Harvard Medical School Dana Farber Harvard Cancer Center Mouse Engineering Core Facility (RRID:SCR_022950)Copy Citation Copied
URL: https://www.dfhcc.harvard.edu/research/core-facilities/mouse-engineering/
Proper Citation: Harvard Medical School Dana Farber Harvard Cancer Center Mouse Engineering Core Facility (RRID:SCR_022950)
Description: Core provides services for generation of transgenic and knockout mice.Provides Nikon Diaphot microscope equipped with Nomarski Optics and Narishige micromanipulators for microinjections, Nikon surgical microscopes for egg isolation and transfer, sutter needle puller and de Fonbrune microforge. Facility maintains mice necessary for egg donors, egg recipients, and vasectomized males.Core offers electroporation of targeting vectors into ES cells. Provides investigators with DNA to identify ES cells carrying desired recombination events and ES cell expansion for microinjection into blastocysts.Services also include CRISPR injections, sperm and embryo cryopreservation, and reanimation with fresh or frozen sperm via IVF or thawing and implanting embryos.
Synonyms: Harvard Medical School DF/HCC Mouse Engineering Core, DF/HCC Mouse Engineering Core
Resource Type: service resource, access service resource, core facility
Keywords: USEDit, ABRF, generation of transgenic and knockout mice
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