Hepatocellular carcinoma (HCC) is most common malignant cancer worldwide; however, the mortality rate of HCC remains high due to the invasion and metastasis of HCC. Thus, exploring novel treatments to prevent the invasion of HCC is needed for improving clinical outcome of this fatal disease. In this study, we identified lectin from Bandeiraea simplicifolia seeds (BS-I) binds to metastasis-associated HCC cell surface glycans by a lectin microarray and inhibits HCC cell migration and invasion through downregulating the matrix metalloproteinase 2 (MMP2), matrix metalloproteinase 9 (MMP9) and urokinase-type plasminogen activator (uPA) production. These effects of BS-I were mediated by inhibiting the activation of AKT/GSK-3β/β-catenin pathway and depended on specificity of lectin BS-I binding to GalNAc. GSK3β inhibitors rescued BS-I-mediated inhibition of migration and invasion of HCC cell. Further, we identified that lectin BS-I interacts with sGrp78, affects membrane localization of sGrp78 and attenuates the binding of sGrp78 and p85 to inhibit the activation of AKT/GSK-3β/β-catenin pathway. Overexpression of Grp78 or P85 rescues BS-I-mediated inhibition of migration and invasion of HCC cell. These findings demonstrated for the first time that BS-I can act as a novel potential drug to prevent the invasion of HCC.
Pubmed ID: 28922551 RIS Download
Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.
Privately held company that develops and produces antibodies, ELISA kits, ChIP kits, proteomic kits, and other related reagents used to study cell signaling pathways that impact human health.
View all literature mentionsCell line HCCLM3 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line L-02 is a Cancer cell line with a species of origin Homo sapiens
View all literature mentionsCell line Hep 3B2.1-7 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line MHCC97-L is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line MHCC97-H is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentions