URL: http://hawaii.eagle-i.net/i/0000012b-3599-5885-2f73-b43980000000
Proper Citation: UH Manoa Sequencing Facility (RRID:SCR_010114)
Description: Core facility that provides the following services: Construction and validation of random-sheared DNA fragment libraries, DNA sequencing (Sanger) service, 96-well plasmid preparation service, Genotyping/fragment analysis, BAC end sequencing, Plasmid library construction service, Genome sequencing project. The ASGPB genome infrastructure currently consists of several state-of-the-art capillary-based DNA sequencers and accessory equipment to deal with large sample volumes. The facility can process up to 9000 samples per day with a projected sequence output of 6.75 Million bases. Several prokaryotic and one eukaryotic genome project are currently in progress. The facility offers DNA sequencing services for a wide range of templates including plasmid DNA, PCR fragments, cosmid DNA, and Bacterial Artificial Chromosomes (BACs). Sequencing reactions are performed using Applied Biosystems BigDye terminator chemistry and are run on ABI 3730XL capillary-based DNA sequencers.
Resource Type: service resource, access service resource, core facility
Keywords: library preparation, dna sequencing, plasmid purification, genotyping assay, nucleic acid fragment analysis, sequencing assay, next generation sequencing
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