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Maturation of HIV-1 particles encompasses a complex morphological transformation of Gag via an orchestrated series of proteolytic cleavage events. A longstanding question concerns the structure of the C-terminal region of CA and the peptide SP1 (CA-SP1), which represents an intermediate during maturation of the HIV-1 virus. By integrating NMR, cryo-EM, and molecular dynamics simulations, we show that in CA-SP1 tubes assembled in vitro, which represent the features of an intermediate assembly state during maturation, the SP1 peptide exists in a dynamic helix-coil equilibrium, and that the addition of the maturation inhibitors Bevirimat and DFH-055 causes stabilization of a helical form of SP1. Moreover, the maturation-arresting SP1 mutation T8I also induces helical structure in SP1 and further global dynamical and conformational changes in CA. Overall, our results show that dynamics of CA and SP1 are critical for orderly HIV-1 maturation and that small molecules can inhibit maturation by perturbing molecular motions.
Pubmed ID: 29176596 RIS Download
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We''re called the National High Magnetic Field Laboratory because our magnets offer the highest magnetic fields for use by our international community of scientific visitors. But we could just as easily be called the National Unique Magnetic Field Laboratory, because many of our magnets and experimental techniques are highly specialized, yet broadly applicable to research in physics, materials science, chemistry, biochemistry, biology and even biomedicine. Our magnets are designed and built right here at the lab, and many are unrivalled anywhere in the world. They attract more than 1,000 visiting scientists from across the globe. Our scientists and technicians establish the experimental instrumentation and techniques that enable the scientific productivity of our user programs, which ultimately is how we, and the nation, measure our success. The only facility of its kind in the United States, the Magnet Lab is the largest and highest-powered magnet laboratory in the world, headquartered in a sprawling 370,000-square-foot complex near Florida State University in Tallahassee. The lab also includes sites at the Los Alamos National Laboratory in New Mexico and the University of Florida in Gainesville. Together these three institutions operate the lab, collaborating in a unique, interdisciplinary way to advance basic science, engineering and technology in the 21st century. the lab is a national resource open to both curious visitors and world-renowned scientists. Centralizing the country''s greatest magnet-related tools, resources and expertise is not only efficient and cost-effective, but also encourages fruitful, collaborative research at the highest level. Every year, more than 900 visiting scientists and engineers from across the world conduct experiments using our state-of-the-art equipment. Our magnets are far larger, far more powerful and far more complex than the everyday magnets most people are familiar with. Many were designed, developed and built by our magnet engineering and design team, widely recognized as the finest in the world. The Magnet Lab makes this remarkable science not just possible, but also accessible. Our doors are open to visitors, and our educational arm, the Center for Integrating Research and Learning, offers a variety of programs and resources to teachers and students at all academic levels. These efforts demonstrate our commitment to developing the next generation of science, engineering and education leaders.
View all literature mentionsSoftware package for NMR spectra acquisition, processing, and data analysis. Features include: deconvolution/spectrum simulation/iteration; comprehensive functionalities for processing, displaying and analyzing one and multi-dimensional spectra; and user customization. A full list of features is available on the website.
View all literature mentionsParallel molecular dynamics code designed for high-performance simulation of large biomolecular systems. NAMD uses the popular molecular graphics program VMD for simulation setup and trajectory analysis, but is also file-compatible with AMBER, CHARMM, and X-PLOR.
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