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Shotgun proteomic analysis of S-thiolation sites of guinea pig lens nuclear crystallins following oxidative stress in vivo.

Molecular vision | 2013

To compare levels of S-glutathiolation and S-cysteinylation occurring at more than 60 cysteine residues of 12 different guinea pig lens water-soluble nuclear crystallins following treatment of the animals with hyperbaric oxygen (HBO).

Pubmed ID: 23401655 RIS Download

Research resources used in this publication

None found

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Antibodies used in this publication

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Associated grants

  • Agency: NEI NIH HHS, United States
    Id: R01 EY007755
  • Agency: NEI NIH HHS, United States
    Id: P30 EY010572
  • Agency: NEI NIH HHS, United States
    Id: R01EY02027
  • Agency: NEI NIH HHS, United States
    Id: R01EY007755
  • Agency: NEI NIH HHS, United States
    Id: R24EY014803
  • Agency: NEI NIH HHS, United States
    Id: R01 EY002027
  • Agency: NEI NIH HHS, United States
    Id: P30EY10572
  • Agency: NEI NIH HHS, United States
    Id: R24 EY014803

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FASTA (tool)

RRID:SCR_011819

Software package for DNA and protein sequence alignment to find regions of local or global similarity between Protein or DNA sequences, either by searching Protein or DNA databases, or by identifying local duplications within a sequence.

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BioWorks (tool)

RRID:SCR_014594

A a configurable software package for peptide and protein mass spectrometry analyses. It includes the SEQUEST search algorithm to identify separate proteins in complex mixtures, interactive navigation tools to filter and sort protein summaries, customized spectral plots, and chromatograms using the PEPMATCH and PEPMAP tools. This software also has batch processing capabilities to improve throughput by queuing up several files, and custom-build proprietary databases, index databases, and retrieve databases through a public server.

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