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Evaluation of semi-automatic 3D reconstruction for studying geometry of dendritic spines.

Journal of chemical neuroanatomy | 2018

Spine geometry is considered to reflect synapse function. An accurate and fast method for 3D reconstruction of spines is considered a valuable tool for the purpose of studying spine geometry. Currently, most studies employ manual or automatic reconstruction methods, which still suffer from either poor accuracy or extreme time-consumption. The semi-automatic reconstruction method has previously been described as a time-economic and accurate tool for spine number counting. The purpose of this study is to further validate the semi-automatic method with regards to spine geometry investigation, by comparing it with the manual method as well as with the automatic method.

Pubmed ID: 30385398 RIS Download

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This is a list of tools and resources that we have found mentioned in this publication.


Imaris (tool)

RRID:SCR_007370

Imaris provides range of capabilities for working with three dimensional images. Uses flexible editing and processing functions, such as interactive surface rendering and object slicing capabilities. And output to standard TIFF, Quicktime and AVI formats. Imaris accepts virtually all image formats that are used in confocal microscopy and many of those used in wide-field image acquisition.

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NewCAST (tool)

RRID:SCR_014570

THIS RESOURCE IS NO LONGER IN SERVICE, documented on May 18, 2018; A software which provides unbiased stereological estimates of numbers, lengths, areas, and volumes present in images that were captured by microscopes or virtual slides. This software is intended to estimate structural information in a 3D format from 2D tissue sections.
There are other software tools at this company, but they have either changed names or are simply different software tools.

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