URL: http://www.scienceexchange.com/facilities/high-throughput-clinical-proteomics-core-ucla
Proper Citation: UCLA High-Throughput Clinical Proteomics Core (RRID:SCR_012205)
Description: THIS RESOURCE IS NO LONGER IN SERVCE, documented September 6, 2016. The High-Throughput Clinical Proteomics (HTCP) core laboratory is a mass spectrometry core facility designed to profile large numbers of clinical samples for changes in protein content. We use small amounts (micrograms) of protein from samples such as urine, plasma, CSF, and cell lysates and incubate them with magnetic beads that bind different subsets of the total proteome. We then examine the different subsets of small proteins and peptides by high-resolution MALDI-TOF mass spectrometry. We measure the amounts and intact masses of the proteins in the samples and determine which have the greatest statistical relevance in defining clinical groupings before moving to physically isolate those peptides/proteins and identify them. The method works best for peptides and small proteins below 25 kDa and is an excellent complement to gel-based methods such as DIGE.
Abbreviations: UCLA HTCP, UCLA HTCP Core
Synonyms: University of California Los Angeles High-Throughput Clinical Proteomics Core
Resource Type: core facility, access service resource, service resource
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