URL: http://www.wormbase.org/db/get?name=WBStrain00024074
Proper Citation: RRID:WB-STRAIN:WBStrain00024074
Description: Caenorhabditis elegans with name pha-1(e2123) III; him-5(e1490) V; rnyEx109. from WB.
Species: Caenorhabditis elegans
Synonyms: pha-1(e2123) III; him-5(e1490) V; rnyEx109.
Notes: rnyEx109 [nhx-2p::D3cpv + pha-1(+)]. Maintain at 20-25C to select for array. KWN190 strain expresses the FRET-based calcium indicator protein D3cpv throughout the cytoplasm of intestinal cells. Dual emission ratio imaging (ex. 435, em. 480/535-nm) can be used to measure intestinal calcium and, although the FRET pair is CFP/YFP, intestinal D3cpv fluorescence is observable under standard GFP filter sets. The D3cpv biosensor has the advantages of being relatively pH-insensitive and not interfering with endogenous calmodulin signaling. References: Am J Physiol Cell Physiol. 301:C1389-1403. Am J Physiol Cell Physiol. 297:C1071-81. FASEB J. 27:760-768. PLoS Biol. 11: e1001613.|"WBStrain provided so WBPaper00061870 paper added based on AFP_Strain data."
Affected Gene: WBGene00001864(him-5)|WBGene00004010(pha-1)
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No rating or validation information has been found for KWN190.
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Source: Integrated Animals
Source Database: WormBase (WB)
