URL: http://harvard.eagle-i.net/i/0000012e-9652-3f24-55da-381e80000000
Proper Citation: MGH Confocal Microscope Core (RRID:SCR_009921)
Description:
Core facility that provides the following services: Double- and triple-labeling experiments, Acquisition of high-resolution images: 2048 x 2048 pixels, Single molecule visualization, allowing dynamic observation and functional analyses of both in vivo and living cells, Total internal reflection fluorescence experiments (TIRF).
This Core consists of a Zeiss LSM 5 Pascal laser confocal microscope with a Zeiss RGB vario laser module and Nikon C1 Confocal/TIRF System with 3 PMT. A Zeiss Axiovert 200 fully motorized light microscope is available with fluorescence, bright-field, phase-contrast and Nomarski (DIC) capabilities. Image acquisition and analyses are performed using Zeiss LSM 5 Pascal Confocal Microscopy Software (Release 3.2) on 2 workstations. Zeiss Physiology software is available also. Live cell imaging is available using a Zeiss temperature controller with custom chamber and heating stage. The Nikon C1 Confocal/TIRF System fully motorized Nikon Eclipse Ti microscope is available with fluorescence, bright-field and TIRF capabilities. Imaging acquisition and analyses are performed using EZ-C1 and NIC-Elements Software.
Resource Type: service resource, access service resource, core facility
Keywords: immunofluorescence microscopy assay, imaging technique
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