URL: http://antibodyregistry.org/AB_715124
Proper Citation: Novus Cat# NB110-40763, RRID:AB_715124
Target Antigen: TRPA1
Host Organism: Rabbit
Clonality: polyclonal
Comments:
Applications: Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen
Consolidation on 5/2023: AB_1291838, AB_10184769, AB_10371444.
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No rating or validation information has been found for TRPA1 Antibody - BSA Free.
Issue
ISSUE: In the conditions used, this antibody was able to detect overexpressed TRPA1 in HEK293T cells by western blotting, however multiple non-specific strong bands were seen, including close to the molecular weight of the specific bands - making interpretation difficult. In immunofluorescence there is a weak correlation between antibody staining and the GFP reporter for TRPA1 - suggesting some sensitivity, but poor specificity in the conditions used.
Source: Antibody Registry
