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Inhibition of mitochondrial complex-1 restores the downregulation of aquaporins in obstructive nephropathy.

American journal of physiology. Renal physiology | 2016

Obstructive kidney disease is a common complication in the clinic. Downregulation of aquaporins (AQPs) in obstructed kidneys has been thought as a key factor leading to the polyuria and impairment of urine-concentrating capability after the release of kidney obstruction. The present study was to investigate the role of mitochondrial complex-1 in modulating AQPs in obstructive nephropathy. Following 7-day unilateral ureteral obstruction (UUO), AQP1, AQP2, AQP3, and vasopressin 2 (V2) receptor were remarkably reduced as determined by qRT-PCR and/or Western blotting. Notably, inhibition of mitochondrial complex-1 by rotenone markedly reversed the downregulation of AQP1, AQP2, AQP3, and V2 In contrast, AQP4 was not affected by kidney obstruction or rotenone treatment. In a separate study, rotenone also attenuated AQPs' downregulation after 48-h UUO. To study the potential mechanisms in mediating the rotenone effects on AQPs, we examined the regulation of the COX-2/microsomal prostaglandin E synthase (mPGES)-1/PGE2/EP pathway and found that COX-2, mPGES-1, and renal PGE2 content were all significantly elevated in obstructive kidneys, which was not affected by rotenone treatment. For EP receptors, EP2 and EP4 but not EP1 and EP3 were upregulated in obstructive kidneys. Importantly, rotenone strikingly suppressed EP1 and EP4 but not EP2 and EP3 receptors. However, treatment of EP1 antagonist SC-51322 could not affect AQPs' reduction in obstructed kidneys. Collectively, these findings suggested an important role of mitochondrial dysfunction in modulating AQPs and V2 receptor in obstructive nephropathy possibly via prostaglandin-independent mechanisms.

Pubmed ID: 27413198 RIS Download

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This is a list of tools and resources that we have found mentioned in this publication.


EP1 Receptor Polyclonal Antibody (antibody)

RRID:AB_10079426

This polyclonal targets EP1 Receptor

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Rabbit Anti-Rat Aquaporin 4 Polyclonal (antibody)

RRID:AB_10963604

This unknown targets Produced against the C-terminal peptide (Sequence N-CTKGSYMEVEDNRSQVETED) of rat aquaporin 4

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Rabbit Anti-Rat Aquaporin 3 Polyclonal (antibody)

RRID:AB_10963586

This unknown targets Produced against the C-terminal peptide (Sequence N-CHLEQPPPSTEAENVKLAHMKHKEQI) of rat aquaporin 3

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Rabbit Anti-Rat Aquaporin 2 Polyclonal (antibody)

RRID:AB_10965071

This unknown targets Produced against the C-terminal peptide (Sequence N-CLKGLEPDTDWEEREVRRRQ) of rat aquaporin 2

View all literature mentions

Rabbit Anti-Rat Aquaporin 1 Polyclonal (antibody)

RRID:AB_10984185

This unknown targets Produced against the N-terminal peptide (sequence N-MASEFKKKLF) of rat Aquaporin 1

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