URL: http://hawaii.eagle-i.net/i/0000012a-2510-aae4-c99c-aef680000000
Proper Citation: UH Manoa COBRE Transgenic Core (RRID:SCR_010093)
Description: Core facility that provides the following services: Transgenic mouse generation, In vitro fertilization (IVF) service, Embryo culture service, Embryo freezing service. The Transgenic and Embryonic Stem Cell Gene Targeting Core is a state-of-the-art facility with the expertise in the production of genetically altered subjects. Transgenic subjects carrying new or novel genes are created by microinjection of DNA into the pronuclei of fertilized eggs. Knock-out lacking specific genes of interest are created by homologous recombination in embryonic stem cells followed by injection into blastocysts to create chimeric subjects. Highly experienced personnel produce transgenic and knock-out subjects for UH investigators at very reasonable cost and with very short lead times. (http://www.ibr.hawaii.edu/tc.html)
Resource Type: service resource, access service resource, core facility
Keywords: embryo transplantation, oocyte injection, in vitro fertilization, embryo culture, cryoembedding
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