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The western painted turtle genome, a model for the evolution of extreme physiological adaptations in a slowly evolving lineage.

H Bradley Shaffer | Patrick Minx | Daniel E Warren | Andrew M Shedlock | Robert C Thomson | Nicole Valenzuela | John Abramyan | Chris T Amemiya | Daleen Badenhorst | Kyle K Biggar | Glen M Borchert | Christopher W Botka | Rachel M Bowden | Edward L Braun | Anne M Bronikowski | Benoit G Bruneau | Leslie T Buck | Blanche Capel | Todd A Castoe | Mike Czerwinski | Kim D Delehaunty | Scott V Edwards | Catrina C Fronick | Matthew K Fujita | Lucinda Fulton | Tina A Graves | Richard E Green | Wilfried Haerty | Ramkumar Hariharan | Omar Hernandez | LaDeana W Hillier | Alisha K Holloway | Daniel Janes | Fredric J Janzen | Cyriac Kandoth | Lesheng Kong | A P Jason de Koning | Yang Li | Robert Literman | Suzanne E McGaugh | Lindsey Mork | Michelle O'Laughlin | Ryan T Paitz | David D Pollock | Chris P Ponting | Srihari Radhakrishnan | Brian J Raney | Joy M Richman | John St John | Tonia Schwartz | Arun Sethuraman | Phillip Q Spinks | Kenneth B Storey | Nay Thane | Tomas Vinar | Laura M Zimmerman | Wesley C Warren | Elaine R Mardis | Richard K Wilson
Genome biology | 2013

We describe the genome of the western painted turtle, Chrysemys picta bellii, one of the most widespread, abundant, and well-studied turtles. We place the genome into a comparative evolutionary context, and focus on genomic features associated with tooth loss, immune function, longevity, sex differentiation and determination, and the species' physiological capacities to withstand extreme anoxia and tissue freezing.

Pubmed ID: 23537068 RIS Download

Research resources used in this publication

None found

Antibodies used in this publication

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Associated grants

  • Agency: Biotechnology and Biological Sciences Research Council, United Kingdom
    Id: BB/F007590/1
  • Agency: Medical Research Council, United Kingdom
    Id: MC_U137761446
  • Agency: NIGMS NIH HHS, United States
    Id: R01 GM097251
  • Agency: NICHD NIH HHS, United States
    Id: R37 HD039963

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ShortRead (tool)

RRID:SCR_006813

Software package for input, quality assessment and exploration of high-throughput sequence data. Used for input, quality assurance, and basic manipulation of `short read'' DNA sequences such as those produced by Solexa, 454, and related technologies, including exible import of common short read data formats.

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VEGA (tool)

RRID:SCR_007907

Central repository for high quality frequently updated manual annotation of vertebrate finished genome sequence. Human, mouse and zebrafish are in the process of being completely annotated, whereas for other species the annotation is only of specific genomic regions of particular biological interest. The majority of the annotation is from the HAVANA group at the Welcome Trust Sanger Institute. Users can BLAST, search for specific text, export, and download data. Genomes and details of the projects for each species are available through the homepages for human mouse and zebrafish. The website is built upon code from the EnsEMBL (http://www.ensembl.org) project. Some Ensembl features are not available in Vega. From the users point of view perhaps the most significant of these is MartView. However due to their inclusion in Ensembl, Vega human and mouse data can be queried using Ensembl MartView. Vega contains annotation of the human MHC region in eight haplotypes, and the LRC region in three haplotypes. Vega also contains annotation on the Insulin Dependent Diabetes (IDD) regions on non-reference assemblies for mouse.

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Newbler (tool)

RRID:SCR_011916

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BLAT (tool)

RRID:SCR_011919

Software designed to quickly find sequences of 95% and greater similarity of length 25 bases or more.

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MrBayes (tool)

RRID:SCR_012067

THIS RESOURCE IS NO LONGER IN SERVICE.Documented on February 28,2023. Software program for Bayesian inference and model choice across a wide range of phylogenetic and evolutionary models.

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RepeatMasker (tool)

RRID:SCR_012954

Software tool that screens DNA sequences for interspersed repeats and low complexity DNA sequences. The output of the program is a detailed annotation of the repeats that are present in the query sequence as well as a modified version of the query sequence in which all the annotated repeats have been masked (default: replaced by Ns). Currently over 56% of human genomic sequence is identified and masked by the program. Sequence comparisons in RepeatMasker are performed by one of several popular search engines including nhmmer, cross_match, ABBlast/WUBlast, RMBlast and Decypher. RepeatMasker makes use of curated libraries of repeats and currently supports Dfam ( profile HMM library ) and RepBase ( consensus sequence library ).

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RRID:SCR_013035

Software tool for fast and high throughput alignment of shotgun cDNA sequencing reads generated by transcriptomics technologies. Fast splice junction mapper for RNA-Seq reads. Aligns RNA-Seq reads to mammalian-sized genomes using ultra high-throughput short read aligner Bowtie, and then analyzes mapping results to identify splice junctions between exons.TopHat2 is accurate alignment of transcriptomes in presence of insertions, deletions and gene fusions.

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PhyML (tool)

RRID:SCR_014629

Web phylogeny server based on the maximum-likelihood principle.

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PAML (tool)

RRID:SCR_014932

Package of programs for phylogenetic analyses of DNA or protein sequences using maximum likelihood. PAML estimates parameters and tests hypotheses to study the evolutionary process from a phylogenetic tree.

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Gblocks (tool)

RRID:SCR_015945

Software that eliminates poorly aligned positions and divergent regions of a DNA or protein alignment so that it becomes more suitable for phylogenetic analysis.

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