URL: http://www.ncbi.nlm.nih.gov/tools/primer-blast/
Proper Citation: Primer-BLAST (RRID:SCR_003095)
Description: A tool to design target-specific primers for polymerase chain reaction (PCR). It uses Primer3 to design PCR primers and then uses BLAST and global alignment algorithm to screen primers against user-selected database in order to avoid primer pairs (all combinations including forward-reverse primer pair, forward-forward as well as reverse-reverse pairs) that can cause non-specific amplifications.
Abbreviations: Primer-BLAST
Resource Type: production service resource, analysis service resource, data analysis service, service resource
Defining Citation: PMID:22708584
Keywords: primer, blast, pcr target, polymerase chain reaction, primer design
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