine models of human health and disease as well as a central resource for reagents, creation of new genetically modified swine, and information and training related to use of swine models in biomedical research.

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