URL: http://eagle-i.itmat.upenn.edu/i/00000138-7bcc-1f66-fbab-3b8480000000
Proper Citation: Penn Cell Center Services Facility (RRID:SCR_010002)
Description: Core facility that provides the following services: Antibody screening by ELISA, Cell Culture Training, Production and Purification of Antibodies from Hybridoma Lines, Endotoxin Testing, EBV Transformation, Special media production, Liquid N2 storage, Freezing Cells. Cell Center Services Facility is the service component of the Cell Center, provides training and services in various cell culture and associated procedures including Mycoplasma and Endotoxin testing. The cell culture service includes cell culture at various scales, large scale growth of hybridoma and other cell lines followed by antibody purification by protein G column and the generation of lymphoblastoid cell lines by EBV induced transformation of lymphocytes. In addition, the facility prepares specialized cell culture media, drosophila media, and various molecular biological reagents. Recently cell transfection and selection service has been introduced at the facility.
Resource Type: access service resource, core facility, service resource
Keywords: elisa, maintaining cell culture, antibody production, antibody purification, microbial testing, cell immortalization, ebv transformation, material storage, cryopreservation
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